Eur. J. Entomol. 110 (1): 1-12, 2013 | DOI: 10.14411/eje.2013.001

Cytogenetic variability among Bradyporinae species (Orthoptera: Tettigoniidae)

Elżbieta WARCHAŁOWSKA-ŚLIWA1, Beata GRZYWACZ1, Anna MARYAŃSKA-NADACHOWSKA1, Tatyana V. KARAMYSHEVA2, Dragan P. CHOBANOV3, Klaus-Gerhard HELLER4
1 Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Kraków, Poland; e-mails: warchalowska@isez.pan.krakow.pl; grzywacz@isez.pan.krakow.pl; maryanska@isez.pan.krakow.pl
2 Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; e-mail: kary@bionet.nsc.ru
3 Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences , Tsar Osvoboditel 1, 1000 Sofia, Bulgaria; e-mail: dchobanov@gmail.com
4 Grillenstieg 18, 39120 Magdeburg, Germany; e-mail: Heller.volleth@T-Online.de

Chromosomes of six European species (one with two subspecies) of Orthoptera belonging to the tribes Ephippigerini and Bradyporini were analyzed using C-banding, Ag-NOR, DAPI (AT-rich)/CMA3 (GC-rich) staining and fluorescence in situ hybridization (FISH) using the 18S rDNA and (TTAGG)n telomeric probes with the aim to better understand chromosomal organization and evolutionary relationships between genera and subgenera within and across both tribes. The evolution of karyotypes was studied in terms of changes in chromosome number (2n) and morphology (FN, the fundamental number - i.e. the number of chromosome arms including the X chromosome). The ancestral 2n = 31 was reduced to 2n = 29 (FN = 31) and 27 (FN = 31) by one or two Robertsonian fusions in the Ephippigerini. Whereas in the Bradyporini 2n = 27 (FN = 32) as a result of two Robertsonian translocations and a pericentric inversion in the X chromosome. The quantity of heterochromatin in GC-rich regions distinguished the karyotypes of Ephippigerini (only a single CG-rich band on one autosome pair) from those of Bradyporini (CG-rich bands on all chromosomes). FISH using the 18S rDNA probe localized 1-3 rDNA clusters to autosomes and/or to the X chromosome in all species examined. The rDNA loci coincided with active NORs as determined by Ag-NOR staining. A comparison of the location of the single NOR/rDNA in two species of the genus Steropleurus (Ephippigerini) suggests that the reduced chromosome number in S. pseudolus results from a Robertsonian fusion between two pairs of autosomes, one of them carrying the NOR/rDNA as in S. stalii (and also in E. ephippiger). Whereas the karyotypes of three species of the genus Bradyporus, though showing the same chromosome number and morphology, differed in the number and distribution of NORs/rDNA sites [one autosomal in B. (B.) dasypus versus three in B. macrogaster and B. (C.) oniscus, two of them X-linked]. Trends in karyotype diversification of the taxa based on the present data and previous research are discussed. In some individuals belonging to the species Bradyporus (B.) dasypus and B. (C.) m. macrogaster B chromosomes (Bs) were detected: acrocentric (the smallest elements in the complement) and submetacentric (similar to medium-sized autosomes), respectively.

Keywords: Orthoptera, Ephippigerini, Bradyporini, karyotype evolution, chromosome fusion, B chromosomes, FISH, rDNA

Received: June 13, 2012; Revised: August 8, 2012; Accepted: August 8, 2012; Published: January 2, 2013  Show citation

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WARCHAŁOWSKA-ŚLIWA, E., GRZYWACZ, B., MARYAŃSKA-NADACHOWSKA, A., KARAMYSHEVA, T.V., CHOBANOV, D.P., & HELLER, K. (2013). Cytogenetic variability among Bradyporinae species (Orthoptera: Tettigoniidae). EJE, 110(1), 1-12. doi: 10.14411/eje.2013.001
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    References

    1. BARAT J. 2007: Revision taxonomica de los Ephippigerinae (Orthoptera: Tettigonioidea: Bradyporidae) de da Peninsula Iberica e Islas Baleares. I. Generos: Callicrania Bolivar, 1898; Neocallicrania Pfau, 1996; Platystolus Bolivar, 1878 y Synephippius NavLs, 1905. - Bol. Soc. Entomol. Aragon. 40: 55-118
    2. BRESSA M.J., FRANCO M.J., TOSCANI M.A. & PAPESCHI A.G. 2008: Heterochromatin polymorphism in Holhymenia rubiginosa (Heteroptera: Coreidae). - Eur. J. Entomol. 105: 65-72 Go to original source...
    3. BUGROV A.G., NOVIKOVA O.S., NETESOVA E.S., GOROCHOV A.V. & BLINOV A.G. 2009: Using DNA sequences of two mitochondrial genes (COI and COII) for taxonomy and phylogeny of Bradyporinae katydids (Orthoptera, Ensifera, Tettigoniidae). - Euroasian Entomol. J. 8: 1-8 [in Russian]
    4. CABRAL-DE-MELLO D.C., OLIVEIRA S.B., MOURA R.C. & MARTINS C. 2011: Chromosomal organization of the 18S and 5S rRNAs and histone H3 genes in Scarabaeinae coleopterans: insights into the evolutionary dynamics of multigene families and heterochromatin. - BMC Genetics 12: 88 Go to original source...
    5. CAMACHO J.P.M., SHARBEL T.F. & BEUKEBOOM L. 2000: B-chromosome evolution. - Phil. Trans. R. Soc. Lond. (B) 355: 163-178 Go to original source...
    6. CEJCHAN A. 1967: A taxonomic study in Deracanthinae (Orthoptera, Bradyporidae). - Acta Entomol. Mus. Nat. Prag. 37: 607-633
    7. EADES D.C., OTTE D., CIGLIANO M.M. & BRAUN H. 2012: Orthoptera Species File Online. Version 2.0/4.1. http://Orthoptera.SpeciesFile.org. 2012/04/08
    8. FERNANDEZ-PIQUERAS J., ROJO GARCIA E. & SENTIS CASTANO C. 1981: A tandem fusion origin of a neo XY sex determining mechanism in the longhorned Callicrania seoanei (Bol.). - Heredity 47: 397-401 Go to original source...
    9. FERNANDEZ-PIQUERAS J., RODRIGUEZ CAMPOS A., SENTIS CASTANO C. & WANDOSELL JURADO F. 1982: Pycnogaster cucullata (Charp): a polytypic species of Tettigonioidea with X0 and neo XY sex determination. - Heredity 48: 147-150 Go to original source...
    10. FERNANDEZ-PIQUERAS J., RODRIGUEZ CAMPOS A. & SENTIS CASTANO C. 1983a: Hypotheses about speciation by chromosomal rearrangements in the Steropleurus martorelli complex (Tettigonioidea, Orthoptera). - Genetica 60: 167-172 Go to original source...
    11. FERNANDEZ-PIQUERAS J., RODRIGUEZ CAMPOS A., SENTIS CASTANO C. & ROJO-GARCIA E. 1983b: Sex chromosome evolution in the polytypic species Pycnogaster cucullata. - Heredity 50: 217-223 Go to original source...
    12. FERNANDEZ-PIQUERAS J., RODRIGUEZ CAMPOS A., SENTIS CASTANO C. & ROJO-GARCIA E. 1983c: Chromosomal location of the active NOR in the Steropleurus martorelli complex. - Genetica 61: 9-12 Go to original source...
    13. FERNANDEZ-PIQUERAS J., RODRIGUEZ A., SENTIS CASTANO C. & ROJO-GARCIA E. 1984: Cheterochromatin variation in the monospecific genus Baetica (Orthoptera: Tettigoniidae). - Caryologia 37: 69-76 Go to original source...
    14. LEMONNIER-DARCEMONT M., DUTRILLAUX A.-M., DUTRILLAUX B. & DARCEMONT C. 2009: Summary of knowledge on Bradyporis dasypus (Illiger, 1880) (Orthoptera: Tettigoniidae). - Articulata 24: 15-29
    15. LEVAN A., FREDGA K. & SANDBERG A.A. 1964: Nomenclature for centromeric position of chromosomes. - Hereditas 52: 201-220 Go to original source...
    16. LOPEZ-FERNANDEZ C., PRADILLO E., ZABAL-AGUIRRE M., FERNANDEZ J.L., GARCIA DE LA VEGA C. & GISAVELEZ J. 2004: Telomeric and interstitial telomeric-like DNA sequence in Orthoptera genomes. - Genome 47: 757-763 Go to original source...
    17. LORETO V. & SOUZA M.J. 2010: Karyotype, constitutive heterochromatin and nucleolar organizer regions (NORs) in Belosacris coccineipes (Acrididae - Leptysminae). - Gen. Mol. Biol. 23: 575-579 Go to original source...
    18. NADIG A. 1994: Revision of the genus Uromenus Bolivar, 1878 (Orthoptera: Ephippigeridae). - Rev. Suisse Zool. 101: 919-1016 Go to original source...
    19. NAVAS-CASTILLO J., CABRERO J. & CAMACHO J.P.M. 1986: Heterochromatin variation in Beatica ustulata (Orthoptera: Tettigoniidae) analysed by C and G-banding. - Heredity 56: 161-165 Go to original source...
    20. PEINADO M.V. 1990: Tettigonioideos espanoles (Ephippigerinae). PhD Thesis, Universidad Complutense de Madrid, 411 pp
    21. PFAU H.K. 1988: Untersuchungen zur Stridulation und Phylogenie der Gattung Pycnogaster Graells, 1851 (Orthoptera, Tettigoniidae, Pycnogasterinae). - Mitt. Schweizer. Entomol. Ges. 61: 167-183
    22. PFAU H.K. 1996: Bioacoustics and evolution of Platystolus Bolivar (Ensifera, Tettigoniidae). - Tijdschr. Entomol. 139: 33-72
    23. PFAU H.K. & PFAU B. 1995: Bioacoustics and evolution of the Pycnogastrinae (Orthoptera, Tettigoniidae): Pycnogaster valentini Pinedo and Llorente, 1986 and Pycnogaster cucullata (Charpentier, 1825). - Mitt. Schweizer. Entomol. Ges. 68: 465-478
    24. SCHNEIDER M.C., ROSA S.P., ALMEIDA M.C., COSTA C. & CELLA D.M. 2007: Chromosomal similarities and differences among four Neotropical Elateridae (Conoderini and Pyrophorini) and other related species, with comments on the NOR pattern in Coleoptera. - J. Zool. Syst. Evol. Res. 45: 308-316 Go to original source...
    25. SCHWEIZER D. 1976: Reverse fluorescent chromosome banding with chromomycin and DAPI. - Chromosoma 58: 307-324 Go to original source...
    26. SENTIS C. & FERNANDEZ-PIQUERAS J. 1985: The B chromosomes of Pycnogaster cucullata (Charp.) and their effects on the A complement. - Heredity 55: 375-380 Go to original source...
    27. SENTIS C., SANTOS J. & FERNANDEZ-PIQUERAS J. 1986: C-heterochromatin polymorphism in Baetica ustulata: intraindividual variation and fluorescence banding pattern. - Chromosoma 94: 65-70 Go to original source...
    28. SUMNER S.G. 1972: A simple technique for demonstrating centromere heterochromatin. - Exp. Cell. Res. 75: 304-306 Go to original source...
    29. SUMNER A.T. 1990: Chromosome Banding. Unwin Hyman, London, 434 pp
    30. TERUEL M., CABRERO J. PERFECTTI F. & CAMACHO J.P.M. 2010: B chromosome ancestry revealed by histone genes in the migratory locust. - Chromosoma 119: 217-225 Go to original source...
    31. TURKOGLU S. & KACA S. 2002: Karyotype, C- and G-band patterns and DNA content of Callimenus (= Bradyprus) macrogaster macrogaster. - J. Insect. Sci. 2:24, Available online: insectscience.org/2.24 Go to original source...
    32. UNAL M. 2011: Taxonomic review of the subfamily Bradyporinae (Orthoptera: Tettigoniidae; Bradyporini; Ephippigerini) of Turkey, with description of new species and the relationship of the taxa. - Zootaxa 2899: 1-42 Go to original source...
    33. WARCHALOWSKA-SLIWA E. 1998: Karyotype characteristics of katydid orthopterans (Ensifera, Tettigoniidae) and remarks on their evolution at different taxonomic levels. - Folia Biol. (Krakow) 46: 143-176
    34. WARCHALOWSKA-SLIWA E. & BUGROV A.G. 1996: Karyotypes and C-banding patterns of Bradyporinae (Orthoptera, Tettigoniidae). - Folia Biol. (Krakow) 44: 95-98
    35. WARCHALOWSKA-SLIWA E. & BUGROV A.G. 1997: C-heterochromatin variation in Deracantha onos (Pall.) (Deracanthini, Bradyporinae, Tettigoniidae, Orthoptera). - Cytologia 62: 7-12. Go to original source...
    36. WARCHALOWSKA-SLIWA E. & MARYANSKA-NADACHOWSKA A. 1992: Karyotypes, C-bands, NORs location in spermatogenesis of Isophya brevipennis Brunner (Orthoptera: Phaneropteridae). - Caryologia 45: 83-89 Go to original source...
    37. WARCHALOWSKA-SLIWA E., CHOBANOV D., GRZYWACZ B. & MARYANSKA-NADACHOWSKA A. 2008: Taxonomy of the genus Isophya (Orthoptera, Phaneropteridae, Barbitistinae): Comparison of karyological and morphological data. - Folia Biol. (Krakow) 56: 227-241 Go to original source...
    38. WARCHALOWSKA-SLIWA E., GRZYWACZ B., MARYANSKANADACHOWSKA A., KARAMYSHEVA T.V., RUBTSOV N.B. & CHOBANOV D.P. 2009: Chromosomal differentiation among bisexual European species of Saga (Orthoptera, Tettigoniidae, Saginae) detected by both classical and molecular methods. - Eur. J. Entomol. 106: 1-9 Go to original source...
    39. WARCHALOWSKA-SLIWA E., MARYANSKA-NADACHOWSKA A., GRZYWACZ B., KARAMYSHEVA T., LEHMANN A.W., LEHMANN G.U.C. & HELLER K.-G. 2011: Changes in the numbers of chromosomes and sex determination system in bushcrickets of the genus Odontura (Orthoptera, Tettigoniidae, Phaneropterinae). - Eur. J. Entomol. 108: 183-195 Go to original source...
    40. ZHOU Z., HUANG Y., SHI F. & YE H. 2009: The complete mitochondrial genome of Deracantha onos (Orthoptera: Bradyporinae). - Mol. Biol. Rep. 36: 7-12 Go to original source...

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