Eur. J. Entomol. 113: 579-586, 2016 | 10.14411/eje.2016.078

Plant volatiles challenge inhibition by structural analogs of the sex pheromone in Lobesia botrana (Lepidoptera: Tortricidae)

Albert SANS1, Miguel MORÁN2, Magí RIBA1, Ángel GUERRERO3, Jaume ROIG2, César GEMENO2
1 University of Lleida, Department of Chemistry, Av. Alcalde Rovira Roure 191, 25198 Lleida, Spain; e-mails: sans@quimica.udl.cat, mriba@quimica.udl.cat
2 University of Lleida, Department of Crop and Forest Sciences, Av. Alcalde Rovira Roure 191, 25198 Lleida, Spain; e-mails: miguel_moran1@yahoo.com.mx, jootlle@hotmail.com, cesar.gemeno@pvcf.udl.cat
3 Department of Biological Chemistry and Molecular Modelling, Institute of Advanced Chemistry of Catalonia (CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain; e-mail: angel.guerrero@iqac.csic.es

Plant volatiles can synergize the response to moth pheromone. Synthetic pheromone analogs, in turn, have the opposite effect in reducing pheromone attractiveness. To determine how these two types of stimuli interact and influence male moth behaviour, we performed wind tunnel experiments on the grapevine moth, Lobesia botrana. We noticed that a blend of host plant volatiles [(E)-β-caryophyllene, 1-hexanol, (Z)-3-hexenyl acetate and 1-octen-3-ol in a 100:20:10:5 ratio] significantly increased the response of males to an optimized blend of sex pheromone [(7E,9Z)-dodeca-7,9-dienyl acetate (E7,Z9-12:Ac), (7E,9Z)- dodeca-7,9-dienol (E7,Z9-12:OH) and (Z)-9-dodecenyl acetate (Z9-12:Ac)] in a 100:10:2 ratio. However, the response of males to the natural attractant was significantly reduced by two analogs [(9E,11Z)-tetradeca-9,11-dien-2-one (MK 2) and [(9E,11Z)-1,1,1-trifluoro-tetradeca-9,11-dien-2-one (TFMK 3)], of the major component of the sex pheromone of the insect (E7,Z9-12:Ac). When both stimuli were tested on males at pheromone:analog:plant volatile blend 1:100:1000 ratio, the plant blend offset the inhibitory effect induced by TFMK 3 but not that of MK 2. Our results show for the first time that under laboratory conditions plant volatiles can prevent inhibition by a pheromone analog.

Keywords: Lepidoptera, Tortricidae, Lobesia botrana, plant volatiles, inhibition, structural analogs, sex pheromone

Received: July 7, 2016; Accepted: November 25, 2016; Published online: December 30, 2016

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