Eur. J. Entomol. 110 (3): 541-543, 2013 | 10.14411/eje.2013.072

Isolation and characterization of microsatellite loci for the mud-dauber wasp Trypoxylon (Trypargilum) albitarse (Hymenoptera: Crabronidae)

Juliano DA COSTA ALMEIDA1, Antonio Carlos BRAGATO BERGAMASCHI1, Alexandra SANCHES2, Terumi HATANAKA1, Marco Antonio DEL LAMA1
1 Departamento de Genética e Evolução, Universidade Federal de São Carlos, 13565-905, São Carlos, SP, Brazil; e-mails: juliano03@gmail.com; caito_bio@yahoo.com.br; hterumi@yahoo.com.br; dmdl@ufscar.br
2 Departamento de Ecologia, Universidade Estadual Paulista, 13506-900, Rio Claro, SP, Brazil; e-mail: lelesanches@gmail.com

This paper describes the characterization of a set of nine microsatellite markers for the pipe-organ mud-dauber wasp, Trypoxylon albitarse F. (Hymenoptera: Crabronidae), a common solitary wasp species in Brazil. Eight of the nine loci described were found to be polymorphic. The number of alleles ranged from three to 11. Observed and expected heterozygosity on the population level ranged from 0.135 to 0.891 and 0.177 to 0.865, respectively. Genotypic analyses revealed no deviations from Hardy-Weinberg equilibrium or linkage disequilibrium after sequential Bonferroni correction. Cross-species amplification was also tested for nine related species and positive amplifications were found in most of the assays. These markers will be useful in studies involving relatedness analysis and investigations of the population genetics of these wasps.

Keywords: Hymenoptera, Crabronidae, Trypoxylon albitarse, microsatellites, genetic characterization

Received: December 4, 2012; Accepted: December 20, 2012; Published: July 11, 2013

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