Eur. J. Entomol. 110 (2): 383-387, 2013 | 10.14411/eje.2013.098

Characterization of ten polymorphic microsatellite markers for an endangered butterfly Argynnis niobe and their cross-species utility in the closely related species A. adippe (Lepidoptera: Nymphalidae)

Jan ZIMA Jr.1,2, Dan LEŠTINA1, Martin KONVIČKA1,2
1 Faculty of Sciences, Department of Zoology, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic; e-mails: zimapanz@seznam.cz, dan.lestina@gmail.com, konva333@gmail.com
2 Biology Centre AS CR, Institute of Entomology, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic

The Niobe Fritillary, Argynnis niobe, is a habitat specialist and as a consequence is highly endangered in contemporary Europe. To investigate its genetic diversity and population structure, 10 polymorphic microsatellite loci were developed and characterized, using a recently developed pyrosequencing method. The number of alleles per locus ranged from 2 to 21, and the observed and expected heterozygosities varied from 0.17 to 0.53 and from 0.24 to 0.92, respectively. These loci were also successfully used to study the genetic diversity of a closely related species, the High Brown Fritillary, Argynnis adippe, and will be used in future population structure studies of both these species.

Keywords: Carpathian Mts, conservation genetics, Lepidoptera, Nymphalidae, Argynnis niobe, A. adippe

Published: April 11, 2013

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