Eur. J. Entomol. 120: 297-303, 2023 | DOI: 10.14411/eje.2023.030

Establishment of a primary cell culture of Thrips palmi (Thysanoptera: Thripidae)Short Communication

Amalendu GHOSH ORCID...1, Heena DHALL1, Sumit JANGRA1, 2
1 Insect Vector Laboratory, Advanced Centre for Plant Virology, ICAR - Indian Agricultural Research Institute (IARI), New Delhi 110 012, India; e-mails: amal4ento@gmail.com, heenadhall@yahoo.com, sumit.jangra712@gmail.com
2 Tropical Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Homestead - 33031, FL, USA

Thrips palmi (Thysanoptera: Thripidae) is an important pest of vegetables, ornamental plants and fruit crops. In addition to the direct damage caused by feeding, it transmits several tospoviruses. The absence of an in vitro assay system is a major bottleneck in investigating thrips-tospovirus interactions. The present study reports the establishment of a primary cell culture of T. palmi, which was initiated using embryonic tissue as an explant in modified Kimura's medium. Fibroblast-like cells began to be produced 3 h after tissue implantation and were the dominant cell type. They grew in size and number and covered most of the surface. This primary cell culture survived for 37 days providing sufficient time for analytical molecular studies on the replication of tospovirus and interactions with the vector components.

Keywords: Melon thrips, in vitro, virus-vector relationship, fibroblast-like cells, orthotospovirus

Received: March 13, 2023; Revised: August 14, 2023; Accepted: August 14, 2023; Published online: August 30, 2023  Show citation

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GHOSH, A., DHALL, H., & JANGRA, S. (2023). Establishment of a primary cell culture of Thrips palmi (Thysanoptera: Thripidae). EJE, 120, Article 297-303. https://doi.org/10.14411/eje.2023.030
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