EJE, vol. 105 (2008), issue 4

Limited variation in mitochondrial DNA of maize-associated Ostrinia nubilalis (Lepidoptera: Crambidae) in Russia, Turkey and Slovenia

Sugihiko HOSHIZAKI, Ryôta WASHIMORI, Shun-Ichi KUBOTA, Andrei N. FROLOV, Daisuke KAGEYAMA, Stanislav GOMBOC, Suguru OHNO, Sadahiro TATSUKI, Yukio ISHIKAWA

Eur. J. Entomol. 105 (4): 545-552, 2008 | 10.14411/eje.2008.073

A sequence analysis of the mitochondrial cytochrome oxidase subunit II (COII) gene in Russian and Turkish maize-associated populations of Ostrinia nubilalis and a Slovenian population of O. nubilalis probably infesting maize revealed little diversity. This lack of diversity may have resulted from bottleneck event(s) when the maize-associated population of O. nubilalis expanded from small population(s) in association with the cultivation of maize in Europe ca. 500 years ago. In the genealogy of COII genes obtained in the present and previous studies, Eurasian samples were substantially differentiated from North American samples....

The genetic architecture of extended life span in the seed beetle Acanthoscelides obtectus (Coleoptera: Bruchidae)

Darka ©E©LIJA, Nikola TUCIÆ

Eur. J. Entomol. 105 (4): 553-560, 2008 | 10.14411/eje.2008.074

We studied the genetic architecture of the differences in the longevity between lines selected for postponed senescence and a control population of the seed beetle Acanthoscelides obtectus maintained on two hosts. By using lines with increased longevity, which were obtained by selection on natural variation in longevity, we showed that the genetic architecture of seed beetle longevity is complex, with sex-specific effects and variation attributable to many interacting genes, whose expression depend on the host on which the beetles were reared. The nonadditive genetic effects were more strongly expressed when reared on chickpeas, a novel host,...

Leucine-rich fibroin gene of the Japanese wild silkmoth, Rhodinia fugax (Lepidoptera: Saturniidae)


Eur. J. Entomol. 105 (4): 561-566, 2008 | 10.14411/eje.2008.075

We cloned and characterized a partial fibroin gene of Rhodinia fugax (Saturniidae). The gene encodes a fibroin consisting mainly of orderly arranged repeats, each of which is divided into a polyalanine and a nonpolyalanine block, similar to the fibroins of Antheraea pernyi and A. yamamai. Three repeat types differ in the sequence of the nonpolyalanine block. In contrast to the Antheraea fibroins, the fibroin of R. fugax is rich in glutamate and leucine residues (about 3% and 5%, respectively) and contains less alanine.

Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution

Zhong Zheng GUI, Bo Yeon KIM, Kwang Sik LEE, Ya Dong WEI, Xijie GUO, Hung Dae SOHN, Byung Rae JIN

Eur. J. Entomol. 105 (4): 567-574, 2008 | 10.14411/eje.2008.076

Two glutathione S-transferase (GST) cDNAs, GSTD2 and GSTS2, were cloned from the silkworm Bombyx mori. The B. mori GSTD2 (BmGSTD2) gene spans 4371 bp and consists of four introns and five exons that encode 222 amino acid residues. The deduced amino acid sequence of BmGSTD2 showed 58% protein sequence identity to the Delta-class GST of Maduca sexta. The B. mori GSTS2 (BmGSTS2) gene spans 3470 bp and consists of three introns and four exons that encode 206 amino acid residues. The deduced amino acid sequence of BmGSTS2 revealed 67%, 63%, and 61% protein sequence identities to the Sigma-class GSTs...

Regulation of the development of flight muscles in long-winged adults of the flightless bug Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae)

Radomír SOCHA, Jan ©ULA

Eur. J. Entomol. 105 (4): 575-583, 2008 | 10.14411/eje.2008.077

We studied the development of the indirect flight muscles and reproductive organs in long-winged (macropterous) adults of the flightless bug Pyrrhocoris apterus (L.) and the factors involved in flight muscles histolysis by means of total protein analysis. Both the extirpation of the corpus allatum, an endocrine gland that is the sole source of juvenile hormone, and sham operation accelerated histolysis and decreased the level of the total protein content of the flight muscles to the same extent. Degeneration of flight muscles was not a result of allatectomy but rather a consequence of injury, followed by resumption of enhanced food intake, because...

Light-induced behavioural effects on the locomotor activity rhythm of the blow fly, Calliphora vicina (Diptera: Calliphoridae)


Eur. J. Entomol. 105 (4): 585-590, 2008 | 10.14411/eje.2008.078

Under continuous light (LL) of low intensity (up to 2.0 lux), the free-running period of the blow fly activity rhythm lengthened for flies whose initial τDD was less than about 25 h, but shortened for longer-period flies. Light intensity above about 2.0 lux led to an overt behavioural arrhythmicity. A comparison of phases of the oscillation after LL as a function of phases before LL, however, indicated that the oscillation continued its motion even though overt behaviour was arrhythmic. As the intensity during LL increased to 48 lux, phases after LL became centred on a narrow range close to CT 12. Extended periods of brighter light are...

Endogenous 20-hydroxyecdysone levels in the haemolymph of non-diapause-destined and diapause-destined generations of tasar silkworm, Antheraea mylitta (Lepidoptera: Saturniidae) and associated developmental changes

Dinesh KUMAR, Bhattiprolu SUBRAHMANYAM, Shashi K. SHARAN, Pradeep K. MISHRA, Bishwa M.K. SINGH, Nagabathula SURYANARAYANA

Eur. J. Entomol. 105 (4): 591-598, 2008 | 10.14411/eje.2008.079

A complete profile of the 20-hydroxyecdysone (20-HE) titer, development and endocrine events from 1st instar to pupation of the larvae of non-diapause-destined (NDD) and diapause-destined (DD) tasar silkworm, Antheraea mylitta Drury (Lepidoptera: Saturniidae) was studied. Diapause is induced by short days of 11 hr photophase coupled with <= 24°C prevailing in September-November. Diapausing pupae produce adults in July (>= 12h light, >= 26°C) and one generation is completed by August. The growth rate during the course of development of larval instars decreases and instar durations are inversely related to the body...

The effect of temperature on the diapause and cold hardiness of Dendrolimus tabulaeformis (Lepidoptera: Lasiocampidae)

Ju-Ping ZENG, Feng GE, Jian-Wei SU, Yong WANG

Eur. J. Entomol. 105 (4): 599-606, 2008 | 10.14411/eje.2008.080

Pine caterpillar, Dendrolimus tabulaeformis Tsai et Liu, is a major pine pest in North China. The larvae enter diapause in the third or fourth instar before winter. Supercooling points (SCP) and cold hardiness of the diapausing larvae were investigated and compared in non-acclimated, acclimated and de-acclimated larvae. A bimodal frequency distribution was observed with a break point of -14°C in the SCP. Larvae in the low group (LG, SCP <= -14°C) were more cold tolerant with lower lethal temperatures than those in the high group (HG, SCP > -14°C). This bimodality occurred in three patterns, LG (> 60% of individuals in LG), LG-HG...

Influence of pre-diapause temperature on intensity of summer and winter diapause in the cabbage butterfly Pieris melete (Lepidoptera: Pieridae)

Hai-Jun XIAO, Hai-Min HE, Feng LI, Fang-Sen XUE

Eur. J. Entomol. 105 (4): 607-611, 2008 | 10.14411/eje.2008.081

Effect of pre-diapause temperature on summer and winter diapause intensity was examined under both laboratory and field conditions. Under short photoperiods of 8L : 16D and 10L : 14D, all pupae entered diapause at 15, 18 and 20°C and the incidence of diapause dropped to 82.3% and 85.5% at 22°C, respectively. Under long photoperiods of 14L : 10D and 16L : 8D, the incidence of diapause decreased with increasing temperature and there were significant differences among temperatures. The incidence of diapause at 16L : 8D was significantly lower than that under14L : 10D at 20 and 22°C. By transferring diapause pupae induced under various temperatures (18,...

Role of volatile and contact pheromones in the mating behaviour of Bagrada hilaris (Heteroptera: Pentatomidae)

Salvatore GUARINO, Claudio DE PASQUALE, Ezio PERI, Giuseppe ALONZO, Stefano COLAZZA

Eur. J. Entomol. 105 (4): 613-617, 2008 | 10.14411/eje.2008.082

Volatiles and contact pheromones involved in the mating behaviour of the Painted bug, Bagrada hilaris Burmeister (Heteroptera: Pentatomidae), were investigated in behavioural and chemical experiments. Vertical open Y-shaped olfactometer bioassays showed that odour from males attract females but not males, while that from females did not attract either gender. Adult females were also attracted by hexane extracts of volatile compounds collected from males. In open arena bioassays, males displayed the characteristic steps of courtship behaviour in the presence of virgin females. Such courtship behaviour was displayed in the presence of females...

Role of excreta in predator avoidance by the Kanzawa spider mite, Tetranychus kanzawai (Acari: Tetranychidae)

Keiko OKU

Eur. J. Entomol. 105 (4): 619-623, 2008 | 10.14411/eje.2008.083

The Kanzawa spider mite, Tetranychus kanzawai (Acari: Tetranychidae) constructs webs over leaf surfaces and usually lives under these webs. T. kanzawai produces two types of excreta, black and yellow pellets, and uses its webs as a place for excretion. T. kanzawai also uses its webs as a refuge when the predatory mite, Neoseiulus womersleyi (Acari: Phytoseiidae) is present. To clarify what factors deter N. womersleyi from foraging on T. kanzawai webs, I experimentally examined the effects of T. kanzawai excreta on its own fitness (fecundity) and the foraging behaviour of N. womersleyi. When the...

Effect of larval crowding on the life history traits of Sesamia nonagrioides (Lepidoptera: Noctuidae)


Eur. J. Entomol. 105 (4): 625-630, 2008 | 10.14411/eje.2008.084

This study determined the effect of larval density-dependent competition for food on development and adult fitness in Sesamia nonagriodes Lef. (Lepidoptera: Noctuidae). Different numbers (5, 10, 15, 20 and 30 individuals) of larvae of the pink stalk borer were reared on a constant amount of food. Although crowding during the immature stages did not significantly increase mortality, it prolonged the larval developmental period and resulted in reduced pupal weight. Females were more adversely affected by high density than males, resulting in lighter females, indicating that female growth is more sensitive to density. The fecundity of the adults...

Substrate particle size-preference of wormlion Vermileo vermileo (Diptera: Vermileonidae) larvae and their interaction with antlions


Eur. J. Entomol. 105 (4): 631-635, 2008 | 10.14411/eje.2008.085

Wormlion larvae are found in substrates consisting of fine sand or powder, implying that they may be able to distinguish between different substrates according to particle size. To estimate the effects of particle size on wormlions, the pit-building decision of the larvae of the wormlion Vermileo vermileo was observed in four substrates consisting of different sand fractions. Wormlion larvae prefer the finest sand fraction with particle size <= 230 µm. When wormlions (Vermileo vermileo) and antlions (Euroleon nostras) are placed in the same container with two different substrates, interspecific predation...

Myrmica sabuleti (Hymenoptera: Formicidae) not necessary for the survival of the population of Phengaris (Maculinea) arion (Lepidoptera: Lycaenidae) in eastern Poland: Lower host-ant specificity or evidence for geographical variation of an endangered social parasite?


Eur. J. Entomol. 105 (4): 637-641, 2008 | 10.14411/eje.2008.086

Larvae and pupae of the obligatorily myrmecophilous Phengaris (Maculinea) arion were found for the first time in nests of Myrmica rugulosa, M. hellenica and M. schencki at a site in eastern Poland. M. sabuleti, commonly considered as a major host of the butterfly and whose abundance is vital for the survival of P. arion populations, was shown to be very rare and not used. Estimated mean density of Myrmica nests was very low, which suggests that relatively vast areas are necessary to support a viable population of P. arion in similar sandy habitats. This may explain...

Miller J.C., Janzen D.H. & Hallwachs W.: 100 Butterflies and moths: PoRtraits from the tropical forests of Costa Rica.


Eur. J. Entomol. 105 (4): 642, 2008 | 10.14411/eje.2008.087

The Belknap Press of Harvard University Press, Cambridge, 2007, 256 pp., ISBN 978-0-674-02334-5.

Experimental evidence for competitive exclusion of Myzus persicae nicotianae by Myzus persicae s.s. (Hemiptera: Aphididae) on sweet pepper, Capsicum annuum (Solanaceae)


Eur. J. Entomol. 105 (4): 643-648, 2008 | 10.14411/eje.2008.088

The importance of interspecific competition as a force promoting specialization in phytophagous insects has been long debated. Myzus persicae sensu stricto (Hemiptera: Aphididae) is one of the most polyphagous aphids. Its subspecies, M. persicae nicotianae, is found mainly on tobacco, although it can survive and reproduce on a relatively wide range of plant species in the laboratory. Since life history traits of these taxa make competitive interactions likely, we hypothesize that asymmetrical competition occurs between M. p. nicotianae and M. persicae s.s., and accounts for the exclusion of the former when they share a common...

Resources for British butterflies (Lepidoptera: Hesperioidea, Papilionoidea). The alien consumer component and its significance for butterfly habitats

Peter B. HARDY, Roger L.H. DENNIS

Eur. J. Entomol. 105 (4): 649-657, 2008 | 10.14411/eje.2008.089

With climatic warming there is an expectation that phytophagous insects will increasingly use alien (non native) plants as nectar sources and larval host plants. Alien plant use is investigated in British butterflies. Butterflies are considered to be larval host plant specialists relative to their use of nectar plants. Supporting this view, use of alien plants as nectar sources (50.1%, 27 novel plant families) is almost twice that of their use as larval host plants (21.6%; three novel plant families). Some 80% of the variation in percent alien nectar plant use is accounted against 30% of that for percent alien host plant use. The key variable accounting...

The classification of insect communities: Lessons from orthopteran assemblages of semi-dry calcareous grasslands in central Germany


Eur. J. Entomol. 105 (4): 659-671, 2008 | 10.14411/eje.2008.090

Whereas the classification of plant communities has a long tradition that of animal assemblages remains poorly developed. Here we propose a classification scheme for orthopteran communities based on regional "character species", "differential species" and "attendant species" at different levels of habitat complexity, which is also applicable to other insect groups. In this context there are three main points of special importance: (i) the geographical reference area, (ii) the hierarchical spatial level (e.g. habitat complex, habitat and microhabitat) and (iii) precise constancy criteria for the definition of character species and differential species....

Orthopteran communities in the conifer-broadleaved woodland zone of the Russian Far East


Eur. J. Entomol. 105 (4): 673-680, 2008 | 10.14411/eje.2008.091

We investigate orthopteran communities in the natural landscape of the Russian Far East and compare the habitat requirements of the species with those of the same or closely related species found in the largely agricultural landscape of central Europe. The study area is the 1,200 km2 Lazovsky State Nature Reserve (Primorsky region, southern Russian Far East) 200 km east of Vladivostok in the southern spurs of the Sikhote-Alin Mountains (134°E/43°N). The abundance of Orthoptera was recorded in August and September 2001 based on the number present in 20 randomly placed 1 m2 quadrates per site. For each plot (i) the number of species...

Establishment of ant communities in forests growing on former agricultural fields: Colonisation and 25 years of management are not enough (Hymenoptera: Formicidae)


Eur. J. Entomol. 105 (4): 681-689, 2008 | 10.14411/eje.2008.092

Ants are often considered as good indicators of change of management in different habitats. They have been used sporadically to evaluate large scale transformations involving reafforestation. In the present study, the ant assemblages at 15 forest sites are compared. The sites differ in history, age and forest management. Our results show that there are clear changes in ant assemblages with developmental stage but even after 25 years not all forest species are present. Natural succession and planting of trees with or without any other management, resulted in only open country species, and some from forest and wet grassland colonizing these new forests....

Löbl I. & Smetana A. (eds): CATALOGUE OF PALAEARCTIC COLEOPTERA. Vol. 3, Scarabaeoidea, Scirtoidea, Dascilloidea, Buprestoidea, Byrrhoidea, Vol. 4, Elateroidea, Derodontoidea, Bostrichoidea, Lymexyloidea, Cleroidea, Cucujoidea, Vol. 5, Tenebrionoidea.


Eur. J. Entomol. 105 (4): 690, 2008 | 10.14411/eje.2008.093

Löbl I. & Smetana A. (eds): CATALOGUE OF PALAEARCTIC COLEOPTERA. Vol. 3, Scarabaeoidea, Scirtoidea, Dascilloidea, Buprestoidea, Byrrhoidea, 2006, 690 pp., ISBN 87-88757-59-5; Vol. 4, Elateroidea, Derodontoidea, Bostrichoidea, Lymexyloidea, Cleroidea, Cucujoidea, 2007, 935 pp., ISBN 87-88757-67-6; Vol. 5, Tenebrionoidea, 2008, 670 pp., ISBN 978-87-88757-69-9; Apollo Books, Stenstrup. ISBN 87-88757-71-4 or 978-87-88757-71-2 (Vols 1-8) (hardbacks).

Insects on decomposing carcasses of small rodents in a secondary forest in Southeastern Brazil

Thiago De Carvalho MORETTI, Odair Benedito RIBEIRO, Patrícia Jacqueline THYSSEN, Daniel Russ SOLIS

Eur. J. Entomol. 105 (4): 691-696, 2008 | 10.14411/eje.2008.094

The decomposition of small carcasses in the open is frequently neglected although it may provide information of forensic importance. This paper describes an experimental study of arthropod species associated with carcasses of mouse, Mus musculus (Linnaeus, 1758) and rat, Rattus norvegicus (Berkenhout, 1769) (Rodentia: Muridae). Four carcasses were left inside iron cages in sunlit and shady areas in a secondary forest in Southeastern Brazil twice a season for four seasons (n = 16 carcasses of each rodent). The carcasses were removed when arthropods ceased to visit them. The visiting and colonizing invertebrates were collected daily and...

The developmental process during metamorphosis that results in wing reduction in females of three species of wingless-legged bagworm moths, Taleporia trichopterella, Bacotia sakabei and Proutia sp. (Lepidoptera: Psychidae)


Eur. J. Entomol. 105 (4): 697-706, 2008 | 10.14411/eje.2008.095

There are several evolutionary grades of wing reduction in female bagworm moths of the family Psychidae. In this family, female adults of Taleporia trichopterella, Bacotia sakabei and Proutia sp. have vestigial wings, although as pupae they have small wings. Consequently, these species (usually called wingless-legged bagworm moths), are intermediate between the two extremes of females with normal wings and those with no wings. Using light and electron microscopy, the processes of wing development during the last-larval instar and wing degeneration during the pupal stage was investigated in these species. Female wing imaginal discs...

Morphology and ultrastructure of the male reproductive system of the woolly beech aphid Phyllaphis fagi (Hemiptera: Aphididae: Phyllaphidinae)


Eur. J. Entomol. 105 (4): 707-712, 2008 | 10.14411/eje.2008.096

In the present paper we describe for the first time the ultrastructure of the male reproductive system of aphids using Phyllaphis fagi as a representative. Paired testes of this species consist of three follicles each, arranged in a rosette, the walls of the proximal part of vasa deferentia cling together, accessory glands are club-shaped and elongated whereas the ejaculatory duct is reduced. Our study also shows that vasa deferentia, accessory glands and ejaculatory duct are histologically very simple. All of them are composed of cubical epithelium of secretory type. The epithelial cells are rich in rough endoplasmic reticulum, Golgi complexes...

Rapid discrimination of the common species of the stored product pest Liposcelis (Psocoptera: Liposcelididae) from China and the Czech Republic, based on PCR-RFLP analysis

Meng QIN, Zhi-Hong LI, Zuzana KUÈEROVÁ, Yang CAO, Václav STEJSKAL

Eur. J. Entomol. 105 (4): 713-717, 2008 | 10.14411/eje.2008.097

Psocids of the genus Liposcelis (Psocoptera: Liposcelididae) are stored product pests that are difficult to identify morphologically. A molecular method based on Restriction Fragment Length Polymorphism (RFLP) of the PCR-amplified 16S rDNA gene was developed for the rapid discrimination of four common species (L. bostrychophila, L. entomophila, L. decolor, and L. paeta). Different developmental stages and populations (P.R. China and Czech Republic) were tested. One DNA fragment of about 500 bp in length was amplified from genomic DNA and the fragment was then digested using the restriction endonuclease...

Domínguez E., Molineri C., Pescador M.L., Hubbard M.D. & Nieto C.: EPHEMEROPTERA OF SOUTH AMERICA


Eur. J. Entomol. 105 (4): 718, 2008 | 10.14411/eje.2008.098

Pensoft Publishers, Sofia-Moscow, 2006, vi + 640 pp. ISSN 13127276/ ISBN-10: 954-642-259-2, ISBN-13: 978-954-642-259-0. Price EUR 115.00.

Taxonomical revision of the Longitarsus capensis species-group: An example of Mediterranean-southern African disjunct distributions (Coleoptera: Chrysomelidae)


Eur. J. Entomol. 105 (4): 719-736, 2008 | 10.14411/eje.2008.099

Southern-African Longitarsus capensis species-group, which is closely related to the Mediterranean anchusae species-group is revised. L. capensis species-group includes 15 species, 8 of which are new to science: Longitarsus capensis Baly, 1877, L. cedarbergensis Biondi, 1999, L. luctuosus Biondi, 1999, L. lugubris Biondi, 1999, L. melanicus Biondi, 1999, L. neseri Biondi, 1999, L. transvaalensis Biondi, 1999, L. afromeridionalis sp.n., L. debiasei sp.n., L. grobbelaariae sp.n., L. hexrivierbergensis sp.n., L. malherbei sp.n., L. piketbergensis...

On the systematic position of the diving-beetle genus Pachydrus (Coleoptera: Dytiscidae: Hydroporinae): Evidence from larval chaetotaxy and morphology

Mariano C. MICHAT, Patricia L.M. TORRES

Eur. J. Entomol. 105 (4): 737-750, 2008 | 10.14411/eje.2008.100

Phylogenetic relationships within the diving-beetle subfamily Hydroporinae are not well understood. Some authors include the genus Pachydrus Sharp, 1882 in the tribe Hyphydrini, whereas others are in favour of excluding Pachydrus from the Hyphydrini and placing it in its own tribe, Pachydrini. Larval characters have been underutilised in phylogenetic studies, mainly because the larvae of many taxa within the family are unknown. In this study, the phylogenetic relationships of Pachydrus are studied based on a cladistic analysis of 34 taxa and 122 morphological larval characters. For this purpose, larvae of P. obesus Sharp,...

Revision of Xyalophora Kieffer and description of Xyalophoroides gen. n. (Hymenoptera: Figitidae: Figitinae)


Eur. J. Entomol. 105 (4): 751-769, 2008 | 10.14411/eje.2008.101

The genus Xyalophora (Giraud, 1860) is revised herein. The revision includes the type species of Xyalophora (Figites clavatus Giraud, 1860), the type material and the original descriptions of all the species of Xyalophora included in the Weld catalogue, and long series of undetermined material. Xyalophora clavata (Giraud, 1860) and X. singularis (Ashmead, 1896) are the only currently recognized species that should be included in Xyalophora. Four new species are described: X. belizini sp. n., X. giraudi sp. n., X. provancheri sp. n. and X. zarazagai sp. n. The type species...

Revision of Phaeochiton Kerzhner, 1964 (Heteroptera: Miridae: Phylini)


Eur. J. Entomol. 105 (4): 771-781, 2008 | 10.14411/eje.2008.102

The genus Phaeochiton Kerzhner, 1964 is revised and P. alenae sp. n. from Mongolia is described. Differential diagnoses are provided for the genus and its three species. Illustrations of male and female genitalia, tarsus and pretarsus, photographs of the dorsal habitus, hosts, and distributional records of the species discussed are given.

A new genus of isophlebioid damsel-dragonflies with "calopterygid"-like wing shape from the Middle Jurassic of China (Odonata: Isophlebioidea: Campterophlebiidae)

André NEL, Di-Ying HUANG, Qi-Bin LIN

Eur. J. Entomol. 105 (4): 783-787, 2008 | 10.14411/eje.2008.103

reni, a new campterophlebiid genus and species is described from the Middle Jurassic of China. This fossil has a wing shape unique for this clade, i.e. a fore- and hind wing of the same width and very shortly petiolated, and hind wing cubito-anal area nearly as narrow as that of the forewing. This wing shape is convergently similar to that of recent Zygoptera: Calopterygidae, as well as to several other Cenozoic zygopteran clades, suggesting similar styles of flight and habits, i.e. predation on small insects and flight along trees of river banks.

Polygraphus grandiclava (Coleoptera: Curculionidae) collected from pine and cherry trees: A phylogenetic analysis

Dimitrios AVTZIS, Milo¹ KNÍ®EK, Klaus HELLRIGL, Christian STAUFFER

Eur. J. Entomol. 105 (4): 789-792, 2008 | 10.14411/eje.2008.104

Polygraphus grandiclava
(Thomson, 1886) is a unique scolytid species in that it infests both Pinaceae and Rosaceae. The utilization of such different host trees lead to the designation of two species at the beginning of the last century. Later on, these two species were synonymised. Here we investigated the genetic identity of populations collected from pine and cherry trees by sequencing a partial region of the mitochondrial COI gene. The phylogenetic study presented reveals no indication of host-induced differentiation within the mitochondrial sequences of the populations collected from the two host plants.

A modified method for clearing, staining and mounting plant-inhabiting mites


Eur. J. Entomol. 105 (4): 793-795, 2008 | 10.14411/eje.2008.105

A modified method for clearing, staining and mounting plant-inhabiting mites is proposed. With this method, all mites can be cleared with a single agent and mounted in a single medium. The stain added to the mounting medium enhances the clarity of the fine structures of soft-bodied mites under phase contrast and more significantly under bright field microscopy.