Eur. J. Entomol. 111 (5): 726-729, 2014 | 10.14411/eje.2014.084

Isolation and characterization of eleven polymorphic microsatellite markers from the beneficial carabid beetle, Poecilus cupreus (Coleoptera: Carabidae), and genetic structuring among three populations from western France

Ronan MARREC1, Stéphanie RUAULT1, Cécile RIBOUT1, Manuel PLANTEGENEST2, Bertrand GAUFFRE1, 3
1 CNRS - Université de La Rochelle, UMR 7372 CEBC, F-79360 Villiers en Bois, France; e-mails: ronanmarrec@yahoo.fr; stephanie.ruault@cebc.cnrs.fr; cecile.ribout@cebc.cnrs.fr; bertrand.gauffre@cebc.cnrs.fr
2 INRA - Agrocampus Ouest, UMR 1349 IGEPP, F-35653 Le Rheu, France; e-mail: plantage@agrocampus-ouest.fr
3 INRA, USC1339 CEBC, F-79360 Villiers en Bois, France

Poecilus cupreus (L.) (Coleoptera: Carabidae) is a typical polyphagous predator species on arable lands. In this study, 11 polymorphic microsatellite markers were developed from genomic DNA of P. cupreus. Genotypes of 87 individuals sampled from three localities in Western France were analyzed to characterize the polymorphism at each locus. The number of alleles ranged from 3 to 20. All pairs of loci were in linkage equilibrium. The expected and observed heterozygosity ranged from 0.165 to 0.890 and 0.103 to 0.926, respectively. From the northern to the southern sites, six, three, and six loci showed significant deviations from Hardy-Weinberg equilibrium, respectively. At one locus, null alleles were suspected as occuring at high frequency in the three populations. A weak genetic differentiation among populations was found, suggesting intense and large scale gene flow coupled with large population size in P. cupreus. These polymorphic microsatellite markers could be used in future population genetics studies of P. cupreus.

Keywords: Coleoptera, Carabidae, microsatellite, Poecilus cupreus, gene flow

Received: May 15, 2014; Accepted: June 30, 2014; Prepublished online: September 12, 2014; Published: December 10, 2014

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References

  1. Benjamini Y. & Hochberg Y. 1995: Controlling the false discovery rate: a practical and powerful approach to multiple testing. - J. R. Stat. Soc. (B) 57: 289-300
  2. Bommarco R. 1998: Reproduction and energy reserves of a predatory carabid beetle relative to agroecosystem complexity. - Ecol. Appl. 8: 846-853 Go to original source...
  3. Brouat C., Sennedot F., Audiot P., Leblois R. & Rasplus J.Y. 2003: Fine-scale genetic structure of two carabid species with contrasted levels of habitat specialization. - Mol. Ecol. 12: 1731-1745 Go to original source...
  4. Chapuis M.P. & Estoup A. 2007: Microsatellite null alleles and estimation of population differentiation. - Mol. Biol. Evol. 24: 621-631 Go to original source...
  5. Dempster A.P., Laird N.M. & Rubin D.B. 1977: Maximum likelihood from incomplete data via the EM algorithm. - J. R. Stat. Soc. 39: 1-38
  6. Firle S., Bommarco R., Ekbom B. & Natiello M. 1998: The influence of movement and resting behavior on the range of three carabid beetles. - Ecology 79: 2113-2122 Go to original source...
  7. Gaublomme E., Dhuyvetter H., Verdyck P., Mondor-Genson G., Rasplus J.-Y. & Desender K. 2003: Isolation and characterization of microsatellite loci in the ground beetle Carabus problematicus (Coleoptera, Carabidae). - Mol. Ecol. Notes 3: 341-343 Go to original source...
  8. Gaublomme E., Maebe K., van Doninck K., Dhuyvetter H., Li X., Desender K. & Hendrickx F. 2013: Loss of genetic diversity and increased genetic structuring in response to forest area reduction in a ground dwelling insect: a case study of the flightless carabid beetle Carabus problematicus (Coleoptera, Carabidae). - Insect Cons. Div. 6: 473-482 Go to original source...
  9. Hendrickx F., Maelfait J.P., Desender K., Aviron S., Bailey D., Diekotter T., Lens L., Liira J., Schweiger O. & Speelmans M. 2009: Pervasive effects of dispersal limitation on within-and among-community species richness in agricultural landscapes. - Global Ecol. Biogeogr. 18: 607-616 Go to original source...
  10. Holland J.M. & Luff M.L. 2000: The effects of agricultural practices on Carabidae in temperate agroecosystems. - Integr. Pest Manag. Rev. 5: 109-129 Go to original source...
  11. Holland J.M., Thomas C.F.G., Birkett T., Southway S. & Oaten H. 2005: Farm-scale spatiotemporal dynamics of predatory beetles in arable crops. - J Appl. Ecol. 42: 1140-1152 Go to original source...
  12. Jeannel R. 1942: Coleopteres Carabiques (2e partie). Faune de France 40. Lechevalier, Paris, 600 pp
  13. Keller I., Excoffier L. & Largiader C.R. 2005: Estimation of effective population size and detection of a recent population decline coinciding with habitat fragmentation in a ground beetle. - J. Evol. Biol. 18: 90-100 Go to original source...
  14. Lagisz M., Wolff K., Sanderson R.A. & Laskowski R. 2010: Genetic population structure of the ground beetle, Pterostichus oblongopunctatus, inhabiting a fragmented and polluted landscape: Evidence for sex-biased dispersal. - J. Insect Sci. 10: 105 Go to original source...
  15. Langmaack M., Land S. & Buchs W. 2001: Effects of different field management systems on the carabid coenosis in oil seed rape with special respect to ecology and nutritional status of predacious Poecilus cupreus L. (Col., Carabidae). - J. Appl. Entomol. 125: 313-320 Go to original source...
  16. Losey J.E. & Vaughan M. 2006: The economic value of ecological services provided by insects. - BioScience 56: 311-323 Go to original source...
  17. Malausa T., Gilles A., Meglecz E., Blanquart H., Duthoy S., Costedoat C., Dubut V., Pech N., Castagnone-Sereno P. & Delye C. 2011: High-throughput microsatellite isolation through 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries. - Mol. Ecol. Res. 11: 638-644 Go to original source...
  18. Marchi C., Andersen L.W. & Loeschcke V. 2013: Effects of land management strategies on the dispersal pattern of a beneficial arthropod. - PloS One 8: e66208 Go to original source...
  19. Marcus T., Assmann T., Durka W. & Drees C. 2013: A suite of multiplexed microsatellite loci for the ground beetle Abax parallelepipedus (Piller and Mitterpacher, 1783) (Coleoptera, Carabidae). - Conserv. Genet. Resour. 5: 1151-1156 Go to original source...
  20. Matern A., Desender K., Drees C., Gaublomme E., Paill W. & Assmann T. 2009: Genetic diversity and population structure of the endangered insect species Carabus variolosus in its western distribution range: Implications for conservation. - Conserv. Genet. 10: 391-405 Go to original source...
  21. Nei N. 1987: Molecular Evolutionary Genetics. Columbia University Press, New York, 512 pp
  22. Rousset F. 2008: Genepop 007: a complete re-implementation of the Genepop software for Windows and Linux. - Mol. Ecol. Res. 8: 103-106 Go to original source...
  23. Rutkowski R., Szczuka A., Zalewski M., Korczynska J. & Gryziak G. 2011: Failure of microsatellite s cross-species amplification in common ground beetle Pterostichus melanarius (Illiger). - Baltic J. Coleopterol. 11: 17-24
  24. Sander A.C., Purtauf T., Holzhauer S.I.J. & Wolters V. 2006a: Landscape effects on the genetic structure of the ground beetle Poecilus versicolor Sturm 1824. - Biodiv. Conserv. 15: 245-259 Go to original source...
  25. Sander A.C., Purtauf T., Wolters V. & Dauber J. 2006b: Landscape genetics of the widespread ground-beetle Carabus auratus in an agricultural region. - Basic Appl. Ecol. 7: 555-564 Go to original source...
  26. Skuhravy V. 1957: Bewegungsareal einiger Carabidenarten. - Acta Soc. Entomol. Bohemoslov. 53: 171-179 [in Czech, Russian and German abstr.]
  27. Thiele H.U. 1977: Carabid Beetles in their Environments. A Study on Habitat Selection by Adaptation in Physiology and Behaviour. Springer, Berlin, 369 pp
  28. van Oosterhout C., Hutchinson W.F., Wills D.P.M. & Shipley P. 2004: Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. - Mol. Ecol. Notes 4: 535-538 Go to original source...
  29. van Oosterhout C., Weetman D. & Hutchinson W.F. 2006: Estimation and adjustment of microsatellite null alleles in nonequilibrium populations. - Mol. Ecol. Res. 6: 255-256 Go to original source...
  30. Wallin H. 1985: Spatial and temporal distribution of some abundant carabid beetles (Coleoptera: Carabidae) in cereal fields and adjacent habitats. - Pedobiologia 28: 19-34
  31. Weir B.S. & Cockerham C.C. 1984: Estimating F-statistics for the analysis of population structure. - Evolution 38: 1358-1370 Go to original source...
  32. Westerberg L., Ostman O. & Wennergren U. 2005: Movement effects on equilibrium distributions of habitat generalists in heterogeneous landscapes. - Ecol. Model. 188: 432-447 Go to original source...
  33. Wilson A.C.C., Massonnet B., Simon J.-C., Prunier-Leterme N., Dolatti L., Llewellyn K.S., Figueroa C.C., Ramirez C.C., Blackman R.L., Estoup A. & Sunnucks P. 2004: Cross-species amplification of microsatellite loci in aphids: assessment and application. - Mol. Ecol. Notes 4: 104-109 Go to original source...