Eur. J. Entomol. 110 (2): 205-213, 2013 | 10.14411/eje.2013.031

Genetic analysis of quantitative trait loci for cocoon and silk production quantity in Bombyx mori (Lepidoptera: Bombycidae)

Bing LI1, Xiuye WANG3, Chengxiang HOU1,2, Anying XU2, Muwang LI*,1,2
1 Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu, China
2 Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu, China
3 Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China

Silk production quantity is the most economically important characteristic of the domesticated silkworm moth, Bombyx mori. It is controlled by multiple loci. The cocoon and silk production quantity of silkworm strains Jingsong and Lan10 have significantly diverged. A backcross population (BC1) was bred using Jingsong and Lan10 as parents to identify quantitative trait loci (QTLs) for silk quality. In this research, a genetic linkage map of the silkworm was constructed using the BC1 mapping population. The map contained 85 sequence-tagged site markers, 80 simple sequence repeat markers, and 16 single nucleotide polymorphisms. A linkage map was constructed from the data, which consisted of 181 markers distributed over 28 expected linkage groups and spans 2147.1 cM in total length. Fourteen QTLs were detected for cocoon filament length, whole cocoon weight, pupae weight, filament weight, and cocoon shell weight. The 14 QTLs were distributed in 5 linkage groups (linkage groups 1, 14, 18, 23 and 25) based on the constructed linkage map. In addition, five QTLs, which had the highest logarithm (base 10) of odds (LOD) values, were located on the first chromosome, three of which located at the same region in linkage group 1. These results represent an important foundation for the map-based cloning of QTLs and marker-assisted selection for improving the silk quality of economically important silkworm strains.

Keywords: Lepidoptera, Bombycidae, Bombyx mori, cocoon and silk production quantity, genetic linkage map, QTL mapping

Received: August 17, 2012; Accepted: November 28, 2012; Published: April 11, 2013

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