Eur. J. Entomol. 118: 307-314, 2021 | DOI: 10.14411/eje.2021.031

Relationship between Rab and insulin-like proteins in the nervous system of Bombyx moriOriginal article

Tomohide UNO1, Yusuke OZAKIYA1, Mako SASAO1, Katsuhiko SAKAMOTO1, Yasuo YAMAUCHI2, Yuichi UNO3, Kengo KANAMARU1, Akira MIZOGUCHI4
1 Department of Applied Chemistry in Bioscience Biological Chemistry, Faculty of Agriculture, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan; e-mails: unotom@kobe-u.ac.jp, ksakamoto@diamond.kobe-u.ac.jp, kng@kobe-u.ac.jp
2 Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan; e-mail: yamauchi@kobe-u.ac.jp
3 Department of Plant Resource Science, Faculty of Agriculture, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan; e-mail: yuno@kobe-u.ac.jp
4 Division of Liberal Arts and Sciences, Aichi Gakuin University, Nisshin, Aichi 470-0195, Japan; e-mail: amizo@dpc.agu.ac.jp

Rab proteins are small GTP-binding proteins and are the largest family in the Ras GTPase superfamily and mediate vesicular transport in cells. Diverse insulin-like peptides, such as bombyxin, are synthesized in the brain and secreted into the haemolymph by the corpus allatum (CA). In the brain of Bombyx mori, Rabs are expressed in a specific area; however, which Rabs actually link the secretion of bombyxin remains unknown. A double-staining analysis of nine Rabs (Rab1, 3, 6, 7, 14, 21, 26, 39 and X4) and bombyxin indicated that Rab3-, Rab7-, Rab39- and RabX4-immunohistochemical reactivity (ir) areas overlapped with bombyxin-ir in the brain and CA in B. mori, while Rab6-, Rab14- and Rab21-irs partially overlapped in the CA. Rab1-ir occurred in the other immunopositive areas in CA. Rab26-ir did not occur in the brain. Rab39-ir occurred in UNC104, Rab39- effector, -immunopositive neurons in the brain and CA. Thus, Rab3, 7, 39 and X4 may regulate the exocytosis of bombyxin.

Keywords: Lepidoptera, Bombyx mori, larva, Rab proteins, bombyxin, brain, corpus allatum

Received: April 11, 2021; Revised: September 14, 2021; Accepted: September 14, 2021; Published online: October 7, 2021  Show citation

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UNO, T., OZAKIYA, Y., SASAO, M., SAKAMOTO, K., YAMAUCHI, Y., UNO, Y., KANAMARU, K., & MIZOGUCHI, A. (2021). Relationship between Rab and insulin-like proteins in the nervous system of Bombyx mori. EJE, 118, Article 307-314. https://doi.org/10.14411/eje.2021.031
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