Eur. J. Entomol. 115: 264-267, 2018 | DOI: 10.14411/eje.2018.026

Isolation and characterization of 15 microsatellite markers for the highly invasive box tree moth Cydalima perspectalis (Lepidoptera: Crambidae)Note

Audrey BRAS1, Laure SAUNÉ2, Alain ROQUES1, Jérôme ROUSSELET1, Marie-Anne AUGER-ROZENBERG1
1 INRA, UR633 Unité de Recherche de Zoologie Forestière, 2163 Avenue de la Pomme de Pin, CS 40001 ARDON 45075 ORLEANS Cedex 2, France; e-mails: audrey.bras@inra.fr, alain.roques@inra.fr, jerome.rousselet@inra.fr, marie-anne.auger-rozenberg@inra.fr
2 UMR1062 CBGP (INRA, CIRAD, IRD, Montpellier Supagro), INRA, 755 avenue du campus Agropolis, CS 30016, 34988 Montferrier-sur-lez Cedex, France; e-mail: laure.saune@inra.fr

In this study, we report the development of a set of 15 polymorphic microsatellite markers for the box tree moth, Cydalima perspectalis (Walker), a highly invasive insect in Europe causing significant damage to natural and ornamental Buxus trees. The markers were characterized for four distant populations in both its native (China, two populations) and invasive ranges (Czech Republic and Turkey, one population each). The number of alleles ranged from 2 to 12. No marker significantly deviated from the Hardy-Weinberg equilibrium for all the populations sampled. These microsatellite markers are promising tools for further studies on the invasive pathways and dispersal pattern of the box tree moth in Europe.

Keywords: Lepidoptera, Crambidae, Cydalima perspectalis, microsatellites, invasive insect, genetic diversity

Received: February 2, 2018; Revised: May 16, 2018; Accepted: May 16, 2018; Published online: June 6, 2018  Show citation

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BRAS, A., SAUNÉ, L., ROQUES, A., ROUSSELET, J., & AUGER-ROZENBERG, M. (2018). Isolation and characterization of 15 microsatellite markers for the highly invasive box tree moth Cydalima perspectalis (Lepidoptera: Crambidae). EJE, 115, Article 264-267. https://doi.org/10.14411/eje.2018.026
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