Eur. J. Entomol. 107 (4): 687-690, 2010 | 10.14411/eje.2010.078

An improved method for the culture of wing discs of the wingless bagworm moth, Eumeta variegata (Lepidoptera: Psychidae)

Shuhei NIITSU1, Saori LOBBIA2
1 Department of Life Science, International Christian University, Osawa 3-10-2, Mitaka, Tokyo 181-8585, Japan; e-mail: shu-30@aioros.ocn.ne.jp
2 Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Bioscience Building 501, Kashiwanoha, Kashiwa, Chiba 277-8562, Japan

Adults of the wingless bagworm moth, Eumeta variegata, show remarkable sexual dimorphism. Final-instar larvae of the male have invaginated wing discs, whereas those of the female are rudimentary. To determine the best method for culturing the wing discs of E. variegata, which in both sexes are attached to the larval integument, two methods of culturing the larval wing discs are compared. Initially, a stationary culture was used. In these cultures necrotic cells and degeneration of wing discs of males were sporadically observed. By contrast, many small vacuoles were observed in the female wing rudiment under these conditions. In order to overcome some of the problems associated with stationary culture, rotating culture was used and resulted in the wing discs of males and females remaining in good condition. A histological analysis revealed that the wing disc morphology was normal when they were cultured in this way. These results indicate that rotating the culture medium is the better procedure for studying the action of hormones on the differentiation and metamorphosis of reduced wing rudiments in E. variegata.

Keywords: Lepidoptera, Psychidae, Eumeta variegata, bagworm moth, wing disc, tissue culture, differentiation

Received: January 29, 2010; Accepted: April 27, 2010; Published: October 20, 2010

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