Eur. J. Entomol. 108 (1): 161-163, 2011 | 10.14411/eje.2011.019

Highly polymorphic di- and trinucleotide microsatellite markers for the grapevine yellows disease vector Hyalesthes obsoletus (Auchenorrhyncha: Cixiidae)

Miriam IMO1, Julia LÜNEBURG1, Thomas HANKELN2, Alfred SEITZ*,1, Jes JOHANNESEN1
1 Department of Ecology, Zoological Institute, University of Mainz, J.-J.-Becher-Weg 13, D-55128 Mainz, Germany; e-mail: imo@uni-mainz.de
2 Institute of Molecular Genetics, University of Mainz, J.-J.-Becher-Weg 30a, D-55128 Mainz, Germany

Seven polymorphic microsatellite loci were developed for the planthopper Hyalesthes obsoletus, vector of stolbur 16SrXII-A phytoplasma. The loci have di- or trinucleotide repeat motifs and are highly variable with 10 to 22 alleles per locus. Observed heterozygosity ranged from 0.278 to 0.950 for the 78 individuals genotyped. One locus is sex-linked. No linkage between loci was found. All loci amplified consistently among phylogeographic as well as host-plant related samples and proved highly informative for population genetic studies.

Keywords: Cixiidae, Hyalesthes obsoletus, Bois noir, microsatellite, PCR

Received: June 2, 2010; Accepted: July 28, 2010; Published: January 3, 2011

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References

  1. AMID C., BAHR A., MUJICA A., SAMPSON N., BIKAR S.E., WINTERPACHT A., ZABEL B., HANKELN T. & SCHMIDT E.R. 2001: Comparative genomic sequencing reveals a strikingly similar architecture of a conserved syntenic region on human chromosome 11p15.3 (including gene ST5) and mouse chromosome 7. Cytogen. Cell Genet. 93: 284-290 Go to original source...
  2. JOHANNESEN J., LUX B., MICHEL K., SEITZ A. & MAIXNER M. 2008: Invasion biology and host-specificity of the grapevine yellows disease vector Hyalesthes obsoletus in Europe. Entomol. Exp. Appl. 126: 217-227 Go to original source...
  3. KOFLER R., SCHLOTTERER C. & LELLEY T. 2007: SciRoKo: a new tool for whole genome microsatellite search and investigation. Bioinformatics 23: 1683-1685 Go to original source...
  4. LANGER M. & MAIXNER M. 2004: Molecular characterisation of grapevine yellows associated phytoplasmas of the stolburgroup based on RFLP-analysis of non-ribosomal DNA. Vitis 43: 191-199
  5. MAIXNER M., AHRENS U. & SEEMULLER E. 1995: Detection of the German grapevine yellows (Vergilbungskrankheit) MLO in grapevine, alternative hosts and a vector by a specific PCR procedure. Eur. J. Plant Pathol. 101: 241-250 Go to original source...
  6. MAIXNER M., JOHANNESEN J. & SEITZ A. 2009: Aspects of the interaction of stolbur phytoplasma, vectors and host plants in the epidemic systems of Bois Noir. Progres Agricole et Viticole, Hors Serie - Extended abstracts, 16th Meeting of ICVG, Dijon, France, 31 August - 4 September, pp. 141-142
  7. PRITCHARD J.K., STEPHENS M. & DONNELLY P. 2000: Inference of population structure using multilocus genotype data. Genetics 155: 945-959
  8. RAYMOND M. & ROUSSET F. 1995: GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J. Hered. 86: 248-249
  9. RICE W.R. 1989: Analyzing tables of statistical tests. Evolution 43: 223-225 Go to original source...
  10. SCHUELKE M. 2000: An economic method for the fluorescent labelling of PCR fragments. Nature Biotechnol. 18: 233-234 Go to original source...
  11. VAN OOSTERHOUT C.V., HUTCHINSON W.F., WILLS D.P.M. & SHIPLEY P. 2004: Micro-Checker: software for identifying and correcting genotying errors in microsatellite data. Mol. Ecol. 4: 535-538 Go to original source...
  12. WEINTRAUB P.G. & BEANLAND L. 2006: Insect vectors of phytoplasmas. Annu. Rev. Entomol. 51: 91-111 Go to original source...
  13. WEIR B.S. & COCKERHAM C.C. 1984: Estimating F-Statistics for the analysis of population structure. Evolution 38: 1358-1370 Go to original source...