Eur. J. Entomol. 110 (2): 263-270, 2013 | 10.14411/eje.2013.039

Cold storage affects mortality, body mass, lifespan, reproduction and flight capacity of Praon volucre (Hymenoptera: Braconidae)

Juracy Caldeira LINS Jr.1, Vanda Helena Paes BUENO*,1, Livia Alvarenga SIDNEY1, Diego Bastos SILVA1, Marcus Vinicius SAMPAIO2, Janser Moura PEREIRA3, Quintiliano Siqueira Schroden NOMELINI3, Joop C. van LENTEREN4
1 Department of Entomology, Federal University of Lavras, CP 3037, MG 37200-000, Lavras, Brazil; e-mails: juracylins@yahoo.com.br; vhpbueno@den.ufla.br; liviasidney@yahoo.com.br
2 Agricultural Sciences Institute, Federal University of Uberlandia, MG 38400-902, Uberlandia, Brazil; e-mail: mvsampaio@iciag.ufu.br
3 Faculty of Mathematics, Federal University of Uberlandia, MG 38408-100, Uberlandia, Brazil
4 Laboratory of Entomology, Wageningen University, Wageningen, The Netherlands; e-mail: Joop.vanLenteren@wur.nl

The possibility of storing natural enemies at low temperatures is important for the mass production of biological control agents. We evaluated the effect of different periods of cold storage on immature mortality, mummy body mass, lifespan, reproduction and flight capacity of the parasitoid Praon volucre (Haliday). One-day-old mummies of the aphid Macrosiphum euphorbiae (Thomas) containing pre-pupae of P. volucre were stored in a climatic chamber at 5C and 70 ± 10% RH in the dark for different periods of time (5, 10, 15 and 20 days). The control consisted of mummies kept at 22 ± 1C, 70 ± 10% RH and a 12 h photophase. Percentage adult emergence, mummy body mass, flight capacity and number of eggs in the ovarioles of P. volucre females decreased with increase in the period of storage, while the longevity of females was only slightly affected. Fat content of mummies, percentage of parasitized aphids and survival of progeny to emergence decreased with increase in the period of storage. Storage of P. volucre pre-pupae for up to 5 days at 5C did not affect any of the above mentioned parameters. The fact that P. volucre pre-pupae can be stored for 5 days without loss of quality and for 10 days with only a slight loss facilitates the planning of mass production and shipment.

Keywords: Hymenoptera, Braconidae, Praon volucre, parasitoid quality, biological control, storage, Macrosiphum euphorbiae

Received: August 15, 2012; Accepted: October 23, 2012; Published: April 11, 2013

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