Eur. J. Entomol. 114: 53-60, 2017 | 10.14411/eje.2017.008

The pheromone verbenone and its function in Dendroctonus armandi (Coleoptera: Curculionidae: Scolytinae)

Mingzhen ZHAO, Lulu DAI, Yaya SUN, Danyang FU, Hui CHEN*
College of Forestry, Northwest A & F University, No. 3 Taicheng Road, Yangling, Shaanxi 712100, China; e-mails: 582156006@qq.com, dailulu015@163.com, 1057893643@qq.com, 1067651606@qq.com, chenhui@nwsuaf.edu.cn

The Chinese white pine beetle, Dendroctonus armandi Tsai and Li is a native species of bark beetle and one of the most destructive in much of western China. Little is known about the characterization of the pheromones trans-verbenol and verbenone, and their functions in D. armandi are unknown. Electroantennogram tests (EAG) and olfactory assays (Y-tube assays) in the laboratory revealed that (1) trans-verbenol may be an anti-aggregation pheromone for male and an aggregation pheromone for female D. armandi and (2) female beetles are more attracted to controls (hexane) than low concentrations of verbenone and male beetles more attracted to controls than high concentrations of verbenone. Field trials indicated that the addition of verbenone to bait used to trap D. armandi remarkably decreased the efficiency of field trapping. These results indicate that verbenone is an anti-aggregation pheromone for male D. armandi. This research provides evidence of the role of verbenone among the different types of pheromones. The pheromone verbenone clearly could be used to protect healthy Chinese white pines.

Keywords: Coleoptera, Curculionidae, Dendroctonus armandi, trans-verbenol, verbenone, electrophysiological (EAG), olfactory assays (Y-tube assays), field trials

Received: September 23, 2016; Accepted: December 19, 2016; Published online: January 27, 2017

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