Eur. J. Entomol. 116: 432-441, 2019 | DOI: 10.14411/eje.2019.044

High-diversity microbiomes in the guts of bryophagous beetles (Coleoptera: Byrrhidae)Original article

Petr PYSZKO1, Martin ŠIGUT1,2, Martin KOSTOVČÍK3,4, Pavel DROZD1,*, Jiří HULCR2,5,6
1 Department of Biology and Ecology/Institute of Environmental Technologies, Faculty of Science, University of Ostrava, Chittussiho 10, 710 00 Ostrava, Czech Republic; e-mails: pyszko.petr@gmail.com, marton.sigut@gmail.com, pavel.drozd@osu.cz
2 Microbiology Institute, Academy of Science of the Czech Republic, Vídeňská 1083, 142 20 Praha 4, Czech Republic
3 BIOCEV, Institute of Microbiology, Academy of Sciences of the Czech Republic, Průmyslová 595, 252 42 Vestec, Czech Republic; e-mail: kostovci@gmail.com
4 Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Viničná 5, 128 44 Praha, Czech Republic
5 School of Forest Resources and Conservation, University of Florida - IFAS, 1745 McCarty Drive, Gainesville, FL 32611-0410, USA; e-mail: hulcr@ufl.edu
6 Entomology and Nematology Department, University of Florida - IFAS, 1881 Natural Area Drive, Gainesville, FL 32611, USA

The diversity and role of the gut microbiota of insects is a rapidly growing field of entomology, primarily fueled by new metagenomic techniques. Whereas endosymbionts in the guts of xylophagous or herbivorous insects are well studied, the microbiomes in moss-eating (bryophagous) insects remain uncharacterized. Using the Illumina MiSeq platform, we determined the composition of microbiomes in the gut, abdomen and on the body surface of two bryophagous species: Simplocaria semistriata (Fabricius, 1794) and Curimopsis paleata (Erichson, 1846) (Coleoptera: Byrrhidae). Gut microbiomes differed substantially from abdominal microbiomes in the same individuals, which indicates the need to separate them during dissection. Microbiomes in the gut and abdomen differed markedly from surface microbial assemblages. Gut microbiomes in bryophages had the highest MOTU richness, diversity and relative rarity. The eudominant bacteria in the guts and abdomens of bryophages were Novosphingobium, Bradyrhizobium, Ralstonia and Caulobacter, which are responsible for the detoxification of secondary metabolites or nitrogen fixation. These are less common in the surface samples and, therefore, likely to be associated with the specific ability of bryophages to feed on mosses.

Keywords: Coleoptera, Byrrhidae, bryophagy, gut microbiota, Illumina MiSeq, richness

Received: January 21, 2019; Accepted: August 22, 2019; Published online: November 26, 2019  Show citation

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PYSZKO, P., ŠIGUT, M., KOSTOVČÍK, M., DROZD, P., & HULCR, J. (2019). High-diversity microbiomes in the guts of bryophagous beetles (Coleoptera: Byrrhidae). EJE, 116, Article 432-441. https://doi.org/10.14411/eje.2019.044
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