Eur. J. Entomol. 118: 142-147, 2021 | DOI: 10.14411/eje.2021.015

Sublethal concentrations of spinosad synergize the pathogenicity of fungi to larvae of Ephestia kuehniella (Lepidoptera: Pyralidae)Original article

Fariba SOHRABI ORCID...1, Fatemeh JAMALI ORCID...1, J.P. MICHAUD ORCID...2
1 Department of Plant Protection, Faculty of Agriculture, Persian Gulf University, P.O. Box 75169-13798, Bushehr, Iran; e-mails: fsohrabi@pgu.ac.ir, jamali@pgu.ac.ir
2 Department of Entomology, Kansas State University, Agricultural Research Center-Hays, Hays, KS 67601, USA; e-mail: jpmi@ksu.edu

We evaluated the efficacy of four entomopathogenic fungi (EPF) and their compatibility with the bioinsecticide spinosad for control of Ephestia kuehniella (Zeller) under laboratory conditions. Three EPF, including Beauveria bassiana (Balsamo-Criveili) Vuillemin isolates Z1 and Iran 1395C, Lecanicillium (= Verticillium) lecanii (Zimmerman) Zare & Gams, isolate Iran 229, and Purpureocillium (Paecilomyces) lilacinum (Thom) Luangs-ard, Hywel-Jones & Samson, isolate Iran 1026 were tested against third and fifth larval instars of Ephestia kuehniella using a filter paper bioassay. Mortality caused by the EPF ranged from 63.3-72.5% for third instars and 50-65.5% for fifth instars, with LT50 ranging from 8.4-10.5 d and 10.1-12.9 d, respectively. The effect of spinosad at LC10 (= 26.2 ppm) on EPF spore germination was evaluated and found to be negligible, ranging from 0% for B. bassiana Z1 to 5.7% for P. lilacinum. The LC50 values for spinosad against third and fifth instar E. kuehniella larvae were 452.5 and 1446 ppm, respectively. Subsequently, spinosad at LC10 was applied to third instar E. kuehniella larvae 24 h before application of the EPFs at LC50. The addition of spinosad to applications of L. lecanii and B. bassiana Z1 and Iran1395C isolates synergized their pathogenicity to E. kuehniella larvae, whereas the effect was merely additive for P. lilacinum. Our results suggest that these EPF isolates can be used effectively in combination with spinosad for management of E. kuehniella in stored products.

Keywords: Lepidoptera, Pyralidae, Ephestia kuehniella, entomopathogenic fungi, Beauveria bassiana, Purpureocillium lilacinum, Lecanicillium lecanii, biopesticide, spinosad, stored products

Received: March 25, 2021; Revised: May 24, 2021; Accepted: May 24, 2021; Published online: June 1, 2021  Show citation

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SOHRABI, F., JAMALI, F., & MICHAUD, J.P. (2021). Sublethal concentrations of spinosad synergize the pathogenicity of fungi to larvae of Ephestia kuehniella (Lepidoptera: Pyralidae). EJE, 118, Article 142-147. https://doi.org/10.14411/eje.2021.015
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    References

    1. Abbott W.S. 1925: A method of computing the effectiveness of an insecticide. - J. Econ. Entomol. 18: 265-267. Go to original source...
    2. Asi M.R., Bashir M.H., Afzal M., Ashfaq M. & Sahi S.T. 2010: Compatibility of entomopathogenic fungi, Metarhizium anisopliae and Paecilomyces fumosoroseus with selective insecticides. - Pak. J. Bot. 42: 4207-4214.
    3. Athanassiou C.G., Rumbos C.I., Sakka M.K., Vayias B.J., Stephou V.K. & Nakas C.T. 2016: Insecticidal effect of the combined application of spinosad, Beauveria bassiana and diatomaceous earth for the control of Tribolium confusum. - Biocon. Sci. Tech. 26: 809-819. Go to original source...
    4. Athanassiou C.G., Kavallieratos N.G., Rumbos C.I., Stavropoulos D.J., Boukouvala M.C. & Nika E.P. 2018: Laboratory studies on the behavioral responses of Tribolium confusum and Ephestia kuehniella to surfaces treated with diatomaceous earth and spinosad formulations. - J. Pest Sci. 91: 299-311. Go to original source...
    5. Bahmani N., Ostovan H., Latifian M. & Rad B. 2012: Study on the lethal doses of suitable isolates of Beauveria bassiana for microbial control of Ephestia kuehniella on Sayer date cultivar. - Plant Prot. J. 4: 67-81.
    6. Bahmani N., Latifia M., Ostovan H. & Hesami S. 2020: Pathogenic effects of Beauveria bassiana and Bacillus thuringiensis on the population dynamics of Ephestia kuehniella. - Egyp. J. Biol. Pest Contr. 30: 1-9. Go to original source...
    7. Barra P., Rosso L., Nesci A. & Etcheverry M. 2013: Isolation and identification of entomopathogenic fungi and their evaluation against Tribolium confusum, Sitophilus zeamais, and Rhyzopertha dominica in stored maize. - J. Pest Sci. 86: 217-226. Go to original source...
    8. Batta Y.A. & Kavallieratos N.G. 2018: The use of entomopathogenic fungi for the control of stored-grain insects. - Intern. J. Pest Manag. 64: 77-87. Go to original source...
    9. Ben-Lalli A., Meot J.M., Collignan A. & Bohuon P. 2011: Modelling heat-disinfestation of dried fruits on "biological model" larvae, Ephestia kuehniella (Zeller). - Food Res. Intern. 44: 156-166. Go to original source...
    10. Buda V. & Peciulyte D. 2008: Pathogenicity of four fungal species to Indian meal moth Plodia interpunctella (Hubner) (Lepidoptera: Pyralidae). - Ekologija 54: 265-270. Go to original source...
    11. Draganova S. & Markova E. 2006: Bioassays with isolates of entomopathogenic fungi against Ephestia kuehniella (Zeller) (Lepidoptera: Pyralidae). - Bulg. J. Agric. Sci. 12: 637-643.
    12. Draganova S., Takov D. & Doychev D. 2007: Bioassays with isolates of Beauveria bassiana (Bals.) Vuill. and Paecilomyces farinosus (Holm.) Brown & Smith against Ips sexdentatus Boerner and Ips acuminatus Gyll. (Coleoptera: Scolytidae). - Plant Sci. 44: 24-28.
    13. Ericsson J.D., Todd Kabaluk J., Goettel M.S. & Myers J.H. 2007: Spinosad interacts synergistically with the insect pathogen Metarhizium anisopliae against the exotic wireworms Agriotes lineatus and Agriotes obscurus (Coleoptera: Elateridae). - J. Econ. Entomol. 100: 31-38. Go to original source...
    14. Erler F. & Ates A.O. 2015: Potential of two entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Coleoptera: Scarabaeidae), as biological control agents against the June beetle. - J. Insect Sci. 15(1): 44, 6 pp. Go to original source...
    15. Faraji S., Mehrvar A. & Shadmehri A.D. 2013: Studies on the virulence of different isolates of Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metcsn.) Sorokin against Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). - Afric. J. Agric. Res. 8: 4157-4161.
    16. Gabarty A., Salem H.M., Fouda M.A., Abas A.A. & Ibrahim A.A. 2014: Pathogencity induced by the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in Agrotis ipsilon. - J. Rad. Res. Appl. Sci. 7: 95-100. Go to original source...
    17. Gad H.A., Al-Anany M.S., Atta A.A.M. & Abdelgaleil S.A.M. 2021: Efficacy of low-dose combinations of diatomaceous earth, spinosad and Trichoderma harzianum for the control of Callosobruchus maculatus and Callosobruchus chinensis on stored cowpea seeds. - J. Stored Prod. Res. 91: 101778, 8 pp. Go to original source...
    18. Hertlein M.B., Thompson G.D., Subramanyam B. & Athanassiou C.G. 2011: Spinosad: a new natural product for stored grain protection. - J. Stored Prod. Res. 46: 151-166. Go to original source...
    19. Hokkanen H.M.T. & Kotiluoto R. 1992: Bioassay of the side effects of pesticides on Beauveria bassiana and Metarhizium anisopliae: standardized sequential testing procedure. - IOBC/WPRS Bull. 11: 148-151.
    20. Lynn D.E. & Ferkovich S.M. 2004: New cell lines from Ephestia kuehniella: characterization and susceptibility to baculoviruses. - J. Insect Sci. 4: 9-13. Go to original source...
    21. Machekano H., Mvumi B.M., Chinwada P., Richardson-Kageler, S.J. & Rwafa R. 2017: Efficacy of diatomaceous earths and their low-dose combinations with spinosad or deltamethrin against three beetle pests of stored-maize. - J. Stored Prod. Res. 72: 128-137. Go to original source...
    22. Machekano H., Mvumi B.M., Chinwada P., Richardson-Kageler, S.J. & Rwafa R. 2019: Evaluation of alternatives to synthetic pesticides under small-scale farmer-managed grain storage conditions. - Crop Prot. 126: 104941, 12 pp. Go to original source...
    23. Mahdavi V., Saber M., Rafiee-Dastjerdi H. & Kamita S.G. 2015: Lethal and demographic impact of chlorpyrifos and spinosad on the ectoparasitoid Habrobracon hebetor (Say) (Hymenoptera: Braconidae). - Neotrop. Entomol. 44: 626-633. Go to original source...
    24. Marcuzzo L.L. & Eli K. 2016: Effect of temperature and photoperiod on the in vitro germination of conidia of Botrytis squamosa, the causal agent of Botrytis leaf blight of onion. - Summa Phytopath. 42: 261-263. Go to original source...
    25. Mertz F.P. & Yao R.C. 1990: Saccharopolyspora spinosa sp. nov. isolated from soil collected in a sugar mill rum still. - Intern. J. Syst. Evol. Microbiol. 40: 34-39. Go to original source...
    26. Mollaie M., Izadi H. & Dashti H. 2011: Efficacy of spinosad against three stored-product insect pests. - Iran. J. Entomol. 1: 8-12.
    27. Mora M.A.E., Castilho A.M.C. & Fraga M.E. 2017: Classification and infection mechanism of entomopathogenic fungi. - Arq. Inst. Biol. 84: 1-10. Go to original source...
    28. Mutambuki K., Ngatia C.M., Mbugua J.N. & Likhayo P. 2003: Evaluation of the efficacy of spinosad dust against major storage pests. In Credland P.F., Armitage D.M., Bell C.H., Cogan P.M. & Highley E. (eds): Proceedings of the 8th International Working Conference on Stored Product Protection, 22-26 July 2002, York, UK. CAB International, Wallingford, pp. 888-891. Go to original source...
    29. Navon A. & Ascher K.R.S. 2000: International, Bioassays of Entomopathogenic Microbes and Nematodes. CABI, Wallinford, 336 pp. Go to original source...
    30. Nayak M.K. & Daglish G.J. 2017: Base-line susceptibility of field populations of Rhyzopertha dominica (F.) to spinosad in Australia. - J. Stored Prod. Res. 70: 1-6. Go to original source...
    31. Oliveira C.N.D., Neves P.M.O J. & Kawazoe L.S. 2003: Compatibility between the entomopathogenic fungus Beauveria bassiana and insecticides used in coffee plantations. - Sci. Agric. 60: 663-667. Go to original source...
    32. Paula A.R., Carolino A.T., Paula C.O. & Samuels R.I. 2011: The combination of the entomopathogenic fungus Metarhizium anisopliae with the insecticide imidacloprid increases virulence against the dengue vector Aedes aegypti (Diptera: Culicidae). - Paras. Vect. 4: 1-8. Go to original source...
    33. Pimentel M.A., Faroni L.R.D.A., da Silva F.H., Batista M.D. & Guedes R.N. 2010: Spread of phosphine resistance among Brazilian populations of three species of stored product insects. - Neotrop. Entomol. 39: 101-107. Go to original source...
    34. Pozidi-Metaxa E. & Athanassiou C.G. 2013: Comparison of spinosad with three traditional grain protectants against Prostephanus truncatus (Horn) and Ephestia kuehniella (Zeller) at different temperatures. - J. Pest Sci. 86: 203-210. Go to original source...
    35. Sabbour M., Abd-El-Aziz S. & Sherief M. 2012: Efficacy of three entomopathogenic fungi alone or in combination with diatomaceous earth modifications for the control of three pyralid moths in stored grains. - J. Plant Prot. Res. 52: 359-363. Go to original source...
    36. Sain S.K., Monga D., Kumar R., Nagrale D.T., Hiremani N.S. & Kranthi S. 2019: Compatibility of entomopathogenic fungi with insecticides and their efficacy for IPM of Bemisia tabaci in cotton. - J. Pest. Sci. 44: 97-105. Go to original source...
    37. SAS Institute 2003: The SAS System for Windows, Release 9.0., SAS, Institute. Cary, NC.
    38. Shakarami J., Eftekharifar R., Latifian M. & Jafari S. 2015: Insecticidal activity and synergistic effect of Beauvaria bassiana (Bals.) Vuill. and three botanical compounds against third instar larvae of Ephestia kuehniella Zeller. - Res. Crops 16: 296-303. Go to original source...
    39. Shishir A., Bhowmik A.A., Akanda N.R., Al Mamun A., Khan S.N. & Hoq M.M. 2015: Efficacy of indigenous Bacillus thuringiensis strains for controlling major vegetable pests in Bangladesh. - Egyp. J. Biol. Pest Con. 25: 729-734.
    40. da Silva R.A., Quintela E.D., Mascarin G.M., Barrigossi J.A.F. & Lião L.M. 2013: Compatibility of conventional agrochemicals used in rice crops with the entomopathogenic fungus Metarhizium anisopliae. - Sci. Agric. 70: 152-160. Go to original source...
    41. Snyder D.E., Meyer J., Zimmermann A.G., Qiao M., Gissendanner S.J., Cruthers L.R., Slone R.L. & Young D.R. 2007: Preliminary studies on the effectiveness of the novel pulicide, spinosad, for the treatment and control of fleas on dogs. - Veter. Parasitol. 150: 345-351. Go to original source...
    42. Sohrabi F., Jamali F., Morammazi S., Saber M. & Kamita S.G. 2019: Evaluation of the compatibility of entomopathogenic fungi and two botanical insecticides tondexir and palizin for controlling Galleria mellonella L. (Lepidoptera: Pyralidae). - Crop Prot. 117: 20-25. Go to original source...
    43. Subramanyam B., Boina D.R., Sehgal B. & Lazzari F. 2014: Efficacy of partial treatment of wheat with spinosad against Rhyzopertha dominica (F.) adults. - J. Stored Prod. Res. 59: 197-203. Go to original source...
    44. Talukder F. 2009: Pesticide resistance in stored-product insects and alternative biorational management: a brief review. - J. Agric. Marine Sci. 14: 9-15. Go to original source...

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