Eur. J. Entomol. 121: 40-53, 2024 | DOI: 10.14411/eje.2024.007

DNA metabarcoding of gut microbiota reveals considerable taxonomic differences among wild individuals of the dung beetle Trypocopris pyrenaeus (Coleoptera: Geotrupidae)Original article

Gianluca NATTA ORCID...1, Samuele VOYRON ORCID...2, Erica LUMINI ORCID...3, Alex LAINI ORCID...1, Alfredo SANTOVITO ORCID...1, Angela ROGGERO ORCID...1,*, Claudia PALESTRINI ORCID...1, 4, Antonio ROLANDO ORCID...1
1 Department of Life Science and Systems Biology (DBIOS), Turin University, Via Accademia Albertina 13, 10123 Turin, Italy; e-mails: gianluca.natta@unito.it, alex.laini@unito.it, alfredo.santovito@unito.it, angela.roggero@unito.it, claudia.palestrini@unito.it, antonio.rolando@unito.it
2 Department of Life Science and Systems Biology (DBIOS), Turin University, Viale Pier Andrea Mattioli 25, 10125 Turin, Italy; e-mail: samuele.voyron@unito.it
3 Institute for Sustainable Plant Protection (IPSP) - National Research Council (CNR), Viale Pier Andrea Mattioli 25, 10125 Turin, Italy; e-mail: erica.lumini@ipsp.cnr.it
4 NBFC, National Biodiversity Future Center, Palermo 90133, Italy

The gut microbiota of dung beetles comprises bacteria, archaea, and fungi. Most studies have only considered the bacterial element and focused on differences between species reared in the lab. This study considered microorganisms from the gut of Trypocopris pyrenaeus and concentrated on differences among wild individuals of an alpine Italian population. We revealed remarkable interindividual variation in the taxonomic composition of microbiota. Core taxa were few, while individuals harboured many exclusive taxa. Therefore, considering only a few individuals to describe the microbiota of a species is not sufficient. The study also revealed that the nMDS ordination of individuals based on prokaryotes was different from that based on fungi, meaning that both groups should be considered in microbiota analyses and that one microbic group cannot be considered a surrogate of the other. We identified many functions potentially provided by the microbiota and found the taxonomic richness of prokaryotes to be positively correlated with their functional richness. The analysis of the functions potentially provided by these microorganisms confirmed that the gut microbiota, in addition to being essential for the health of their host, may also contribute to the effective functioning of the ecosystems of which dung beetles are part.

Keywords: Digestive tract, coprophagy, ecological functions, bacteria, archaea, fungi, alpha diversity, functional diversity, core taxa

Received: October 27, 2023; Revised: January 21, 2024; Accepted: January 21, 2024; Published online: February 15, 2024  Show citation

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NATTA, G., VOYRON, S., LUMINI, E., LAINI, A., SANTOVITO, A., ROGGERO, A., PALESTRINI, C., & ROLANDO, A. (2024). DNA metabarcoding of gut microbiota reveals considerable taxonomic differences among wild individuals of the dung beetle Trypocopris pyrenaeus (Coleoptera: Geotrupidae). EJE, 121, Article 40-53. https://doi.org/10.14411/eje.2024.007
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