EJE, vol. 106 (2009), issue 1

Chromosomal differentiation among bisexual European species of Saga (Orthoptera: Tettigoniidae: Saginae) detected by both classical and molecular methods


Eur. J. Entomol. 106 (1): 1-9, 2009 | 10.14411/eje.2009.001

We report the karyotype characteristics including chromosome numbers of Saga campbelli campbelli, S. c. gracilis, and S. rammei using the following classical cytogenetic methods: C-banding, silver staining, and fluorochrome staining DAPI and CMA3. We also present FISH data showing the distribution of telomeric repeats and 18S rDNA on the chromosomes of these species and the results of similar studies cited in the literature on S. hellenica, S. natoliae, and S. rhodiensis. The five European Saga species exhibit a high rate of karyotype evolution. In addition to changes in chromosome number...

Loss of genetic diversity through spontaneous colonization in the bog fritillary butterfly, Proclossiana eunomia (Lepidoptera: Nymphalidae) in the Czech Republic

Gabriel NÈVE, Alois PAVL═╚KO, Martin KONVI╚KA

Eur. J. Entomol. 106 (1): 11-19, 2009 | 10.14411/eje.2009.002

The butterfly Proclossiana eunomia (Lepidoptera: Nymphalidae) was discovered at a single locality in the Czech Republic in 1963. Until the 1980s, it was known from a restricted area, from which it spontaneously expanded during the 1980s to other localities up to 23 km from the source population. Samples were collected in 2002 from the source and ten other populations, totalling 274 specimens. All samples were analysed by electrophoresis for four polymorphic loci. Mean heterozygosity decreased with distance from the source population; this suggested a process of stepping stone colonization, involving the loss of rare alleles along the way. The...

Laboratory study of the effects of leek lectin (APA) in transgenic tobacco plants on the development of cotton leafworm Spodoptera littoralis (Lepidoptera: Noctuidae)


Eur. J. Entomol. 106 (1): 21-28, 2009 | 10.14411/eje.2009.003

Transgenic tobacco plants expressing the Allium porrum L. (leek) agglutinin (called APA) under the control of the 35S constitutive promoter were tested for their insecticidal activity against the cotton leafworm, Spodoptera littoralis (Lepidoptera: Noctuidae). Southern blot and PCR analysis confirmed that the APA gene was integrated into the plant genome. Northern and Western blots as well as semi-quantitative agglutination assays revealed lectin expression at various levels in the transgenic lines. Biochemical analyses indicated that the recombinant APA has the same molecular structure as the native lectin. Native and recombinant lectin...

Proteomic analysis of pre-diapause, diapause and post-diapause larvae of the wheat blossom midge, Sitodiplosis mosellana (Diptera: Cecidomyiidae)

Wei-Ning CHENG, Xiu-Lian LI, Feng YU, Yi-Ping LI, Jian-Jun LI, Jun-Xiang WU

Eur. J. Entomol. 106 (1): 29-35, 2009 | 10.14411/eje.2009.004

To determine the relationship between protein expression and insect diapause, a proteomic approach was used to investigate the proteins extracted from larvae of the wheat blossom midge Sitodiplosis mosellana Gehin at different developmental stages, including pre-diapause, over-summering diapause, over-wintering diapause and post-diapause. Using 2-DE gels stained with coomassie brilliant blue, about 300 protein spots were detected in the extracts of pre-diapause larvae and 275 for those in each of the other stages. There were 91, 92 and 95 protein spots that showed more than a 2-fold change in abundance in the over-summering diapause, over-wintering...

Sexual dimorphism in winter survival rate differs little between damselbug species (Heteroptera: Nabidae)


Eur. J. Entomol. 106 (1): 37-41, 2009 | 10.14411/eje.2009.005

The winter survival of three closely related univoltine heteropterans that overwinter as adults, Nabis rugosus, N. ericetorum and N. pseudoferus was investigated. After 150 days of low temperature treatment (3-5░C, 16L : 8D, r.h. 70-80%) more males than females died. The mortality rate ranged from 88.8 to 93.8% in males and from 54.2 to 60.7% in females. However, these sexual differences in mortality did not differ significantly across the three species and populations of different geographic origin. It remains a general question whether this considerable evolutionary stability in the sex-specific investment into reproduction...

Responses of life-history traits of brackish- and freshwater populations of the water strider to NaCl Aquarius paludum (Hemiptera: Gerridae)


Eur. J. Entomol. 106 (1): 43-48, 2009 | 10.14411/eje.2009.006

The water strider Aquarius paludum inhabits the surfaces of a wide variety of freshwater habitats both temporary and permanent. It can also live on the surface brackish-water around river mouths. Exposure to a brackish rather than freshwater environment may affect a range of adult and offspring life-history traits. In a two-stage experiment A. paludum offspring from fresh- (F) and brackish-water (B) populations were obtained and their offspring reared in either a fresh- or brackish-water (0.45% NaCl) environments. The four offspring treatment groups (F-F, F-B, B-F, B-B) varied in terms of the parental and offspring rearing environments....

Distribution and diversity of Wolbachia in different populations of the wheat aphid Sitobion miscanthi (Hemiptera: Aphididae) in China

Zhe WANG, Zuo-Rui SHEN, Yue SONG, Hong-Yue LIU, Zheng-Xi LI

Eur. J. Entomol. 106 (1): 49-55, 2009 | 10.14411/eje.2009.007

Wolbachia is a widely distributed intracellular symbiont in the reproductive tissues of arthropods. The wheat aphid Sitobion miscanthi (Takahashi) is an important agricultural pest worldwide. Wolbachia was detected in different populations of S. miscanthi in China using 16s rDNA and wsp-specific primers. Of eighteen populations eleven were infected with Wolbachia. Several strains of Wolbachia infected these S. miscanthi populations. Of the eleven infected populations, four were infected with only one Wolbachia strain and seven with double infections. This is the most systematic survey...

H.F. van Emden & R. Harrington (eds): Aphids as Crop Pests.


Eur. J. Entomol. 106 (1): 56, 2009 | 10.14411/eje.2009.008

CABI Publishing, London, 2007, 717 pp. ISBN 978 0 85199 819 0. Price USD 290.00 / EUR 230.00 / GBP 147.00.

Male flies with yellow balls - New observations on the eversible vesicles on the postabdomen of male Thaumatomyia notata (Diptera: Chloropidae)


Eur. J. Entomol. 106 (1): 57-62, 2009 | 10.14411/eje.2009.009

The paired eversible vesicles on the postabdomen of male T. notata are likely to play an important role in intraspecific communication and possibly in the notorious aggregation behaviour of these flies at buildings in autumn. The morphological and histological study of these organs provides new evidence supporting the hypothesis that they are pheromone glands. They also constitute a strong optical signal. A detailed survey of these structures could provide the bases for targeted control methods.

Use of volatiles of Aristolochia chilensis (Aristolochiaceae) in host searching by fourth-instar larvae and adults of Battus polydamas archidamas (Lepidoptera: Papilionidae: Troidini)

Carlos F. PINTO, Alejandra J. TRONCOSO, Alejandro URZ┌A, Hermann M. NIEMEYER

Eur. J. Entomol. 106 (1): 63-68, 2009 | 10.14411/eje.2009.010

Papilionid butterflies of the tribe Troidini are specialists on plants of the family Aristolochiaceae. The role of plant volatiles in host recognition by adult and larval stages of these insects remains unknown. We used Battus polydamas archidamas (Papilionidae: Troidini) and its host-plant, Aristolochia chilensis (Aristolochiaceae), to study: (i) the olfactory and electrophysiological responses of adults to headspace volatiles of the host-plant, (ii) the chemical composition of the headspace volatiles of the host-plant, (iii) the patterns of aggregation of larvae in the field in order to ascertain the time when they leave the plant where...

Combined effect of environmental factors on distribution of Geometridae (Lepidoptera) in South Korea

Sei-Woong CHOI, Jung-Hwa CHUN

Eur. J. Entomol. 106 (1): 69-76, 2009 | 10.14411/eje.2009.011

We analyzed patterns of geometrid species richness in South Korea to assess the observed and estimated species richness gradient, to determine predictors for the distribution of moths and to investigate the effect of latitude on the species richness of moths in the Korean peninsula. The species richness was analyzed for 541 geometrid moths across 17 quadrates, with each quadrate defined by one degree of latitude and longitude. Two subgroups of geometrid moths were adopted according to their distributional ranges: Palearctic (435 species, 80.4%) and Oriental (106 species, 19.6%). To investigate the relationship between the distribution of geometrid...

Distribution modelling as an approach to the conservation of a threatened alpine endemic butterfly (Lepidoptera: Satyridae)


Eur. J. Entomol. 106 (1): 77-84, 2009 | 10.14411/eje.2009.012

Mountain butterfly species are often restricted in their distribution and under threat from habitat destruction and climate change. Due to the inaccessibility of their habitats the distributions of many such species are unknown. We have investigated whether information on the habitat requirements of the Alpine endemic species Erebia calcaria could be used for modelling its potential distribution. We surveyed part of its range using transects and recorded habitat and environmental parameters. The most important parameters determining the presence of the species were average height of the vegetation, maximum height of the vegetation, percentage...

Seasonal occurrence and local coexistence within scarabaeid dung beetle guilds (Coleoptera: Scarabaeoidea) in Tunisian pasture


Eur. J. Entomol. 106 (1): 85-94, 2009 | 10.14411/eje.2009.013

Dung beetle assemblages were monitored using baited pitfall traps from January to December 2006 in Northern Tunisia. 4,965 beetles belonging to 37 species were trapped. Aphodius lineolatus and Onthophagus taurus dominated the assemblages. Results showed a significant seasonal variation in assemblage composition, and diversity. There were four periods of activity during the course of the year. Temporal turnover was highest in October and in February. Temporal distribution of species shows seasonal segregation and opposite patterns in the two dominant guilds (Aphodiinae-dwellers and Scarabaeidae-tunnelers). Aphodiidae-dwellers were active...

Afromontane Coelocorynus (Coleoptera: Scarabaeidae: Cetoniinae): Larval descriptions, biological notes and phylogenetic placement

Petr ę═PEK, Bruce D. GILL, Vasily V. GREBENNIKOV

Eur. J. Entomol. 106 (1): 95-106, 2009 | 10.14411/eje.2009.014

This paper reports the collecting of adult beetles and third-instar larvae of Coelocorynus desfontainei Antoine, 1999 in Cameroon and provides new data on the biology of this high-altitude Afromontane genus. It also presents the first diagnosis of this genus based on larval characters and examination of its systematic position in a phylogenetic context using 78 parsimony informative larval and adult characters. Based on the results of our analysis we (1) support the hypothesis that the tribe Trichiini is paraphyletic with respect to both Valgini and the rest of the Cetoniinae, and (2) propose that the Trichiini subtribe Cryptodontina,...

A review of the European species of Phradis (Hymenoptera: Ichneumonidae: Tersilochinae), with a description of a new species from Spain

Andrey I. KHALAIM, Santiago BORDERA, Alexander RODR═GUEZ-BERR═O

Eur. J. Entomol. 106 (1): 107-118, 2009 | 10.14411/eje.2009.015

European species of the genus Phradis F÷rster, 1869 are reviewed. A new species from Spain, Phradis mediterraneus sp. n., is described and illustrated. A key to all 24 European species and data on distribution and biology of these species are provided.

A review of the genus Zavrelia (Diptera: Chironomidae)

Torbjørn EKREM, Elisabeth STUR

Eur. J. Entomol. 106 (1): 119-144, 2009 | 10.14411/eje.2009.016

In this paper we review the taxonomy of the genus Zavrelia Kieffer, Thienemann & Bause and present emended generic diagnoses of all major life stages. Illustrated keys to larvae, pupae, adult males and females are presented as well as descriptions of four species new to science. Zavrelia species are only recorded from the northern hemisphere and comprise in total ten small to minute species. The following life stages and species are described: Larva, pupa, adult male and adult female of Zavrelia aristata sp. n., Zavrelia hudsoni sp. n., Zavrelia pentatoma Kieffer & Bause and Zavrelia sinica sp. n.;...

Tackling the "Protoblattoidea problem": Revision of Protoblattinopsis stubblefieldi (Dictyoptera; Late Carboniferous)

Olivier B╔THOUX, Klaus-Dieter KLASS, J÷rg W. SCHNEIDER

Eur. J. Entomol. 106 (1): 145-152, 2009 | 10.14411/eje.2009.017

The forewing morphology of the fossil species †Protoblattinopsis stubblefieldi Laurentiaux, 1953 (Dictyoptera; Late Carboniferous) is re-investigated. Unlike previously stated, in this species, the stems of R and M are distinct and CuA has branches. The occurrence of the putative plesiomorphies "long ScP" and "differentiated R sectors" (i.e., RA and RP) in †P. stubblefieldi indicates a position basal to all extant Dictyoptera and many members of its paraphyletic stem. Unlike representatives of the families †Archimylacridae and †Blattinopsidae, but similar to Blattodea s. str., †P. stubblefieldi...