Eur. J. Entomol. 113: 528-530, 2016 | 10.14411/eje.2016.071

Isolation and characterization of novel microsatellite loci in the genome of the dragonhead sap beetle Thymogethes norvegicus (Coleoptera: Nitidulidae)

Oddmund KLEVEN1, Anders ENDRESTØL2
1 Norwegian Institute for Nature Research (NINA), Høgskoleringen 9, NO-7034 Trondheim, Norway; e-mail: oddmund.kleven@nina.no
2 Norwegian Institute for Nature Research (NINA), Gaustadalléen 21, NO-0349 Oslo, Norway; e-mail: anders.endrestol@nina.no

Here we describe 16 novel microsatellite loci in the genome of the dragonhead sap beetle Thymogethes norvegicus, which were identified using a next-generation sequencing approach. In 40 dragonhead sap beetles from a population in southern Norway, we found 2-4 alleles per locus. Expected heterozygosity ranged from 0.18-0.65. Three of the loci showed significant deviation from Hardy-Weinberg equilibrium. After correcting for multiple tests, one pairwise locus combination displayed significant linkage disequilibrium. These novel microsatellite loci will be useful for future population structure, genetic diversity and conservation genetic studies of the dragonhead sap beetle.

Keywords: Coleoptera, Nitidulidae, Thymogethes norvegicus, microsatellite loci, genetic diversity

Received: September 12, 2016; Accepted: October 19, 2016; Published online: October 31, 2016

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References

  1. Audisio P., Cline A.R., De Biase A., Antonini G., Mancini E., Trizzino M., Costantini L., Strika S., Lamanna F. & Cerretti P. 2009: Preliminary re-examination of genus-level taxonomy of the pollen beetle subfamily Meligethinae (Coleoptera: Nitidulidae). - Acta Entomol. Mus. Nat. Pragae 49: 341-504.
  2. Easton A. 1959: A new Norwegian species of Meligethes Stephens (Col., Nitidulidae). - Norw. J. Entomol. 11: 50-53.
  3. Excoffier L. & Lischer H.E.L. 2010: Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. - Mol. Ecol. Res. 10: 564-567. Go to original source...
  4. Henriksen S. & Hilmo O. (eds) 2015: The 2015 Norwegian Red List for Species. Norwegian Biodiversity Information Centre, Trondheim, 193 pp.
  5. Rice W.R. 1989: Analyzing tables of statistical tests. - Evolution 43: 223-225. Go to original source...
  6. Rozen S. & Skaletsky H.J. 2000: Primer3 on the WWW for general users and for biologist programmers. In Krawetz S. & Kisener S. (eds): Bioinformatics Methods and Protocols: Methods in Molecular Biology. Humana Press, Totawa, NJ, pp. 365-386.
  7. Stabbetorp O.E. & Endrestøl A. 2011: Scientific Basis for Action Plan for the Northern Dragonhead Dracocephalum ruyschiana and the Northern Dragonhead Sap Beetle Meligethes norvegicus. NINA Report 766. Norwegian Institute for Nature Research, Trondheim, 61 pp.
  8. van Oosterhout C., Hutchinson W.F., Wills D.P.M. & Shipley P. 2004: MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. - Mol. Ecol. Notes 4: 535-538. Go to original source...