Eur. J. Entomol. 104 (4): 641-645, 2007 | DOI: 10.14411/eje.2007.080

Monoclonal antibody against Rab8 from Bombyx mori (Lepidoptera: Bombycidae)

Tomohide UNO1, Takuya NAKADA1, Yuichi UNO2, Kengo KANAMARU1, Hiroshi YAMAGATA1, Masahiko NAKAMURA3, Michihiro TAKAGI4
1 Laboratory of Biological Chemistry, Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Nada-ku, Hyogo 657-8501, Japan; e-mail: unotom@kobe-u.ac.jp
2 Department of Plant Resource Science, Faculty of Agriculture, Kobe University, Nada-ku, Hyogo 657-8501, Japan
3 Department of Bioscience and Biotechnology, Faculty of Bioenvironmental Science, Kyoto Gakuen University, 1-1 Nanjo, Sogabe, Kameoka, Kyoto 621-8555, Japan
4 Department of Animal Science, Faculty of Agriculture, Kobe University, Nada-ku, Hyogo 657-8501, Japan

Small GTPases of the Rab family are key regulators of membrane trafficking. Monoclonal antibodies are useful tools for identifying proteins that interact with other proteins and for examining their tissue distribution. We selected a monoclonal antibody against Rab8 of Bombyx mori L. It specifically recognized amino acid residues 30-109, which are conserved among Rab8 proteins, and did not recognize any other Rab proteins. Western blotting using the antibody revealed one band in the brains of B. mori and rat. Far-Western blotting analysis detected three proteins interacting with Rab8. These results indicate that this antibody is useful for clarifying the physiological function of Rab8 of B. mori and other species. This is a report of a study on a monoclonal antibody against insect Rab protein.

Keywords: Bombyx mori, monoclonal antibody, Rab proteins, brain

Received: March 22, 2007; Revised: June 7, 2007; Accepted: June 7, 2007; Published: October 15, 2007  Show citation

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UNO, T., NAKADA, T., UNO, Y., KANAMARU, K., YAMAGATA, H., NAKAMURA, M., & TAKAGI, M. (2007). Monoclonal antibody against Rab8 from Bombyx mori (Lepidoptera: Bombycidae). EJE, 104(4), 641-645. doi: 10.14411/eje.2007.080
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