Eur. J. Entomol. 101 (4): 503-511, 2004 | DOI: 10.14411/eje.2004.071

Phylogeny of Iberian Zabrus (Coleoptera: Carabidae: Zabrini) based on mitochondrial DNA sequence

José-Fermín SÁNCHEZ-GEA, José GALIÁN, José SERRANO*
Departamento de Zoología, Facultad de Veterinaria, Universidad de Murcia, Campus de Espinardo, Aptdo. 4021, 30071 Murcia, Spain; e-mails: jfermin@um.es; jgalian@um.es; jserrano@um.es

The genus Zabrus Clairville, 1806 is a Holomediterranean taxon that radiated into about a hundred species most likely during the Cenozoic. There are four endemic subgenera on the Iberian Peninsula, which include 28 species, Epomidozabrus (3 spp.), Euryzabrus (1 sp.), Platyzabrus (2 spp.) and Iberozabrus (22 spp.). A mitochondrial fragment comprising part of the cytochrome-oxidase-I, tRNAleu, and part of the cytochrome-oxidase-II genes was sequenced of most of these species. Taxa of other subgenera of Zabrus (Macarozabrus, Polysitus, Zabrus, and Pelor), six species of Amara (the putative sister taxon of Zabrus), and representatives of tribes related to Zabrini were also sequenced. The results show that the genus Zabrus is a monophyletic taxon well separated from Amara. The four subgenera endemic to the Iberian Peninsula also make up a monophyletic clade, which stresses the association between the geographic distribution and the monophyly of many supraspecific taxa within this genus. The species-rich subgenus Iberozabrus seems to be the sister taxon of the clade made up of the three other subgenera endemic to the Iberian Peninsula. The Iberozabrus species-groups proposed by Andújar & Serrano in 2001, based on morphological and geographical grounds, are only partly corroborated by the molecular phylogeny. The lack of congruence between these data sets is mainly between those species-groups with large numbers of species and more complex geographic patterns. Some cases of incongruence are possibly due to homoplasic external characters appeared by convergent evolution.

Keywords: Molecular phylogeny, mitochondrial COI sequence, karyotype analysis, Zabrus, Carabidae, Coleoptera

Received: April 1, 2004; Revised: July 26, 2004; Accepted: August 31, 2004; Published: December 20, 2004  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
SÁNCHEZ-GEA, J., GALIÁN, J., & SERRANO, J. (2004). Phylogeny of Iberian Zabrus (Coleoptera: Carabidae: Zabrini) based on mitochondrial DNA sequence. EJE, 101(4), 503-511. doi: 10.14411/eje.2004.071
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. ANDUJAR A. & SERRANO J. 2000: Taxonomic notes on the genus Zabrus (Coleoptera, Carabidae, Zabrini). Boln. Asoc. Esp. Entomol. 24: 185-193
    2. ANDUJAR A. & SERRANO J. 2001: Revision y filogenia de los Zabrus Clairville, 1806 de la peninsula Iberica (Coleoptera, Carabidae). Monografias de la Sociedad Aragonesa de Entomologia, no. 5, Zaragoza, 90 pp
    3. AVISE J.C. 2000: Phylogeography. The History and Formation of Species. Harvard University Press, Cambridge, 447 pp Go to original source...
    4. EMERSON B.R., OROMI P. & HEWITT G.M. 1999: MtDNA phylogeography and recent intra-island diversification among Canary Island Calathus beetles. Mol. Phyl. Evol. 13: 149-158 Go to original source...
    5. EMERSON B., OROMI P. & HEWITT G. 2000: Interpreting colonization of the Calathus (Coleoptera: Carabidae) of the Canary Islands and Madeira through the application of the parametric bootstrap. Evolution 54: 2081-2090 Go to original source...
    6. FREUDE H. 1986: Revision der zur (?) Gattung "Zabrus" Clairville, 1806 gehoerenden Arten mit Bestimmungstabellen (Col. Carabidae). Atti Mus. Civ. Stor. Nat. Trieste 39: 133-150
    7. FREUDE H. 1988: Revision der zur (?) Gattung "Zabrus" Clairville, 1806 gehoerenden Arten mit Bestimmungstabellen (Col. Carabidae). 2. Teil. Atti Mus. Civ. Stor. Nat. Trieste 41: 133-142
    8. FREUDE H. 1989: Revision der zur (?) Gattung "Zabrus" Clairville, 1806 gehoerenden Arten mit Bestimmungstabellen (Col. Carabidae). 3. Teil. Atti Mus. Civ. Stor. Nat. Trieste 42: 71-153
    9. FREUDE H. 1990: Revision der zur (?) Gattung "Zabrus" Clairville 1806 gehoerenden Arten mit Bestimmungstabellen (Col. Carabidae). 4. Atti Mus. Civ. Stor. Nat. Trieste 43: 329-364
    10. GALIAN J., ORTIZ A.S. & SERRANO J. 1991: Amara and Zabrus, two different patterns of karyotypic evolution (Coleoptera, Carabidae). Caryologia 44: 75-84 Go to original source...
    11. GALIAN J., DE LA RUA P., SERRANO J., JUAN C. & HEWITT G.M. 1999: Phylogenetic relationships in Iberian Scaritina (Coleoptera: Carabidae) inferred from mitochondrial COI sequences and karyotype analysis. J. Zool. Syst. Evol. Res. 37: 85- 92 Go to original source...
    12. GANGLBAUER L. 1915: Revision der Gattung Zabrus Clairv. Muench. Koleopt. Z. 4: 87-137
    13. HOWLAND D.E. & HEWITT G.M. 1995: Phylogeny of the Coleoptera based on mitochondrial cytocrome oxidase I sequence data. Insect Mol. Biol. 4: 203-215 Go to original source...
    14. JEANNE C. 1968: Carabiques de la Peninsule Iberique (9eme note). Act. Soc. Linn. Bordeaux (A) 105(8): 1-22
    15. KUMAR S., TAMURA K., JAKOBSEN I.B. & NEI M. 2001: MEGA2: Molecular Evolutionary Genetics Analysis software. Bioinformatics 17: 1244-1245 Go to original source...
    16. MACHADO A. 1992: Monografia de los carabidos de las Islas Canarias (Insecta, Coleoptera). Instituto de Estudios Canarios, La Laguna, 734 pp
    17. MADDISON W.P. & MADDISON D.R. 1992: MacClade. Analysis of Phylogeny and Character Evolution, v.3. Sinauer Associates Inc., Sunderland, 398 pp. + computer disk
    18. MARTINEZ-NAVARRO E.M., GALIAN J. & SERRANO J. (in press): Phylogeny and molecular evolution of the tribe Harpalini (Coleoptera, Carabidae) inferred from mitochondrial cytochrome-oxidase I. Mol. Phyl. Evol
    19. NOVOA F., MARINO J. & LOMBARDERO J. 1996: Los Carabidae (Coleoptera) de los montes del Invernadeiro (NO. Peninsula Iberica) y algunas consideraciones sobre la carabidofauna de las Montanas Orientales de Galicia. Bol. R. Soc. Esp. Hist. Nat. (Biol.) 92: 167-175
    20. OOSTERBROEK P. & ARNTZEN J.W. 1992: Area-cladograms of Circum-Mediterranean taxa in relation to Mediterranean paleogeography. J. Biogeogr. 19: 3-20 Go to original source...
    21. ORTUNO V.M., SERRANO J., ANDUJAR A. & LENCINA J.L. 2003: The female genitalia of the genus Zabrus (Coleoptera: Carabidae: Zabrini). I. The general structure and the subgenera Zabrus, Euryzabrus, Platyzabrus and Epomidozabrus. Eur. J. Entomol. 94: 115-121 Go to original source...
    22. POSADA D. & CRANDALL K.A. 1998: Modeltest: testing the model of DNA substitution. Bioinformatics 14: 817-818 Go to original source...
    23. SAMBROOK J., FRITSCH E.F. & MANIATIS T. 1989: Molecular Cloning: A Laboratory Manual. Vol. 3. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
    24. SANCHEZ-GEA J.F., SERRANO J. & GALIAN J. 2000: Variability in rDNA loci in Iberian species of the genus Zabrus (Coleoptera: Carabidae) detected by fluorescence in situ hybridization. Genome 43: 22-28 Go to original source...
    25. SIMON C., FRATI F., BECKENBACH A., CRESPI B., LIU H. & FLOOK P. 1994: Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann. Entomol. Soc. America 87: 651-701 Go to original source...
    26. SWOFFORD D.L., OLSEN G.L., WADDELL P.J. & HILLIS D.M. 1996: Phylogenetic Inference. In Hillis D.M., Moritz C. & Mable B.K. (eds): Molecular Systematics. 2nd ed. Sinauer Associates, Sunderland, pp. 407-514
    27. THOMPSON J.D., HIGGINS D.G. & GIBSON T.J. 1994: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucl. Acids Res. 22: 4673-4680 Go to original source...
    28. WALSH P.S., METZQER D.A. & HIGUCHI R. 1991: Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10: 506-512

    This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content