Eur. J. Entomol. 116: 1-9, 2019 | DOI: 10.14411/eje.2019.001

Effects of temperature on the activity of antioxidant enzymes in larvae of Bactrocera dorsalis (Diptera: Tephritidae) parasitized by Diachasmimorpha longicaudata (Hymenoptera: Braconidae): Optimizing the mass rearing of this braconid by varying the temperatureOriginal article

Pumo CAI1,2,3, Yong WANG1,2,3, Chuandong YI1,2,3, Qiwen ZHANG1,2,3, Huimin XIA1,2,3, Jia LIN1,2,3, Hehe ZHANG1,2,3, Jianquan YANG1,2,3, Qinge JI1,2,3,*, Jiahua CHEN1,2,3
1 Institute of Beneficial Insects, Plant Protection College, Fujian Agriculture and Forestry University, 350002 Fuzhou, P.R. China; e-mails: caipumo@qq.com, 1506293481@qq.com, 602328593@qq.com, 344463987@qq.com, 523441201@qq.com, 776954169@qq.com, zhh680216@163.com, 2576680960@qq.com, jiqinge@yeah.net, jhchen34@yeah.net
2 State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, 350002 Fuzhou, P.R. China
3 Key Lab of Biopesticide and Chemical Biology, Ministry of Education, 350002 Fuzhou, P.R. China

Diachasmimorpha longicaudata is the most widely used endoparasitoid in biocontrol programmes against various species of tephritids and is continuously mass-reared under laboratory conditions at a constant temperature; however, little is known about how varying the temperature might affect the effectiveness of the mass rearing of immature D. longicaudata. This study aimed to determine the optimum temperature for the development of larvae D. longicaudata the larvae of its host, Bactrocera dorsalis. Third-instar B. dorsalis were exposed for 4 h to five pairs of parasitoids, and then they were kept at one of six temperatures ranging from 19°C to 34°C. The activities of three major antioxidant enzymes (CAT, POX, SOD) were individually measured 4, 24, 48 and 72 h after exposure to each of the six temperatures. The results were as follows: for CAT the highest CAT activities were recorded at 25°C (intervals 5-24 h and 25-48 h), at 28°C (interval 0-4 h) and at 31°C (interval 49-72 h), and for POX and SOD identically at 25°C (intervals 0-4 h, 5-24 h) and at 31°C (intervals 25-48 h, 49-72 h), respectively. Subsequently, the effects keeping the parasitized larvae at these different temperatures on the biological attributes of D. longicaudata reared under laboratory conditions were investigated. The percentage of their eggs that hatched, percentage of adults that emerged, pupal weight and longevity were significantly greater than that recorded for the control when the parasitized host larvae were kept at 28°C for 4 h, 25°C for 5 and 48 h and 31°C for 49 and 72 h.

Keywords: Diptera, Tephritidae, Bactrocera dorsalis, Hymenoptera, Braconidae, Diachasmimorpha longicaudata, parasitoid, altering temperatures, mass-reared, activity of antioxidants

Received: February 23, 2018; Revised: November 20, 2018; Accepted: November 20, 2018; Published online: January 4, 2019  Show citation

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CAI, P., WANG, Y., Chuandong, Y., ZHANG, Q., XIA, H., LIN, J., ... CHEN, J. (2019). Effects of temperature on the activity of antioxidant enzymes in larvae of Bactrocera dorsalis (Diptera: Tephritidae) parasitized by Diachasmimorpha longicaudata (Hymenoptera: Braconidae): Optimizing the mass rearing of this braconid by varying the temperature. EJE, 116, Article 1-9. https://doi.org/10.14411/eje.2019.001
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