Eur. J. Entomol. 108 (2): 261-266, 2011 | 10.14411/eje.2011.035

Challenges of microsatellite development in Lepidoptera: Euphydryas aurinia (Nymphalidae) as a case study

Melthide SINAMA*,1, Vincent DUBUT*,1, Caroline COSTEDOAT1, André GILLES1, Marius JUNKER2, Thibaut MALAUSA3, Jean-François MARTIN4, Gabriel NÈVE1, Nicolas PECH1, Thomas SCHMITT2, Marie ZIMMERMANN5, Emese MEGLÉCZ1
1 Aix-Marseille Université, CNRS, IRD, UMR 6116 - IMEP, Equipe Evolution Génome Environnement, Case 36, 3 place Victor Hugo, 13331 Marseille Cedex 3, France; e-mails: melthide.sinama@gmail.com, vincent.dubut@univ-provence.fr, caroline.costedoat@univ-provence.fr, andre.gilles@univ-provence.fr, gabriel.neve@univ-provence.fr, nicolas.pech@univ-provence.fr, emese.meglecz@univ-provence.fr
2 Trier University, Department of Biogeography, 54286 Trier, Germany; e-mails: marius.junker@gmx.de, thsh@uni-trier.de
3 INRA, UMR 1301 - IBSV (INRA / UNSA / CNRS), Equipe Biologie des Populations en Interaction, 400 route des Chappes, BP 167, 06903 Sophia-Antipolis Cedex, France; e-mail: thibaut.malausa@sophia.inra.fr
4 Montpellier SupAgro, UMR CBGP (INRA / IRD / CIRAD / Montpellier SupAgro), Campus International de Baillarguet, CS 30016, 34988 Montferrier-sur-Lez Cedex, France; e-mail: martinjf@supagro.inra.fr
5 Université de Tours, CNRS, UMR 6035 - IRBI, Avenue Monge, Parc Grandmont, 37200 Tours, France; e-mail: marie.zimmermann@univ-tours.fr

Currently it remains difficult to obtain robust microsatellite markers for Lepidoptera. In an attempt to overcome the problems associated with developing microsatellite markers for this insect order we combined (i) biotin-enrichment protocol, (ii) next generation pyrosequencing (through 454 GS-FLX Titanium technology) and (iii) the use of individuals collected from eight geographically distant European populations representing three subspecies of Euphydryas aurinia. Out of 96 stringently designed primer pairs, 12 polymorphic microsatellite loci amplified without obvious evidence of null alleles in eight individuals from different subspecies. Between five and seven of these loci showed full within population applicability and three revealed to be robust and transferable between populations and sub-species, providing a first step towards the development of a valuable and robust tool for studying conservation issues and evolution in E. aurinia populations. Nevertheless, as in most studies dealing with Lepidoptera microsatellites, null alleles were detected in most of the developed markers. Our results emphasize the need for further research in order to better understand the complex evolution and organization of Lepidopteran genomes.

Keywords: Lepidoptera, microsatellite, null allele, 454 sequencing, QDD, Euphydryas aurinia

Received: August 9, 2010; Accepted: September 20, 2010; Published: April 5, 2011

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