Eur. J. Entomol. 119: 354-361, 2022 | DOI: 10.14411/eje.2022.036

Seasonal and geographical adaptations in the parthenogenetic stick insect, Ramulus mikado (Phasmatodea: Phasmatidae)Ivo Hodek special issueOriginal article

Keiji NAKAMURA, Yuuki FUKUSHIMA
Department of Biosphere-Geosphere System Science, Faculty of Informatics, Okayama University of Science, Okayama 700-0005, Japan; e-mails: nakamura@ous.ac.jp, yukifukushima222@gmail.com

Seasonal and geographical adaptations in terms of obligatory embryonic diapause in the parthenogenetic stick insect, Ramulus mikado, were studied. First and second instar nymphs were collected at locations at three latitudes in Japan and reared in the laboratory under a photoperiod of 16L : 8D or 12L : 12D at 25°C. Their eggs were kept at 30°C for 30 or 60 days after oviposition, but no eggs hatched. Hatching was observed more than 100 days after transfer from 30°C to 15°C. The long period between transfer and hatching indicate that eggs in an early embryonic stage of development enter diapause at high-temperatures. The time from oviposition to hatching of eggs laid by adults that originated from the three locations kept under constant conditions between 15 and 25°C were compared. In all these experiments, eggs laid by individuals originating from high latitudes took longer to hatch. The eggs of those originating from Okayama and Ehime did not hatch at 25°C. However, more than 80% of the eggs of those that originated from the northernmost population hatched. Hatching before winter was observed when the eggs of those that originated from the northern population were placed outdoors in Okayama, even when the maternal insects were reared under long-day conditions in the laboratory. These findings indicate that univoltine R. mikado enters diapause twice during embryonic development, which enables it survive adverse conditions in summer and winter, respectively. Furthermore, diapause intensity was lowest in insects that originated from the lowest latitude.

Keywords: Diapause development, diapause intensity, diapause termination, egg diapause, photoperiod, temperature

Received: June 4, 2022; Revised: August 4, 2022; Accepted: August 4, 2022; Published online: August 25, 2022  Show citation

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NAKAMURA, K., & FUKUSHIMA, Y. (2022). Seasonal and geographical adaptations in the parthenogenetic stick insect, Ramulus mikado (Phasmatodea: Phasmatidae). EJE, 119, Article 354-361. https://doi.org/10.14411/eje.2022.036

This paper was contributed to a virtual special issue in memory of Ivo Hodek, a long-time editor of the European Journal of Entomo­logy, who died on June 11, 2021, shortly after his ninetieth birthday.

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    References

    1. Ando Y. 1983: Diapause and geographic variation in a leaf beetle. In Brown V.K. & Hodek I. (eds): Diapause and Life Cycle Strategies in Insects. W. Junk, The Hague, pp. 127-141.
    2. Andrewartha H.G. 1952: Diapause in relation to the ecology of insects. - Biol. Rev. 27: 50-107. Go to original source...
    3. Bedford G.O. 1970: The development of the egg of Didymuria cuilescens (Phasmatodea: Phasmatidae: Podacanthinae) - embryology and determination of the stage at which first diapause occurs. - Austral. J. Zool. 18: 155-169. Go to original source...
    4. Bedford G.O. 1978: Biology and ecology of the Phasmatodea. - Annu. Rev. Entomol. 23: 125-149. Go to original source...
    5. Danilevsky A.S. 1961: [Hidaka T. & Masaki S. Japanese translation, 1966] Photoperiodism and Seasonal Development of Insects. University of Tokyo Press, Tokyo, 293 pp.
    6. Grüner C. & Sauer K.P. 1988: Aestival dormancy in the cabbage moth Mamestra brassicae L. (Lepidoptera: Noctuidae). - Oecologia 74: 515-523. Go to original source...
    7. Hodek I. 1996: Diapause development, diapause termination and the end of diapause. - Eur. J. Entomol. 93: 475-487.
    8. Hodek I. & Hodková M. 1988: Multiple role of temperature during insect diapause: a review. - Entomol. Exp. Appl. 49: 153-165. Go to original source...
    9. Lee J., Baek S., Kang C., Lee Y.S., Lee Y., & Lee J.H. 2018: Temperature-dependent development and oviposition models of Ramulus irregulariterdentatus (Phasmida: Phasmatidae). - J. Asia-Pacific Entomol. 21: 903-913. Go to original source...
    10. Lee J.G., Kim H.D., Kang C.S., Seo A.G., Lee S.H., Park Y.K. & Lee Y.B. 2013: Study of the hatching characteristics and diet of the stick insect, Baculum elongatum (Phasmida: Phasmatidae) for artificial mass rearing. - Int. J. Ind. Entomol. 26: 61-66. Go to original source...
    11. Margaritopoulos J.T. & Tzanakakis M.E. 2006: Diapause completion in the almond seed wasp, Eurytoma amygdali (Hymenoptera: Eurytomidae) following early low temperature treatment. - Eur. J. Entomol. 103: 733-742. Go to original source...
    12. Masaki S. 1956: The local variation in the diapause pattern of the cabbage moth, Barathra brassicae Linné, with special reference to the aestival diapause (Lepidoptera: Noctuidae). - Bull. Fac. Agr. Mie Univ. 13: 29-46.
    13. Masaki S. 1965: Geographic variation of diapause in the intrinsic incubation period: a physiological cline in the Emma field cricket (Orthoptera: Gryllidae: Teleogryllus). - Bull. Fac. Agr. Hirosaki Univ. 11: 59-90.
    14. Masaki S. 1980: Summer diapause. - Annu. Rev. Entomol. 25: 1-25. Go to original source...
    15. Masaki S. 1999: Seasonal adaptations of insects as revealed by latitudinal diapause clines. - Entomol. Sci. 2: 539-549.
    16. Masaki S. 2002: Ecophysiological consequences of variability in diapause intensity. - Eur. J. Entomol. 99: 143-154. Go to original source...
    17. Nozaki M., Suetsugu K., Sato K., Sato R., Takagi T., Funaki S., Ito K., Kurita K., Isagi Y. & Kaneko S. 2021: Development of microsatellite markers for the geographically parthenogenetic stick insect Phraortes elongatus (Insecta: Phasmatodea). - Genes Genet. Syst. 96: 199-203. Go to original source...
    18. Okada M. 1999: All about Stick Insects. Tombow Publishing, Osaka, 55 pp.
    19. Readshaw J.L. & Bedford G.O. 1971: Development of the egg in the stick insect Didyuris violescens with particular reference to diapause. - Austral. J. Zool. 19: 141-158. Go to original source...
    20. Topp W. 1990: Selection for an optimal monovoltine life cycle in an unpredictable environment. Studies on the beetle Catops nigricans Spence (Col., Catopidae). - Oecologia 84: 134-141. Go to original source...
    21. Tzanakakis M.E. & Veerman A. 1994: Effect of temperature on the termination of diapause in the univoltine almond seed wasp Eurytoma amygdali. - Entomol. Exp. Appl. 70: 27-39. Go to original source...
    22. Ueda T. 1978: Geographic variation in the life cycle of Lestes sponsa. - Tombo 21: 27-37.
    23. Yamaguchi H. & Nakamura K. 2015: Effects of environmental factors on the regulation of egg diapause in the walking-stick insect, Ramulus irregulariterdentatus (Phasmatodea: Phasmatidae). - Eur. J. Entomol. 111: 35-40. Go to original source...
    24. Yano K., Ozaki T., Suzuki T., Yamazaki H., Nasuno M., Degawa Y. & Tojo K. 2021: Outbreak of the stick insect, Ramulus mikado (Phasmatodea, Phasmatidae), in the Akashina area of Japan (Azumino City, Nagano Prefecture). - Entomol. Sci. 24: 196-200. Go to original source...
    25. Yoder J.A. & Denlinger D.L. 1992: Water vapour uptake by diapausing eggs of a tropical walking stick. - Physiol. Entomol. 17: 97-103. Go to original source...
    26. Zar J.H. 2010: Biostatistical Analysis. 5th ed. Pearson Prentice Hall, Upper Saddle River, 944 pp.

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