Eur. J. Entomol. 113: 302-306, 2016 | 10.14411/eje.2016.037

Characterization of 16 novel microsatellite loci for Ephippiger diurnus (Orthoptera: Tettigoniidae) using pyrosequencing technology and cross-species amplification

Yareli ESQUER-GARRIGOS1,2, Michael D. GREENFIELD3, Virginie PARTY3, Réjane STREIFF1,2
1 INRA, UMR 1062 CBGP, Campus International de Baillarguet, 34988 Montferrier-sur-Lez, France; e-mails: esquergarrigos@gmail.com, streiff@supagro.inra.fr
2 INRA, UMR 1333 DGIMI, Université de Montpellier, Place Eugène Bataillon, 34095 Montpellier cedex 5, France
3 Institut de Recherche sur la Biologie de l'Insecte (IRBI), CNRS UMR 7261, Parc de Grandmont, Université François Rabelais de Tours, 37200 Tours, France; e-mails: michael.greenfield@univ-tours.fr, virginie.party@univ-tours.fr

A novel panel of 16 microsatellite markers, obtained by pyrosequencing of enriched genomic libraries, is reported for the flightless European bushcricket Ephippiger diurnus (Dufour) (Orthoptera: Tettigoniidae). Five multiplex and one simplex PCR protocols were optimized, and the polymorphism at the 16 loci was assessed in two natural populations from southern France. The mean allele number and (expected mean heterozygosity) were 8.94 (0.71) and 6.57 (0.70), respectively, in each population. Several loci were at Hardy-Weinberg disequilibrium (HWD), possibly due to the incidence of null alleles. The occurrence of null alleles has been previously reported for this species, and it is a common feature of microsatellite loci in Orthoptera. Cross-amplification tests demonstrated the transferability of some of these loci to other ephippigerine species. The microsatellite loci reported here substantially increase the number of available loci for this species and will afford an accurate picture of E. diurnus phylogeography, the genetic structure of its populations, and an improved understanding of the evolution of male song and other sexually-selected traits in this highly variable species.

Keywords: Orthoptera, Tettigoniidae, Ephippiger diurnus, calling song, microsatellite polymorphism, katydid, population genetics

Received: January 22, 2016; Accepted: March 4, 2016; Published online: April 5, 2016

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