EJE, vol. 115 (2018)

Molecular cloning and functional analyses of an adhesion molecule, neuroglian, in Mythimna separata (Lepidoptera: Noctuidae)

Kakeru YOKOI, Yoshiaki KATO, Masahiro SUZUKI, Ken MIURA

Eur. J. Entomol. 115: 157-166, 2018 | DOI: 10.14411/eje.2018.015

Insect cellular immune reaction, which generally includes phagocytosis, encapsulation and nodule formation, is achieved by hemocytes circulating in insect haemolymph. The shift of hemocytes from the normal phase to the adhering phase is an important process in the cellular immune reaction, which includes the attachment of hemocytes to foreign surfaces or other hemocytes via adhesion factors. Neuroglian is one of the adhering factors associated with encapsulation in Manduca sexta and Drosophila melanogaster. Here we studied the localization of neuroglian (MsNrg) in Mythimna separata and its functional role in the cellular immune...

Efficiency of two methods of sampling used to assess the abundance and species diversity of adult Syrphidae (Diptera) in mountainous meadows in the Austrian and Swiss Alps


Eur. J. Entomol. 115: 150-156, 2018 | DOI: 10.14411/eje.2018.014

The outcome of assessments of the biodiversity of a taxonomic group often depend on the sampling method. The choice of an adequate method is especially important for biomonitoring purposes. In this study, the effectiveness of two methods of sampling syrphids (Diptera: Syrphidae) is compared: observation plot method vs. line transect, both sampled by sweep netting. Altogether, 18 meadows were selected in three mountain regions in the Austrian and Swiss Alps. We recorded a significantly higher abundance and richness of syrphids using the observation plot method than the line transect method in 2015. Comparing data for one region recorded in 2015 and...

Influence of urbanisation and plants on the diversity and abundance of aphids and their ladybird and hoverfly predators in domestic gardens

Elise A. ROCHA, Estevão N.F. SOUZA, Lewis A.D. BLEAKLEY, Christopher BURLEY, Jade L. MOTT, Gloria RUE-GLUTTING, Mark D.E. FELLOWES

Eur. J. Entomol. 115: 140-149, 2018 | DOI: 10.14411/eje.2018.013

Urban gardens can harbour a high diversity of insects, which are critically important components of urban ecosystems. In this work, we investigate the richness and diversity of a major taxon of economic and ecological importance, the aphids (Hemiptera: Aphididae), and their main insect predators, the hoverflies (Diptera: Syrphidae) and ladybirds (Coleoptera: Coccinellidae), in urban gardens. We examined how variation in environmental factors associated with urbanisation (garden host plant abundance, garden plant richness, garden size, proportion of impervious surfaces in the surrounding area) directly and indirectly (via prey and predator abundance)...

Structure and function of the male ventral organ in Onychiuroides granulosus (Collembola: Onychiuridae)


Eur. J. Entomol. 115: 134-139, 2018 | DOI: 10.14411/eje.2018.012

On the ventral tube of males of Onychiuroides granulosus (Stach, 1934) there is a male ventral organ, which consists of two groups of four setae that are thickened, slightly flattened and bent. All setae of the male ventral organ are inserted in a richly sculptured cuticle. At the base of each seta there are a few large cells (basal cells) that have large irregular nuclei that contain a large amount of heterochromatin. In the cytoplasm of the basal cells there are numerous mitochondria, ribosomes and a rich system of endoplasmic reticulum. The plasma membrane of the basal cells forms richly folded, deep invaginations, filled with a dense material,...

New fossil genus and species of Sinoalidae (Hemiptera: Cercopoidea) from the Middle to Upper Jurassic deposits in northeastern China

Yan-Zhe FU, Di-Ying HUANG

Eur. J. Entomol. 115: 127-133, 2018 | DOI: 10.14411/eje.2018.011

A new fossil genus and species of Sinoalidae, Stictocercopis wuhuaensis gen. et sp. n., from the Middle to Upper Jurassic Haifanggou Formation at Daohugou, Inner Mongolia, northeastern China is described, illustrated and its systematic position discussed, on the basis of four complete well-preserved specimens. The new genus distinctly differs from other sinoalids in having relatively complex wing venation and tegmen spots. The intra-specific variation in venation is also discussed. The new discovery increases the palaeodiversity of sinoalids in the early assemblage of the Yanliao biota from the Daohugou beds.

Conspectus of Australian Brachystomellidae (Collembola) with description of new species of Rapoportella and redescription of Cassagnella anomala


Eur. J. Entomol. 115: 117-126, 2018 | DOI: 10.14411/eje.2018.010

As part of a modern review of all Australian Collembola families, a key is provided to the nine genera of Brachystomellidae currently known from Australia, their morphology is compared, their distribution within and outside Australia is noted and the high diversity of genera in southern regions emphasised. Three Australian genera are endemic, five are also found in South America, South Africa and/or New Zealand and one has a cosmopolitan distribution. The distribution, ecology and habitat preferences of Australian genera are compared. Two genera, Cassagnella Najt & Massoud and Rapoportella Ellis & Bellinger, are newly diagnosed...

Influence of prolonged dietary experience during the larval stage on novel odour preferences in adults of noctuid stem borer moths (Lepidoptera: Noctuidae)

Christophe PETIT, Peter AHUYA, Bruno LE RU, Laure KAISER-ARNAULD, Myriam HARRY, Paul-André CALATAYUD

Eur. J. Entomol. 115: 112-116, 2018 | DOI: 10.14411/eje.2018.009

In Lepidoptera, larval dietary experience of volatile cues can induce adult preference for these cues. However, such induction may require several generations, depending in part on the degree of specialization of the insects. In a previous study, using species of noctuid stem borers with different diet breadths, namely the polyphagous Sesamia nonagrioides, the oligophagous Busseola fusca and monophagous Busseola nairobica, it was shown that in S. nonagrioides, one generation was enough to induce a preference for vanillin in the resulting gravid females, whereas even two generations failed to induce a response in adults of...

The identity of figitid parasitoids (Hymenoptera: Cynipoidea: Figitidae) of anthomyiid flies in conifer cones


Eur. J. Entomol. 115: 104-111, 2018 | DOI: 10.14411/eje.2018.008

Larvae of Strobilomyia flies (Diptera: Anthomyiidae) are serious pests in conifer-seed orchards because they feed on the seed inside the cones. Figitid parasitoids (Hymenoptera: Cynipoidea) of Strobilomyia larvae in conifer cones are commonly reported but under various generic names. It is argued here that, across the entire Holarctic region, these figitids belong to Amphithectus and perhaps also to Sarothrus (Figitinae), but not to Melanips (Aspicerinae), contrary to some reports. We conclude that the identity of the commonly found figitid associated with conifer cones (Larix and Picea) in Europe and...

Revision, phylogeny and phylogeography of the cicada genus Auritibicen (Hemiptera: Cicadidae), with descriptions of ten new species


Eur. J. Entomol. 115: 53-103, 2018 | DOI: 10.14411/eje.2018.007

We review the cicada genus Auritibicen Lee, 2015 based on the description of ten new species: A. aethus sp. n., A. daoxianensis sp. n., A. pallidus sp. n., A. rotundus sp. n., A. curvatus sp. n., A. purus sp. n., A. parvus sp. n., A. gracilis sp. n., A. septatus sp. n. and A. lijiangensis sp. n. Auritibicen shikokuanus (Kato, 1959) is confirmed to be a synonym of Auritibicen kyushyuensis (Kato, 1926). Diagnoses and descriptions, along with illustrations of the structure of male genitalia, are provided for all Auritibicen species. The systematics of Auritibicen...

Loss of Y chromosome may be a synapomorphy of the tribe Lepturini (Coleoptera: Cerambycidae: Lepturinae)


Eur. J. Entomol. 115: 45-52, 2018 | DOI: 10.14411/eje.2018.006

Phylogeny and higher classification of the cerambycid subfamily Lepturinae remain controversial. Here we report the results of a cytogenetic study of 18 species currently classified in Lepturini and 12 species in other tribes of Lepturinae (1 in Oxymirini, 1 in Rhamnusiini and 10 in Rhagiini) from Western Europe. The male sex chromosome formula is XY in all Rhagiini, Oxymirini and in Grammoptera ruficornis in the Lepturini (whose tribal placement may be doubtful), and X0 in all the remaining Lepturini. The rarity of the X0 formula in other Cerambycidae indicates that the Y chromosome was lost in a common ancestor of the Lepturini or a subgroup...

Taxonomic revision of the genus Angulaphthona (Coleoptera: Chrysomelidae: Galerucinae: Alticini)


Eur. J. Entomol. 115: 30-44, 2018 | DOI: 10.14411/eje.2018.005

A revision of the genus Angulaphthona Bechyné, 1960 is provided, with a description of Angulaphthona confusa sp. n. from the Democratic Republic of Congo, and A. rossii sp. n. from Sierra Leone. The following synonymies are proposed: Angulaphthona latipennis (Pic, 1921) = A. latipennis zambeziensis (Bechyné, 1959) syn. n. and Angulaphthona pelengensis Bechyné, 1960 = A. exalta Bechyné, 1960 syn. n. The precedence of the name pelengensis is discussed. Angulaphthona violaceomicans (Chen, 1936) comb. n. (transferred from Aphthona) from Sri Lanka is established, and some...

Host-plant leaf-surface preferences of young caterpillars of three species of Pieris (Lepidoptera: Pieridae) and its effect on parasitism by the gregarious parasitoid Cotesia glomerata (Hymenoptera: Braconidae)


Eur. J. Entomol. 115: 25-29, 2018 | DOI: 10.14411/eje.2018.004

Preferences of young caterpillars of three species of Pieris (P. rapae crucivora Boisduval, P. melete Ménétriès, and P. napi japonica Shirôzu) (Lepidoptera: Pieridae) for the upper and lower surfaces of the leaves of their host plants (Brassicaceae) were investigated in the laboratory. On horseradish Armoracia rusticana Gaertn. Mey. et Scherb., which was provided as a common food for three species, second and third instar larvae of the respective species preferred the lower to the upper surface of horizontally placed leaves, irrespective of whether they hatched on the upper or lower surface. First instar larvae...

Efficiency of pitfall traps with funnels and/or roofs in capturing ground-dwelling arthropods


Eur. J. Entomol. 115: 15-24, 2018 | DOI: 10.14411/eje.2018.003

Pitfall traps are widely used for sampling ground-dwelling arthropods. Their sampling efficiency is affected by several factors, e.g. material, size and modification of parts of the trap and sampling design. Pitfall trap sampling is also affected by the accumulation of plant litter in the traps, rain fall and by-catches of small vertebrates, which may cause a bias in the catch by obstructing traps or attracting certain insects. A roof that prevents rain and plant litter entering a trap, prevents dilution of the preservative and escape of arthropods. The main goal of present study was to compare the effect of four types of differently combined funnel...

Genetic structure of populations of Aphis gossypii (Hemiptera: Aphididae) on citrus trees in Northern Iran


Eur. J. Entomol. 115: 7-14, 2018 | DOI: 10.14411/eje.2018.002

The cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae), is a serious pest of citrus in northern Iran, both because of the damaged caused by its feeding and as a vector of several viruses. The genetic structure of populations of A. gossypii on citrus trees at eight localities in Iran was surveyed using seven polymorphic microsatellite loci. Of 240 individuals tested, 142 multilocus genotypes (MLGs) were identified. The presence of multicopy genotypes and negative FIS values revealed that the major mode of reproduction in northern Iran is obligate parthenogenesis. The genotypic diversity of populations ranged between...

Puncture vs. reflex bleeding: Haemolymph composition reveals significant differences among ladybird species (Coleoptera: Coccinellidae), but not between sampling methods

Michal KNAPP, Pavel DOBE©, Michal ŘEŘICHA, Pavel HYR©L

Eur. J. Entomol. 115: 1-6, 2018 | DOI: 10.14411/eje.2018.001

Reflex bleeding is one of the many anti-predation behavioural tactics used by insects. This behaviour is recorded widely in several insect taxa and provides scientists with an interesting opportunity for easily obtaining samples of haemolymph for physiological experiments. However, there is no good evidence that haemolymph and reflex blood have the same characteristics. In this study, we compared three basic characteristics of reflex blood and haemolymph collected from the body cavity, of three species of ladybirds: Harmonia axyridis, Coccinella septempunctata and Ceratomegilla undecimnotata. In the reflex blood we recorded the...