Eur. J. Entomol. 111 (3): 443-447, 2014 | DOI: 10.14411/eje.2014.043

Rearing of Microplitis mediator (Hymenoptera: Braconidae) and its host Mamestra brassicae (Lepidoptera: Noctuidae)

Elodie BELZ1,2, Céline E. GÉNEAU1,3, Moritz FÜRST1,4, Oliver BALMER1,5,*, Pius ANDERMATT6, Lukas PFIFFNER1, Léon WESTERD7, Henryk LUKA1,8
1 Department of Plant Protection and Biodiversity, Research Institute of Organic Agriculture (FiBL), Ackerstrasse 21, Postfach 219, 5070 Frick, Switzerland; e-mails: oliver.balmer@unibas.ch; oliver.balmer@aya.yale.edu; elodie.belz@live.fr; celine.geneau@gmail.com; moritz.fuerst@fibl.org; henryk.luka@fibl.org
2 Zoological Institute, Department of Environmental Sciences, Faculty of Science, University of Basel, Vesalgasse 1, 4051 Basel, Switzerland
3 Centre for Sustainable Agriculture, Lancaster Environment Centre, Lancaster University, 10 Lancaster LAI 4YQ, UK
4 Fachmaturitätsschule (FMS) Basel-Stadt, Engelgasse 120, 4052 Basel, Switzerland
5 Swiss Tropical and Public Health Institute (Swiss TPH), Socinstrasse 57, 4051 Basel, Switzerland
6 Technical Insect Rearing, Syngenta Crop. Protection Münchwilen AG, Breitenloh 5, 4333 Münchwilen, Switzerland
7 Laboratory of Entomology, Wageningen University, P.O. Box 8031, 6700 EH Wageningen, The Netherlands
8 Department of Environmental Sciences, NLU-Biogeography, University of Basel, St. Johanns-Vorstadt 10, 4056 Basel, Switzerland

Establishing continuous and reliable colonies of pest-parasitoid systems in the laboratory is an essential requirement for carrying out manipulative experiments on biological control. Here we describe in detail the rearing protocols that we optimized for the efficient rearing of the cabbage moth Mamestra brassicae and its key parasitoid Microplitis mediator.

Keywords: Hymenoptera, Braconidae, Microplitis mediator, Lepidoptera, Noctuidae, Mamestra brassicae, pest, parasitoid, cabbage moth, protocol

Received: October 30, 2013; Accepted: January 6, 2014; Prepublished online: May 6, 2014; Published: July 14, 2014

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