Eur. J. Entomol. 112 (2): 215-226, 2015 | 10.14411/eje.2015.039

Identification of heat shock protein genes hsp70s and hsc70 and their associated mRNA expression under heat stress in insecticide-resistant and susceptible diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)

Lin Jie ZHANG1,2, Kuan Fu WANG1,2, Yu Pu JING1,2, Hua Mei ZHUANG1,2, Gang WU1,*
1 Key Laboratory of Biopesticide and Chemical Biology (Ministry of Education), Fujian Agriculture and Forestry University, 350002 Fuzhou, Fujian, China; e-mails: newugan@163.com; zljmeimei6@163.com; wkf456@yahoo.com; zhuanghuamei@yeah.net; yupujing@163.com
2 Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, 350002 Fuzhou, Fujian, China

To gain further insight into the molecular features of the ubiquitous Hsp70 family of conserved heat shock proteins, total nine full-length cDNA sequences of inducible hsp70s (Px-hsp69-1, -2a, -2b, -3, -4, Px-hsp72-1a, -1b, -2 and -3) and one constitutive hsc70 (Px-hsc70(C)) were isolated and characterized in the diamondback moth (DBM), Plutella xylostella, collected from Fuzhou, China. The nine Px-hsp70s cDNAs encoded the protein of between 629-669 amino acids with molecular weight ranging from 69.00-72.58 kDa and were derived from four hsp70 genes in the genome of DBM. The Px-hsc70(C) cDNA contained 1,953 bp of open reading frame (ORF), which produced a putative protein comprising 650 amino acids with a calculated molecular weight of 71.18 kDa. Whether in adults or larvae of chlorpyrifos-resistant (RR) and chlorpyrifos-susceptible (SS) strains of DBM, the basal level (at 25°C) of Px-hsc70(C) mRNA expression was high, but no significant up-regulation expression was found under heat stress. However, heat stress facilitated up-regulation expressions of Px-hsp70s, and SS DBM displayed higher up-regulation expression of Px-hsp70s than RR DBM. We suggest that higher up-regulation expression of Px-hsp70s in SS DBM is probably involved in their higher thermal tolerance.

Keywords: Lepidoptera, Plutellidae, Plutella xylostella, heat shock protein genes, hsp70, fitness, chlorpyrifos-resistant, chlorpyrifos-susceptible, diamondback moth

Received: September 3, 2014; Accepted: November 3, 2014; Prepublished online: February 9, 2015; Published: April 2, 2015

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