EUROPEAN JOURNAL OF ENTOMOLOGY

Eur. J. Entomol. 116: 258-268, 2019 | DOI: 10.14411/eje.2019.030

Identification of vibrational signals emitted by embryos of the migratory locust Locusta migratoria (Orthoptera: Acrididae) that induce synchronous hatchingOriginal article

Hironori SAKAMOTO^1,2, Seiji TANAKA^3,4,*, Tamako HATA⁵

¹ Organization for University Research Initiatives, Waseda University, Shinjuku-ku, Tokyo 162-8480, Japan; e-mail: hirowol@gmail.com

² Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, Onogawa 16-2, Tsukuba, Ibaraki 305-0053, Japan

³ National Institute of Agro-biological Sciences at Ohwashi (NIASO), Tsukuba, Ibaraki 305-8634, Japan; e-mail: stanaka117@yahoo.co.jp

⁴ Matsushiro 1-20-19, Tsukuba, Ibaraki 305-0035, Japan

⁵ National Agriculture and Food Research Organization (NARO), Ohwashi 1-2, Tsukuba, Ibaraki 305-8634, Japan; e-mail: tamako@affrc.go.jp

Eggs of the migratory locust, Locusta migratoria (Orthoptera: Acrididae), hatch synchronously when in a pod, but only sporadically when kept separately. Here, we aimed to detect the vibrational stimuli emitted by eggs that initiate synchronous hatching. First, we recorded the vibrations emitted by an egg pod and single eggs. One bout of vibrations consisted of 2 to 46 vibrations. The total number and amplitude of vibrations in single eggs increased as the time to hatch decreased. Eggs kept separately were continuously subjected during the last 2 days before hatching to recordings of vibrations from a single egg. Recordings made during the last 2.5 h before hatching caused these eggs to hatch significantly earlier than those not subjected to this treatment, the control. In contrast, eggs subjected to recordings made 8 to 10 h before hatching significantly delayed their hatching relative to the controls, which indicates that synchronous hatching of eggs is induced by age-dependent changes in vibrations from neighbouring eggs. Exposure to one large bout of vibrations (consisting of 40 vibrations in 101 s) was sufficient to induce synchronous hatching in eggs kept separately when applied 5 h before hatching, but not 36 or 11.5 h before hatching. Visual inspection of the spectra indicated that the vibrations had two peaks at about 100 Hz and 1.5 kHz. Only exposure to the latter altered the hatching time of eggs. The embryo moved the posterior part of its abdomen when emitting the vibrations. These results indicate that the synchronous hatching of locust eggs is induced when the embryos emit particular vibrations.

Keywords: Orthoptera, Acrididae, Locusta migratoria, embryo-embryo interaction, hatching synchrony, vibration

Received: April 2, 2019; Revised: August 1, 2019; Accepted: August 1, 2019; Published online: August 22, 2019  Show citation