EJE, vol. 101 (2004), issue 2

Environmental regulation of the purine synthesis enzyme purH transcript during adult diapause in Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

George D. YOCUM

Eur. J. Entomol. 101 (2): 199-203, 2004 | DOI: 10.14411/eje.2004.025

A 1962 bp cDNA clone of 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (purH) was isolated from diapausing adults of Leptinotarsa decemlineata using RT-PCR and 3' and 5'-RACE. The probe generated from this clone hybridized to a transcript approximately 1960 bp in length on northern blots. The clone encodes for a deduced protein 594 amino acids in length with 73% identity, 83% similarity to purH from Drosophila melanogaster. Northern blot (total RNA) analysis determined that L. decemlineata purH (LdpurH) was downregulated in diapausing beetles stored at 10C. Developmental...

New cytogenetic data on Nabidae (Heteroptera: Cimicomorpha), with a discussion of karyotype variation and meiotic patterns, and their taxonomic significance


Eur. J. Entomol. 101 (2): 205-210, 2004 | DOI: 10.14411/eje.2004.026

As a part of ongoing cytogenetic studies on the bug family Nabidae (Heteroptera), the karyotypes and meiotic patterns of male Nabis (Aspilaspis) viridulus Spinola, 1837, N. (A.) indicus (Stål, 1873) (subfamily Nabinae) and Prostemma guttula (Fabricius, 1787) (subfamily Prostemmatinae) are described.
N. viridulus and N. indicus differ from P. guttula in their chromosome numbers, which are 2n = 32 + XY and 2n = 26 + XY, respectively, and behaviour of the sex chromosomes in male meiosis, which, respectively, show "distance pairing" and "touch-and-go pairing" in spermatocyte metaphase II. The karyotype...

Cytogenetic studies on Mepraia gajardoi (Heteroptera: Reduviidae). Chromosome behaviour in a spontaneous translocation mutant

Ruben PREZ, Lucia CALLEROS, Virginia ROSE, Myriam LORCA, Francisco PANZERA

Eur. J. Entomol. 101 (2): 211-218, 2004 | DOI: 10.14411/eje.2004.027

We analysed the chromosome complement and male meiosis in a natural population of Mepraia gajardoi (Hemiptera: Reduviidae: Triatominae). The normal karyotype of this species is 20 autosomes plus X1X2Y in males and X1X1X2X2 in females. We confirmed that M. gajardoi is cytogenetically strikingly different from M. spinolai (with which it was previously included), which supports the taxonomic separation of these two species. For the first time in the holocentric chromosomes of the subfamily Triatominae, a structural rearrangement was detected. Observations...

Patterns in abdominal pumping, miniature inspirations and heartbeats simultaneously recorded during cyclical gas exchange in adult Hylobius abietis (Coleoptera: Curculionidae) using a respirometer and IR actographs


Eur. J. Entomol. 101 (2): 219-225, 2004 | DOI: 10.14411/eje.2004.028

Rhythms in abdominal pumping, heartbeats and discontinuous gas exchange cycles (DGCs) of CFV subtype (closed, flutter and ventilation periods) in adults of pine weevil Hylobius abietis were simultaneously recorded using an electrolytic microrespirometer and an infrared (IR) cardiograph or IR actograph (IRA). The cyclic release of carbon dioxide was associated with active ventilation (V phase) provided by vigorous abdominal pumping movements due to contractions of tonic longitudinal and transversal abdominal muscles, which are visible to the naked eye. On the IRA-recordings, the abdominal pumping signals are superimposed on the heartbeat spikes....

BOOK REVIEW: Anderson D.T.: Invertebrate Zoology.


Eur. J. Entomol. 101 (2): 226, 2004 | DOI: 10.14411/eje.2004.029

Oxford University Press, Melbourne. 1st edition: 1998, 467 pp. ISBN 0-19-553941-9 (paperback). 2nd edition: 2001, 512 pp. ISBN 0195513681 (paperback). Price AU$ 80.00.

Ability of primary and secondary reproductives to inhibit the development of neotenics in Kalotermes flavicollis (Isoptera: Kalotermitidae)


Eur. J. Entomol. 101 (2): 227-230, 2004 | DOI: 10.14411/eje.2004.030

The inhibitory potential of primary and secondary reproductives was studied using half-orphaned colonies of Kalotermes flavicollis. Both primary and secondary reproductives (neotenics) were equally effective in inhibiting the development of replacement reproductives. Single females totally inhibited the development of female secondary reproductives but did not affect the development of male secondary reproductives. Single males had neither a stimulatory nor inhibitory effect on the development of secondary reproductives. The inhibitory ability of pairs of primary reproductives shortly after dealation and at the stage of incipient colony formation...

Supercooling capacity and cold hardiness of the eggs of the grasshopper Chorthippus fallax (Orthoptera: Acrididae)

Shu-Guang HAO, Le KANG

Eur. J. Entomol. 101 (2): 231-236, 2004 | DOI: 10.14411/eje.2004.031

Cold tolerance of the eggs of the grasshopper, Chorthippus fallax (Zubovsky), was examined in the laboratory. Egg supercooling points varied from -6C to -32.4C and could be divided into two groups. The supercooling points of the higher SCP group ranged from -6C to -14C and those of lower SCP group from -21.8C to -32.4C. Although low temperature acclimation could slightly decrease the supercooling points of eggs, the effect was not significant for all embryonic developmental stages or acclimation periods. The supercooling capacity was obviously different between pre-diapause, diapause and post-diapause embryonic stages. The mean...

Intraguild predation between the aphidophagous ladybird beetles Harmonia axyridis and Coccinella undecimpunctata (Coleoptera: Coccinellidae): the role of body weight

Sandra FLIX, Antnio Onofre SOARES

Eur. J. Entomol. 101 (2): 237-242, 2004 | DOI: 10.14411/eje.2004.032

The magnitude, direction and symmetry of intraguild predation (IGP) between the developmental stages of the aphidophagous Harmonia axyridis Pallas and Coccinella undecimpunctata L. were characterized and compared. Mobility and body weight were the main factors affecting the magnitude of IGP.
H. axyridis was more often the predator than C. undecimpunctata and eggs the most vulnerable developmental stage. There was significant asymmetrical IGP on eggs by the second and later, and fourth larval stages of H. axyridis and C. undecimpunctata, respectively. Asymmetrical IGP of pupae was only recorded...

Effects of parasitoid associated factors of the endoparasitoid Glyptapanteles liparidis (Hymenoptera: Braconidae)


Eur. J. Entomol. 101 (2): 243-249, 2004 | DOI: 10.14411/eje.2004.033

The endoparasitoid Glyptapanteles liparidis (Hymenoptera: Braconidae) injects polydnavirus and venom together with eggs into its Lymantria dispar (Lepidoptera: Lymantriidae) host larvae during oviposition. We studied effects of these parasitoid associated factors by means of g-irradiation-induced pseudoparasitization. The application of radiation for sterilization of female wasps as a tool to study interactions between parasitoid associated factors and the host is demonstrated in this paper. When wasps were irradiated at doses ranging from 24 to 96 Gy and then allowed to oviposit into L. dispar larvae, temporary sterilization...

BOOK REVIEW: Simon J.C., Dedryver C.A., Rispe C. & Hulle M. (eds): Aphids in a New Millenium.


Eur. J. Entomol. 101 (2): 250, 2004 | DOI: 10.14411/eje.2004.034

Proceedings of the 6th International Symposium on Aphids, September 2001, Rennes, France. INRA Editions, Versailles-Paris, 2004, 549 pp. ISBN 2-7380-113-6. Price EUR 62.00.

Host specificity or habitat structure? - The epicortical beetle assemblages in an Australian subtropical rainforest


Eur. J. Entomol. 101 (2): 251-259, 2004 | DOI: 10.14411/eje.2004.035

We have investigated the relative importance of bark roughness and host tree species in determining the diversity and make-up of coleopteran assemblages on the trunks of trees in sub-tropical rainforest in south-east Queensland, Australia. There are clear, statistically significant, patterns in the composition of the assemblages which reflect bark roughness. Rougher bark had associated greater species and family richness, a higher proportion of "rare" species, a higher value for Shannon diversity and lower values for the Simpson and Berger-Parker Index (measures of dominance within the sample). The data support strongly the idea that there is a bark-specific...

Immature stages of New Caledonian Tingidae (Heteroptera): Description and development


Eur. J. Entomol. 101 (2): 261-271, 2004 | DOI: 10.14411/eje.2004.036

Second to fifth instars of nine New Caledonian species of Tingidae are described. A key to fifth instars is provided. The comparison of immature stages among the known larvae shows that translocation of marginal tubercles in larvae, considered as the general rule, is absent in New Caledonian larvae. Also, external morphology of larvae becomes more complex through development, by the addition and ramification of tubercles, as it does also through evolution. Such a tendency is reminiscent of a heterochronous phenomenon, like peramorphosis. Translocation phenomenon could be an intermediate stage of evolution in terms of aquiring more tubercles.

BOOK REVIEW: Nickel H.: The Leafhoppers and Planthoppers of Germany (Hemiptera, Auchenorrhyncha). Patterns and Strategies in a Highly Diverse Group of Phytophagous Insects.


Eur. J. Entomol. 101 (2): 272, 2004 | DOI: 10.14411/eje.2004.037

Pensoft Series Faunistica No. 28. Pensoft Publishers, Sofia-Moscow wt Goecke & Evers, Keltern, 2003, 460 pp. ISSN 1312-0174. Hardcover. Price USD 67.00

Review of larval morphology of beetles of the suborder Archostemata (Insecta: Coleoptera), including first-instar chaetotaxy


Eur. J. Entomol. 101 (2): 273-292, 2004 | DOI: 10.14411/eje.2004.038

This paper presents a synthesis of morphological information on larvae of the beetle suborder Archostemata. Larvae of the following families and species were studied: Ommatidae: Omma sp.; Micromalthidae: Micromalthus debilis LeConte, 1878; Cupedidae: Priacma serrata LeConte, 1861, Distocupes varians (Lea, 1902), Rhipsideigma raffrayi (Fairmaire, 1884), Tenomerga cinerea (Say, 1831) and Tenomerga mucida (Chevrolat, 1829). Morphological characters of the suborder and three families are described. Monophyly of the suborder is strongly supported by more than 10 larval...

Larval morphology of three species of Hygrobiidae (Coleoptera: Adephaga: Dytiscoidea) with phylogenetic considerations


Eur. J. Entomol. 101 (2): 293-311, 2004 | DOI: 10.14411/eje.2004.039

A provisional larval groundplan of the family Hygrobiidae is provided through descriptions of internal and external features of three of six extant species, Hygrobia hermanni (Fabricius, 1775), H. wattsi Hendrich 2001 and H. australasiae (Clark, 1862) and phylogenetic interpretations. Hygrobiidae larvae are morphologically differing dramatically from all other known Adephaga by 20 autapomorphies. Structures involved with feeding, i.e., mouthparts, prepharynx and foregut are highly modified as a result of a specialisation on small tubificid worms and chironomid larvae. A placement of Hygrobiidae within Dytiscoidea is well supported...

Description of the first instar larvae of three species of Meloe with a key to the triungulins of Central European species of this genus (Coleoptera: Meloidae)

Johannes LCKMANN, Siegmund SCHARF

Eur. J. Entomol. 101 (2): 313-322, 2004 | DOI: 10.14411/eje.2004.040

The first instar larvae (triungulins) of Meloe (Meloegonius Reitter) rufiventris Germar, 1817, M. (Micromeloe Reitter) uralensis Pallas, 1777 and M. (Eurymeloe Reitter) scabriusculus Brandt & Erichson, 1832 are described, which were before unknown. The systematic relationship to closely related species is discussed. A key is included which allows to identify all triungulins of the genus Meloe from Central Europe.

Number and position of wounds on honey bee (Apis mellifera) pupae infested with a single Varroa mite


Eur. J. Entomol. 101 (2): 323-326, 2004 | DOI: 10.14411/eje.2004.041

The wounds inflicted on pupae in capped brood cells of the honey bee, Apis mellifera, infested with a single female of the ectoparasitic mite, Varroa destructor, were investigated after visualisation by vital staining with trypan blue. On average the mites made two integumental perforations for feeding on prepupae and one on pupae. Most of the punctures were on particular ventral sites on the abdomen. Possible reasons for this pronounced preference and the evolutionary aspects of this highly specialised parasite-host relationship are discussed.

The effect of heat stress on the survival of the rose grain aphid, Metopolophium dirhodum (Hemiptera: Aphididae)

Chun-Sen MA, Bernhard HAU, Hans-Michael POEHLING

Eur. J. Entomol. 101 (2): 327-331, 2004 | DOI: 10.14411/eje.2004.042

The rose grain aphid, Metopolophium dirhodum (Walker) (Hemiptera: Aphididae) is one of the three most important cereal aphid species in Europe. High temperature is detrimental for the survival of this species. Detailed experiments were conducted on the effect of high temperature (27, 28, 29, 30, 31, 31.5, 32.5, 33 and 34C), period of exposure (2, 3, 4, 6 and 8 h per day for 1, 2, 4, 6 days) and developmental stage (2nd, 3rd, 4th instar nymph and adult) on the survival of the aphid. The results show that all three factors significantly affect survival. Temperatures over 29C for 8 h significantly reduced survival, which decreased generally as...

BOOK REVIEW: Jongjean F. & Kaufman W.R. (Eds): Ticks and Tick-Borne Pathogens.


Eur. J. Entomol. 101 (2): 332, 2004 | DOI: 10.14411/eje.2004.043

Kluwer Academic Publishers, Dordrecht, The Netherlands, 2003, 309 pp.

Instar preference and parasitization of Aphis gossypii and Myzus persicae (Hemiptera: Aphididae) by the parasitoid Aphidius colemani (Hymenoptera: Aphidiidae)

Dionyssios C. PERDIKIS, Dionyssios P. LYKOURESSIS, Nikolaos G. GARANTONAKIS, Stylianh A. IATROU

Eur. J. Entomol. 101 (2): 333-336, 2004 | DOI: 10.14411/eje.2004.044

The instar preference and parasitization (expressed as mummification rate) of Aphis gossypii Glover and Myzus persicae (Sulzer) (Hemiptera: Aphididae) by the parasitoid Aphidius colemani Viereck (Hymenoptera: Aphidiidae) were studied at 25±0.5C, 65±5% r.h. and a 16L : 8D photoperiod. The female parasitoids were 24-36 h old and were left to forage for 1 h on an eggplant leaf on which 10 nymphs of each instar of A. gossypii or M. persicae were placed. The percentage of A. gossypii nymphs mummified was higher than that of M. persicae (43.2 and 25.2%, respectively). The parasitoid parasitized...

Some observations on the reproductive biology of the scuttle fly Megaselia andrenae (Diptera: Phoridae) at the nesting site of its host Andrena agilissima (Hymenoptera: Andrenidae)


Eur. J. Entomol. 101 (2): 337-340, 2004 | DOI: 10.14411/eje.2004.045

Some behavioural aspects of the reproductive biology of Megaselia andrenae Disney, a kleptoparasite of the communal bee Andrena agilissima (Scopoli), were investigated at the nesting site of its host at Isola d'Elba (Italy). The scuttle fly mates more often in the early afternoon, which coincides with the period when the provisioning flights of its host are more frequent. The presence of the flies at the host nesting site, either in copula or single, is lower in the morning. In general only the females enter the host nests immediately after a mate, in a few cases closely followed by the males. When leaving the nest, females refuse to...

Sex ratio of apollo butterfly Parnassius apollo (Lepidoptera: Papilionidae) - facts and artifacts


Eur. J. Entomol. 101 (2): 341-344, 2004 | DOI: 10.14411/eje.2004.046

The adult sex ratio among the genus Parnassius is usually strongly male biased and close to 2 : 1. This paper presents the results of comparative studies, on the basis of data from wild and captive reared populations of apollo butterfly in the Pieniny National Park (Western Carpathians, Poland). Sex ratio among the wild population is strongly male biased and close to 2 : 1, whereas there was no sex ratio bias among the captive population. However, among the captive individuals caught after releasing into the wild, males significantly outnumbered females. There was a significant, sex-related, difference in activity pattern recorded in the field...

OBITUARY: In memory of Dr. John D. Bradley FRES (24 December 1920 - 4 January 2004)


Eur. J. Entomol. 101 (2): 345, 2004 | DOI: 10.14411/eje.2004.047

At the beginning of this year, we read with great regret an e-mail from David Bradley on the death of his father Dr. John David Bradley. John was not only outstanding lepidopterist, but also a faithful friend of both of us and an extremely helpful English language editor of this journal. His contribution was invaluable, especially in the early years of Acta Entomologica Bohemoslovaca (predecessor of EJE) when the editors were striving to transform a local periodical into an internationally respected journal.
As a scientist at the Department Entomology of the British Museum (Natural History) and at the Identification Service of the...

BOOK REVIEW: Wheater C.P. and Cook P.A. 2003: Studying Invertebrates.


Eur. J. Entomol. 101 (2): 346, 2004 | DOI: 10.14411/eje.2004.048

Naturalists' Handbooks 28. The Richmond Publishing, Slough, 120 pp.