Eur. J. Entomol. 112 (2): 389-392, 2015 | DOI: 10.14411/eje.2015.047

Characterisation of sixteen additional polymorphic microsatellite loci for the spreading but locally rare European butterfly, Brenthis ino (Lepidoptera: Nymphalidae)

Christophe LEBIGRE, Camille TURLURE, Nicolas SCHTICKZELLE
Earth and Life Institute, Biodiversity Research Centre, Université catholique de Louvain, Croix du Sud 4, L7.07.04, B-1348 Louvain-la-Neuve, Belgium; e-mails: christophe.lebigre@uclouvain.be; camille.turlure@uclouvain.be; nicolas.schtickzelle@uclouvain.be

Whilst the overall geographic range of the lesser marbled fritillary, Brenthis ino (Rottemburg, 1775), is currently expanding, this species is patchily distributed at a local spatial scale due to its use of flower rich semi-natural meadows and the aggregated distribution of its host plant. Therefore, understanding the dispersal patterns of this butterfly and the effect of increasing fragmentation of the landscapes in central and Western Europe on its population dynamics is key to determining whether this patchy distribution can lead to metapopulation structuring and dynamics. One way to determine the degree to which local populations are isolated from one another is to use high resolution molecular genetic markers and thence quantify gene flow and genetic drift. Eleven microsatellite loci have previously been developed for this species, but six showed evidence of null alleles, effectively violating key assumptions of the models used to infer gene flow. We therefore developed a set of new primer pairs to amplify a suite of 16 highly polymorphic microsatellite loci (number of alleles ranged from 2-30) of which nine were found to conform to the Hardy-Weinberg's expectations, whilst at the same time not showing any clear signature for the presence of null alleles. We further describe how these primers were optimized for landscape and metapopulation genetics studies in Belgian Ardenne.

Keywords: Lepidoptera, Nymphalidae, Brenthis ino, lesser marbled fritillary, connectivity, inbreeding, landscape genetics, microsatellite, null alleles

Received: November 20, 2014; Accepted: December 28, 2014; Prepublished online: February 27, 2015; Published: April 2, 2015

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References

  1. Excoffier L., Laval G. & Schneider S. 2007: Arlequin (version 3.0): an integrated software package for population genetics data analysis. - Evol. Bioinform. Online 1: 47-50
  2. Fric Z., Hula V., Klimova M., Zimmermann K. & Konvicka M. 2010: Dispersal of four fritillary butterflies within identical landscape. - Ecol. Res. 25: 543-552 Go to original source...
  3. Goudet J. 1995: FSTAT Version 1.2: a computer program to calculate F-statistics. - J. Hered. 86: 485-486 Go to original source...
  4. Goudet J. 2001: FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available from http://www.unil.ch/izea/softwares/fstat.html. Updated from Goudet (1995)
  5. Hall L.A. & Beissinger S.R. 2014: A practical toolbox for design and analysis of landscape genetics studies. - Landsc. Ecol. 29: 1487-1504 Go to original source...
  6. Hanski I. 1999: Metapopulation Ecology. Oxford University Press, Oxford, 328 pp
  7. Malausa T., Gilles A., Meglecz E., Blanquart H., Duthoy S., Costedoat C., Dubut V., Pech N., Castagnone-Sereno P., Delye C., Feau N., Frey P., Gauthier P., Guillemaud T., Hazard L., Le Corre V., Lung-Escarmant B., Male J.P., Ferreira S. & Martin J.F. 2011: High-throughput microsatellite isolation through 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries. - Mol. Ecol. Res. 11: 638-644 Go to original source...
  8. Meglecz E., Anderson A., Bourguet D., Butcher R., Caldas A., Cassel-Lundhagen A., Coeur D'Acier A., Dawson A.D., Faure N., Fauvelot C., Franck P., Harper G., Keyghobadi N., Kluetsche C., Muthulakshmi M., Nagaraju J., Patt A., Petenian F., Silvain J.F. & Wilcock H.R. 2007: Microsatellite flanking region similarities among different loci within insect species. - Insect Mol. Biol. 16: 175-185 Go to original source...
  9. Meglecz E., Costedoat C., Dubut V., Gilles A., Malausa T., Pech N. & Martin J.-F. 2010: QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. - Bioinformatics 26: 403-404 Go to original source...
  10. Molecular Ecology Resources Primer Development Consortium 2012: Permanent genetic resources added to molecular ecology resources database 1 April 2012 - 31 May 2012. - Mol. Ecol. Resour. 12: 972-974 Go to original source...
  11. Neve G. & Meglecz E. 2000: Microsatellite frequencies in different taxa. - Trends Ecol. Evol. 15: 376-377 Go to original source...
  12. Parmesan C., Ryrholm N., Stefanescu C., Hill J.K., Thomas C.D., Descimon H., Huntley B., Kaila L., Kullberg J., Tammaru T., Tennent W.J., Thomas J.A. & Warren M. 1999: Poleward shifts in geographical ranges of butterfly species associated with regional warming. - Nature 399: 579-583 Go to original source...
  13. Raymond M. & Rousset F. 1995: GENEPOP (version 1.2): a population genetics software for exact tests and ecumenism. - J. Hered. 86: 248-249 Go to original source...
  14. Sinama M., Dubut V., Costedoat C., Gilles A., Junker M., Malausa T., Martin J.F., Neve G., Pech N., Schmitt T., Zimmermann M. & Meglecz E. 2011: Challenge for microsatellite development in Lepidoptera: Euphydryas aurinia (Nymphalidae) as a case study. - Eur. J. Entomol. 108: 261-266 Go to original source...
  15. Vandewoestijne S. & Baguette M. 2002: The genetic structure of endangered populations in the Cranberry Fritillary, Boloria aquilonaris (Lepidoptera, Nymphalidae): RAPDs vs allozymes. - Heredity 89: 439-445 Go to original source...
  16. Van Oosterhout C., Hutchinson W.F., Willis D.P.M. & Shipley P. 2004: Micro-checker: Software for identifying and correcting genotyping errors in microsatellite data. - Mol. Ecol. Notes 4: 535-538 Go to original source...
  17. Van Swaay C.A.M. & Warren M. 1999: Red Data Book of European Butterflies (Rhopalocera). Nature and Environment 99, Council of Europe Publishing, Strasbourg, 264 pp
  18. Weyer J. & Schmitt T. 2013: Knowing the way home: strong philopatry of a highly mobile insect species, Brenthis ino. - J. Insect Conserv. 17: 1197-1208 Go to original source...
  19. Zimmermann K., Fric Z., Filipova L. & Konvicka M. 2005: Adult demography, dispersal and behaviour of Brenthis ino (Lepidoptera: Nymphalidae): How to be a successful wetland butterfly. - Eur. J. Entomol. 102: 699-706 Go to original source...