Eur. J. Entomol. 111 (4): 453-456, 2014 | 10.14411/eje.2014.059

Sex ratio distortion in the Nesolynx thymus (Hymenoptera: Eulophidae), an ecto-pupal parasitoid of uzifly, Exorista sorbillans (Diptera: Tachinidae)

Bandekodigenahalli M. PRAKASH1, Aswathaiah PRATHIMA2, Hoolageri C. HUCHESH2, Hemagirigowda RAVIKUMAR2, Shankaranarayan SAMPATHKUMAR2, Hosagavi P. PUTTARAJU2
1 Evolutionary Biology Laboratory, Evolutionary and Organismal Biology Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur PO, Bangalore 560064, Karnataka, India; e-mail: prakashbm@gmail.com
2 Department of Biological Sciences, Bangalore University, Bangalore 560056, Karnataka, India; e-mails: prathima2805@gmail.com; hucheshbt@gmail.com; ravikumarh79@gmail.com; sam82kumar@gmail.com; puttarajuhp@hotmail.com

The reproductive alterations induced by maternally inherited α-proteo-bacteria Wolbachia to their hosts is a well-documented phenomenon. In Nesolynx thymus, a gregarious hymenopterous ecto-pupal parasitoid of the uzifly, Exorista sorbillans, diagnostic PCR assay using specific primers revealed the presence of Wolbachia. Following genetic crossing experiments, we observed a female biased sex ratio of 1 : 9.5 at 25°C and 1 : 3 male to female ratio when the populations were exposed to heat shock 33°C for six hours. Furthermore, we found infection polymorphism, where female parasitoids are infected by Wolbachia but males are not infected. Infected eggs develop into females, whereas uninfected eggs develop parthenogenetically into males. The results are discussed in the context of the possible mechanism of sex-ratio bias caused by Wolbachia.

Keywords: Hymenoptera, Eulophidae, Diptera, Tachinidae, Wolbachia, Nesolynx thymus, Exorista sorbillans, sex ratio distortion, parthenogenesis, temperature treatment

Received: December 25, 2013; Accepted: April 22, 2014; Prepublished online: August 13, 2014; Published: October 1, 2014

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