Eur. J. Entomol. 108 (4): 711-715, 2011 | 10.14411/eje.2011.091

Contribution to the biochemical characterization of the silk and structure characterization of the cocoons of the horse chestnut leaf miner Cameraria ohridella (Lepidoptera: Gracillariidae)

Veronika HNĚVSOVÁ1, Dalibor KODRÍK*,2,3, František WEYDA2,3
1 Gymnasium, Jírovcova 8, 371 61 České Budějovice, Czech Republic
2 Institute of Entomology, Biology Centre, Academy of Sciences and
3 Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic

This study of the proteins in the silk of the summer and winter cocoons of the horse chestnut leaf miner Cameraria ohridella revealed they can inhibit protease activity. The inhibitory activity of the summer silk was higher against both the fungal proteinase K and bacterial subtilisin than that of the winter silk. Also, the winter silk was more effective in inhibiting proteinase K than subtilisin. Further, it was demonstrated that some of the silk proteins are glycosylated probably by mannose carbohydrates. An electron microscopy study of the cocoons revealed the presence of silk fibres with different diameters in some pupal chambers.

Keywords: Silk, cocoon, pupal chamber, tissue of leaf mine, Cameraria ohridella, Gracillariidae, protease inhibitor, glycoprotein, electron microscopy

Received: April 28, 2011; Accepted: July 26, 2011; Published: October 3, 2011

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