Eur. J. Entomol. 110 (3): 427-433, 2013 | 10.14411/eje.2013.057

Comparative study of mtDNA in species of the genus Adalia (Coleoptera: Cocinellidae) and origin of ancient mitochondrial haplotypes in the gene pool of Adalia bipunctata

Ilya ZAKHAROV, Elena SHAIKEVICH
N.I. Vavilov Institute of General Genetics, 3 ul. Gubkina, Moscow 119991, Russia; e-mails: iaz34@mail.ru; elenashaikevich@mail.ru

Fifteen different mitochondrial haplotypes of the mtDNA gene COI encoding cytochrome C oxidase subunit I were identified in the 127 individuals of Adalia bipunctata studied. Two mitochondrial haplotypes, H9 and H10, differed greatly from the others. The mitochondrial polymorphism in A. bipunctata is ancient, though its age remains to be evaluated. It is shown that mitochondrial haplotypes H9 and H10 and others coexisted in the original population of A. bipunctata before it spread throughout Eurasia from Western Europe to the Baikal Area, and before the differentiation of the subspecies A. bipunctata fasciatopunctata, which differs from the European form in its elytral pattern. In order to evaluate the possible origin of the ancient mitochondrial haplotypes in the gene pool of A. bipunctata sequences of the mtDNA gene COI and of the rRNA second internal transcribed spacer of the four species of Adalia: A. bipunctata, A. decempunctata, A. frigida and A. tetraspilota, were compared. It is suggested that infection with Rickettsia had an important role in the preservation of the mitochondrial haplotypes H9 and H10 during the evolution of Adalia.

Keywords: Coleoptera, Cocinellidae, Adalia bipunctata, COI, ITS2, Rickettsia

Received: April 3, 2012; Accepted: November 15, 2012; Published: July 11, 2013

Download citation

References

  1. DYER K.A., BURKE C. & JAENIKE J. 2011: Wolbachia-mediated persistence of mtDNA from a potentially extinct species. - Mol. Ecol. 20: 2805-2817 Go to original source...
  2. FOLMER O., BLACK M., HOEH W., LUTZ R. & VRIJENHOEK R. 1994: DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. - Mol. Mar. Biol. Biotechnol. 3: 294-299
  3. HAAG-LIAUTARD C., CROWN N., HOULE D., LYNCH M., CHARLESWORTH B. & KEIGHTLEY P.D. 2008: Direct estimation of the mitochondrial DNA mutation rate in D. melanogaster. - PLoS Biol. 6: 1706-1714 Go to original source...
  4. HURST G.D.D., JIGGINS F.M., SCHULENBERG J.H.G., BERTRAND D., WEST S.A., GORIACHEVA I.I., ZAKHAROV I.A., WERREN J.H., STOUTHAMER R. & MAJERUS M.E.N. 1999: Male-killing Wolbachia in two species of insect. - Proc. R. Soc. Lond. (B) 266: 735-740 Go to original source...
  5. JIGGINS F.M. & TINSLEY M.C. 2005: An ancient mitochondrial polymorphism in Adalia bipunctata linked to a sex-ratiodistorting bacterium. - Genetics 171: 1115-1124 Go to original source...
  6. KARTAVTSEV YU.P. 2011: Sequence divergence at mitochondrial genes in animals: applicability of DNA data in genetics of speciation and molecular phylogenetics. - Mar. Genom. 4: 71-81 Go to original source...
  7. LIBRADO P. & ROZAS J. 2009: DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. - Bioinformatics 25: 1451-1452 Go to original source...
  8. LUS YA.YA. 1928: On the inheritance of color and pattern in lady beetles Adalia bipunctata L. and Adalia decempunctata L. - Izv. Byuro Genet. (Leningrad) 6: 89-163 [in Russian, English abstr.]
  9. LUS YA.YA. 1932: An analysis of the dominance phenomenon in the inheritance of the elytra and pronotum colour in Adalia bipunctata. - Trudy Lab. Genet. (Leningrad) 9: 135-162
  10. LUS YAYA. 1947: Some trends in reproduction of populations of Adalia bipunctata L. maleless lines in populations. - Reports USSR Acad. Sci. 57: 951-954 [in Russian, English abstr.]
  11. LUSIS (LUS) YA.YA. 1973: Taxonomic relations and geographic distribution of ladybirds of the genus Adalia Mulsant. - Proc. Latvian State Univ. 184: 5-128 [in Russian, English abstr.]
  12. LUSIS (LUS) YA.YA. 1976: [On taxonomy of Adalia frigida Schneider (Coleoptera, Coccinellidae)]. - Genetic and Selectional Studies in Lat. SSR. - Proc. of the Conf. Riga, Zinatne. pp. 3-6 [in Russian]
  13. MAJERUS M.E.N. 1994: Ladybirds. Harper Collins, London, 366 pp
  14. MARIN J., CROUAU-ROY B., HEMPTINNE J.-L., LECOMPTE E. & MAGRO A. 2010: Coccinella septempunctata (Coleoptera, Coccinellidae): a species complex? - Zool. Scr. 35: 591-602 Go to original source...
  15. PORTER C.H. & COLLINS F.H. 1991: Species-diagnostic differences in a ribosomal DNA internal transcribed spacer from the sibling species Anopheles freeborni and Anopheles hermsi (Diptera: Culicidae). - Am. J. Trop. Med. Hyg. 45: 271-279
  16. SHAIKEVICH E.V., IVSHINA E.V. & ZAKHAROV I.A. 2012: Polymorphism of mtDNA and distribution of cytoplasmic symbionts in populations of the two-spot ladybird beetle Adalia bipunctata. - Russ. J. Genet. 48: 566-570 Go to original source...
  17. SMITH O.J. 1953: Species, distribution, and host records of the braconid genera Microctonus and Perilitus (Hymenoptera: Braconidae). - Ohio J. Sci. 53: 173-178
  18. SCHULENBURG J.H. VON DER, HABIG M., SLOGGETT J.J., WEBBERLEY K.M., BERTRAND D., HURST G.D. & MAJERUS M.E.N. 2001: Incidence of male-killing Rickettsia spp. (a-Proteobacteria) in the ten-spot ladybird beetle Adalia decempunctata L. (Coleoptera: Coccinellidae). - Appl. Environ. Microbiol. 67: 270-277 Go to original source...
  19. SCHULENBURG J.H., HURST G.D., TETZLAFF D., BOOTH G.E., ZAKHAROV I.A. & MAJERUS M.E.N. 2002: History of infection with different male-killing bacteria in the two-spot ladybird beetle Adalia bipunctata revealed through mitochondrial DNA sequence analysis. - Genetics 160: 1075-1086
  20. TAMURA K., DUDLEY J., NEI M. & KUMAR S. 2007: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. - Mol. Biol. Evol. 24: 1596-1599 Go to original source...
  21. WINGO C.W. 1952: The Coccinellidae (Coleoptera) of the upper Mississippi basin. - Iowa State J. Sci. 27: 15-53