Eur. J. Entomol. 110 (2): 247-252, 2013 | DOI: 10.14411/eje.2013.037
Behavioural and toxicological responses of Blattella germanica (Dictyoptera: Blattellidae) to monoterpenes
- 1 Centro de Investigaciones de Plagas e Insecticidas (CIPEIN-CITEDEF/CONICET), J.B. de La Salle 4397, (1603) Villa Martelli, Prov. de Buenos Aires, Argentina; e-mails: ralzogaray@hotmail.com; vsfara@citedef.gob.ar; amoretti@citedef.gob.ar; ezerba@citedef.gob.ar
- 2 Instituto de Investigación e Ingeniería Ambiental, Universidad Nacional de San Martín, San Martín, Prov. de Buenos Aires, Argentina
Certain monoterpenes produced by aromatic plants are known to have lethal and sublethal effects on insects. As there is a need to replace conventional pesticides (organophosphates, carbamates, pyrethroids) with biorational pesticides, we evaluated the behavioural and toxicological responses of the German cockroach, Blattella germanica, exposed to the following monoterpenes, namely (+)-a-pinene, (-)-a-pinene, limonene, menthone, linalool, menthyl acetate and geraniol. Locomotor activity and repellency were quantified using a video tracking system. To study the effect on locomotor activity, larvae were exposed to filter papers treated with concentrations of monoterpenes ranging between 1.4 and 1,400 µg/cm2. Only menthyl acetate applied at a concentration of 14 µg/cm2 or higher increased the locomotor activity of the larvae. Repellency was evaluated by exposing larvae to circles of filter paper divided in two equal zones: one zone was treated with a solution of a monoterpene in acetone (14 or 140 µg/cm2) and the other with only acetone. Larvae were repelled by all the monoterpenes tested. The two concentrations of geraniol and the highest concentration of menthyl acetate and linalool were as repellent as DEET (positive control). The fumigant effect was evaluated by exposing larvae to vapour from 100 µl of pure monoterpene in a sealed container. The values of the 50% Knock-down Times (KT50), expressed in minutes, were: (+)-a-pinene: 11.8, (-)-a-pinene: 14.6, limonene: 81.0, menthone: 141.0, and linalool: 238.6. (+)-a-Pinene was as good a fumigant as dichlorvos (positive control, KT50 = 9.7 min). The least effective compounds were menthyl acetate and geraniol (both affected less than 50% of larvae after 490 min of exposure). In conclusion, (+)-a-pinene (for its fumigant effect), menthyl acetate (for its hyperactivant effect) and menthone and geraniol (for their repellent properties) could potentially be used for controlling B. germanica.
Keywords: Dictyoptera, Blattellidae, Blattella germanica, German cockroach, locomotor activity, monoterpenes, repellency, knock-down
Received: April 10, 2012; Accepted: October 1, 2012; Revised: October 1, 2012; Published: April 11, 2013Show citation
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References
- ALZOGARAY R.A. & ZERBA E.N. 2001: Third instar nymphs of Rhodnius prolixus exposed to α-cyanopyrethroids: from hyperactivity to death. - Arch. Insect Biochem. Physiol. 46: 119-126
Go to original source...
- ALZOGARAY R.A., FONTAN A. & ZERBA E.N. 1997: Evaluation of hyperactivity produced by pyrethroid treatment on third instar nymphs of Triatoma infestans (Hemiptera: Reduviidae). - Arch. Insect Biochem. Physiol. 35: 323-333
Go to original source...
- ALZOGARAY R.A., FONTAN A. & ZERBA E.N. 2000: Repellency of DEET to nymphs of Triatoma infestans. - Med. Vet. Entomol. 14: 6-10
Go to original source...
- ALZOGARAY R.A., LUCIA A., ZERBA E.N. & MASUH H.M. 2011: Insecticidal activity of essential oils from eleven Eucalyptus spp. and two hybrids: Lethal and sublethal effects of their major components on Blattella germanica. - J. Econ. Entomol. 104: 595-600
Go to original source...
- BINUKUMAR B.K. & GILL K.D. 2010: Cellular and molecular mechanisms of dichlorvos neurotoxicity: cholinergic, noncholinergic, cell signaling, gene expression and therapeutic aspects. - Indian J. Exp. Biol.48: 697-709
- BRENNER R.J. 1995: Medical and economic significance. In Rust M.K., Owens J.M. & Reierson D.A. (eds): Understanding and Controlling the German Cockroach. Oxford University Press, Oxford, pp. 77-92
- CHEN W. & VILJOEN A.M. 2010: Geraniol - A review of a commercially important fragrance material. - S. Afr. J. Bot. 76: 643-651
Go to original source...
- CHOI W., PARK B., KU S. & LEE S. 2002: Repellent activities of essential oils and monoterpenes against Culex pipiens pallens. - J. Am. Mosq. Control Assoc. 18: 348-351
- COCHRAN D.G. 2003: Blattodea. In Resh V.H. & Carde R.T. (eds): Encyclopedia of Insects. Academic Press, New York, pp. 123-126
- CORNELIUS M.L., GRACE J.K. & YATES J.R. 1997: Toxicity of monoterpenoids and other natural products to the Formosan subterranean termite (Isoptera: Rhinotermitidae). - J. Econ. Entomol. 90: 320-325
Go to original source...
- DETHIER V.G., BROWNE L.B. & SMITH C.N. 1960: The designation of chemicals in terms of the responses they elicit from insects. - J. Econ. Entomol. 53: 134-136
Go to original source...
- DOWLING K.C. & SEIBER J.N. 2002: Importance of respiratory exposure to pesticides among agricultural. - Int. J. Toxicol. 21: 371-381
Go to original source...
- ENAN E.E. 2001: Insecticidal activity of essential oils: octopaminergic sites of action. - Comp. Biochem. Physiol. (C) 130: 325-337
- ENAN E.E. 2005: Molecular and pharmacological analysis of an octopamine receptor from American cockroach and fruit fly in response to plant essential oils. - Arch. Insect Biochem. Physiol. 59: 161-171
Go to original source...
- ESPINOSA DIAZ M.A., GUETACHEW T., LANDY P., JOSE J. & VOILLEY A. 1999: Experimental and estimated saturated vapour pressures of aroma compounds. - Fluid Phase Equilib. 157: 257-270
Go to original source...
- GAMMON D.W. 1978: Neural effects of allethrin on the free walking cockroach Periplaneta americana: an investigation using defined doses at 15 and 32°C. - Pestic. Sci. 9: 79-91
Go to original source...
- GUALTIERI J.M., NELSON M. & CICHERO J.A. 1985: Presente y perspectiva del control quimico. In Carcavallo R.U., Rabinovich J.E. & Tonn R.J. (eds): Factores Biologicos y Ecologicos en la Enfermedad de Chagas. Centro Panamericano de Ecologia Humana y Salud, Organizacion Panamericana de la Salud, Servicio Nacional de Chagas y Ministerio de Salud y Accion Social de la Republica Argentina, pp. 319-329
- HUMMELBRUNNER L.A. & ISMAN M.B. 2001: Acute, sublethal, antifeedant, and synergistic effects of monoterpenoid essential oil compounds on the tobacco cutworm, Spodoptera litura (Lep., Noctuidae). J. Agric. Food Chem. 49: 715-720
Go to original source...
- ISMAN M.B. 2000: Plant essential oil for pest and disease management. - Crop Prot. 19: 603-608
Go to original source...
- ISMAN M.B. 2006: The role of botanical insecticides, deterrents and repellents in modern agriculture and an increasingly regulated world. - Annu. Rev. Entomol. 51: 45-66
Go to original source...
- ISMAN M.B. 2010: Botanical insecticides, deterrents, repellents and oils. In Singh B.P. (ed.): Industrial Crops and Uses. CABI, Oxfordshire, pp. 433-445
Go to original source...
- ISMAN M.B. & AKHTAR Y. 2007: Plant natural products as a source for developing environmentally acceptable insecticides. In Ishaaya I., Nauen R. & Horowitz A.R. (eds): Insecticides Design Using Advanced Technologies. Springer, Berlin, pp. 235-248
- ISMAN M.B. & MACHIAL C.M. 2006: Pesticides based on plant essential oils: from traditional practice to commercialization In Rai M. & Carpinella M.C. (eds): Naturally Occurring Bioactive Compounds. Advances in Phytomedicine, Vol. 3. Elsevier, London, pp. 29-44.
- ISMAN M.B. & PALUCH G. 2011: Needles in the haystack: exploring chemical diversity of botanical insecticides. In Lopez O. & Fernandez-Bolanos J.G. (eds): Green Trends in Insect Control. Royal Society of Chemistry, Cambridge, London, pp. 248-265
- ISMAN M.B., MACHIAL C.M., MIRESMAILLI S. & BAINARD L.D. 2007: Essential oil-based pesticides: new insights from old chemistry. In Ohkawa H., Miyagawa H. & Lee P. (eds): Pesticide Chemistry. Wiley, Weinheim, pp. 201-209
- JANG Y.-S., YANG Y.-C., CHOI D.-S. & AHN Y.-J. 2005: Vapor phase toxicity of marjoram oil compounds and their related monoterpenoids to Blattella germanica (Orthoptera: Blattellidae). - J. Agric. Food Chem. 53: 7892-7898
Go to original source...
- KARR L.L., DREWES C.D. & COATS J.R. 1990: Toxic effects of d-limonene in the earthworm Eisenia fetida (Savigny). - Pest. Biochem. Physiol. 36: 175-186
Go to original source...
- KATZ T.M., MILLER J.H. & HEBERT A.A. 2008: Insect repellents: Historical perspectives and new developments. - J. Am. Acad. Dermatol. 58: 865-871
Go to original source...
- LEE S., PETERSON C.J. & COATS J.R. 2003: Fumigation toxicity of monoterpenoids to several stored product insects. - J. Stored Prod. Res. 39: 77-85
Go to original source...
- LOPEZ M.D. & PASCUAL-VILLALOBOS M.J. 2010: Mode of inhibition of acetylcholinesterase by monoterpenoids and implications for pest control. - Ind. Crop. Prod. 31: 284-288
Go to original source...
- LUCIA A., GONZALEZ AUDINO P., SECCACINI E., LICASTRO S., ZERBA E. & MASUH H. 2007: Larvicidal effect of Eucalyptus grandis essential oil and turpentine and their major components on Aedes aegypti larvae. - J. Am. Mosq. Control Assoc. 23: 299-303
Go to original source...
- LUCIA A., LICASTRO S., ZERBA E., GONZALEZ AUDINO P. & MASUH H. 2009: Sensitivity of Aedes aegypti adults (Diptera: Culicidae) to the vapors of Eucalyptus essential oils. - Bioresour. Technol. 100: 6083-6087
Go to original source...
- LYMAN W.J. 1985: Estimation of physical properties. In Neely W.B. & Blau G.E. (eds): Environmental Exposure From Chemicals. Vol. 1. CRC Press, Boca Raton, pp. 13-47
- MILLER T.A. & ADAMS M.E. 1982: Mode of action of pyrethroids. In Coats J.R. (ed.): Insecticide Mode of Action. Academic Press, New York, pp. 3-27
- MINISTERIO DE SALUD 2011: Quimicos prohibidos y restringidos en Argentina [Chemicals Banned and Restricted in Argentina.] Ministerio de Salud de la Nacion Argentina, Buenos Aires, 19 pp. [in Spanish]
- MOORE S.J., LENGLET A. & HILL N. 2007: Plant-based insect repellents. In Debboun M., Frances S.P. & Strickman D. (eds): Insect Repellents. CRC Press, Boca Raton, pp. 275-303
- NERIO L.S., OLIVERO-VERBEL J. & STASHENKO E. 2010: Repellent activity of essential oils: A review. - Bioresour. Technol. 101: 372-378
Go to original source...
- OMOLO M.O., OKINYO D., NDIEGE I.O., LWANDE W. & HASSANALI A. 2004: Repellency of essential oils of some Kenyan plants against Anopheles gambiae. - Phytochemistry 65: 2797-2802
Go to original source...
- PERRY R.H. & GREEN D. 1984: Perry's Chemical Handbook. Physical and Chemical data. McGraw-Hill, New York, 2640 pp
- PETRAKIS P.V., ROUSSIS V., PAPADIMITRIOU D., VAGIAS C. & TSITSIMPIKOU C. 2005: The effect of terpenoid extracts from 15 pine species on the feeding behavioral sequence of the late instars of the pine processionary caterpillar Thaumetopoea pityocampa. - Behav. Process. 69: 303-322
Go to original source...
- PICOLLO M.I., TOLOZA A.C., MOUGABURE CUETO G., ZYGADLO J. & ZERBA E. 2008: Anticholinesterase and pediculicidal activities of monoterpenoids. - Fitoterapia 79: 271-278
Go to original source...
- PHILLIPS A.K. & APPEL A.G. 2010: Fumigant toxicity of essential oils to the German cockroach (Dictyoptera: Blattellidae). - J. Econ. Entomol. 103: 781-90
Go to original source...
- PINCHIN R., DE OLIVEIRA FILHO A.M. & PEREIRA A.C.B. 1980: The flushing-out activity of pyrethrum and synthetic pyrethroids on Pastrongilus megistus, a vector of Chagas's disease. - Trans. R. Soc. Trop. Med. Hyg. 75: 691-694
Go to original source...
- RAJENDRAN S. & SRIRANJINI V. 2008: Plant products as fumigants for stored-product insect control. - J. Stored Prod. Res. 44: 126-135
Go to original source...
- RICE P.J. & COATS J.R. 1994a: Insecticidal properties of several monoterpenoids to the house fly (Diptera: Muscidae), red flour beetle (Coleoptera: Tenebrionidae), and southern corn rootworm (Coleoptera: Chrysomelidae). - J. Econ. Entomol. 87: 1172-1179
Go to original source...
- RICE P.J. & COATS J.R. 1994b: Insecticidal properties of monoterpenoid derivatives to the house fly (Diptera: Muscidae) and red flour beetle (Coleoptera: Tenebrionidae). - Pestic. Sci. 41: 195-202
Go to original source...
- SFARA V., ZERBA E.N. & ALZOGARAY R.A. 2009: Fumigant insecticidal activity and repellent effect of five essential oils and seven monoterpenes on first instar nymphs of Rhodnius prolixus. - J. Med. Entomol. 46: 511-515
Go to original source...
- SFARA V., MOUGABURE CUETO G.A., ZERBA E.N. & ALZOGARAY R.A. 2011: Adaptation of the repellency response to DEET in Rhodnius prolixus. - J. Insect Physiol. 57: 1431-1436
Go to original source...
- TARELLI G., ZERBA E.N. & ALZOGARAY R.A. 2009: Toxicity to vapor exposure and topical application of essential oils and monoterpenes on Musca domestica L. (Diptera: Muscidae). - J. Econ. Entomol. 102: 1383-1388
Go to original source...
- THRONE J.E., WEAVER D.K, CHEW V. & BAKER J.E. 1995: Probit analysis of correlated data: multiple observations over time at one concentration. - J. Econ. Entomol. 88: 1510-1512
Go to original source...
- TISSERAND R. & BALACS T. 1995: Essential Oil Safety. Livingstone, London, 279 pp
- TOLOZA A.C., ZYGADLO J., CUETO G.M., BIURRUN F., ZERBA E. & PICOLLO M.I. 2006: Fumigant and repellent properties of essential oils and component compounds against permethrinresistant Pediculus humanus capitis (Anoplura: Pediculidae) from Argentina. - J. Med. Entomol. 43: 889-895
Go to original source...
- TOMLIN C.D.S. 1997: The Pesticide Manual. 11th ed. BCPC, Farnham, 1608 pp
- WONG K.K.Y., SIGNAL F.A., CAMPION S.H. & MOTION R.L. 2005: Citronella as an insect repellent in food packaging. - J. Agric. Food Chem. 53: 4633-4636
Go to original source...
- ZAHRAN H.E.D.M. & ABDELGALEIL S.A.M. 2011: Insecticidal and inhibitory properties of monoterpenes on Culex pipiens L. (Diptera: Culicidae). - J. Asia-Pacific Entomol. 14: 46-51
Go to original source...
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