Eur. J. Entomol. 108 (4): 553-559, 2011 | 10.14411/eje.2011.071

Molecular phylogenetic analysis of a scale insect (Drosicha mangiferae; Hemiptera: Monophlebidae) infesting mango orchards in Pakistan

Muhammad ASHFAQ1, Jehan ARA1, Ali Raza NOOR1, Paul D.N. HEBERT2, Shahid MANSOOR1
1 National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, 38000 Faisalabad, Pakistan; e-mails: muhammadashfaq@hotmail.com; ashfaqm@nibge.org
2 Biodiversity Institute of Ontario, University of Guelph, Canada; e-mail: phebert@uoguelph.ca

Mango orchards in Pakistan are attacked by the scale insect, Drosicha mangiferae (Hemiptera: Monophlebidae), commonly called the "mango mealybug". This insect is univoltine, active from December through May and targets multiple host plants. We used DNA nucleotide sequences to characterize and determine the phylogenetic status of D. mangiferae. Mango mealybugs were collected from several tree species from different localities and patterns of phylogenetic and genetic diversity were examined at both nuclear (18S, ITS1) and mitochondrial (COI) genes. Phylogenetic analysis confirms that the mango mealybug belongs to the family Monophlebidae. Minor genetic differences in both the ITS1 and the COI barcode region were noted among D. mangiferae collected from different geographic localities. These genetic differences revealed the existence of two genotypes of D. mangiferae that are region specific but not host-specific.

Keywords: Hemiptera, Monophlebidae, mango mealybug, Drosicha mangiferae, rDNA, COI, ITS1

Received: March 4, 2011; Accepted: May 31, 2011; Published: October 3, 2011

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