Eur. J. Entomol. 110 (3): 411-418, 2013 | 10.14411/eje.2013.055

Chromosomal location of rDNA clusters and TTAGG telomeric repeats in eight species of the spittlebug genus Philaenus (Hemiptera: Auchenorrhyncha: Aphrophoridae)

Anna MARYAŃSKA-NADACHOWSKA1, Valentina G. KUZNETSOVA2, Tatyana V. KARAMYSHEVA3
1 Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Kraków, Poland; e-mail: maryanska@isez.pan.krakow.pl
2 Zoological Institute of the Russian Academy of Sciences, Saint-Petersburg, Russia; e-mail: karyo@zin.ru
3 Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; e-mail: kary@bionet.nsc.ru

A cytogenetic investigation was performed in eight species of the spittlebug genus Philaenus using silver-NOR (AgNOR)-banding and fluorescence in situ hybridization (FISH) with 18S rDNA and (TTAGG)n telomeric probes. This is the first application of FISH technique in the Auchenorrhyncha, a suborder of the Hemiptera. FISH along with the rDNA probe revealed differences between species in the number and chromosomal location of major ribosomal RNA gene sites, the so-called nucleolar organizer regions (NORs). However, we found a lack of perfect correlation between the results of AgNOR-staining and rDNA-FISH in the detection of NORs. FISH with the telomeric probe confirmed that the chromosome ends of the Philaenus species are composed of the (TTAGG)n nucleotide sequence, which is a common motif of insect telomeres.

Keywords: Philaenus, Aphrophoridae, Auchenorrhyncha, FISH, Ag-NOR, rDNA, telomeres

Received: August 29, 2012; Accepted: March 29, 2013; Published: July 11, 2013

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