Eur. J. Entomol. 107 (4): 673-675, 2010 | 10.14411/eje.2010.074

Characterization of polymorphic microsatellite loci in the neotropical plant-ant Allomerus decemarticulatus (Formicidae: Myrmicinae) and multiplexing with other microsatellites from the ant subfamily Myrmicinae

Pierre-Jean G. MALÉ1,2, Anne LOISEAU3, Arnaud ESTOUP3, Angélique QUILICHINI4,5, Jérôme ORIVEL1,2,4
1 Université de Toulouse, Laboratoire Evolution & Diversité Biologique (EDB), UMR 5174, 118 route de Narbonne, 31062 Toulouse cedex 9, France; e-mails: pjmale@cict.fr, orivel@cict.fr
2 CNRS, EDB (Laboratoire Evolution et Diversité Biologique), F-31062 Toulouse, France
3 INRA, UMR CBGP (INRA / IRD / Cirad / Montpellier SupAgro), Campus international de Baillarguet, CS 30016, F-34988 Montferrier-sur-Lez cedex, France; e-mails: loiseau@supagro.inra.fr, estoup@supagro.inra.fr
4 CNRS, UMR Ecologie des Forêts de Guyane, Campus Agronomique, F-97379 Kourou cedex, France; e-mail: Angelique.Quilichini@ecofog.gf
5 Jardin Botanique Henri Gaussen, 39 allées Jules Guesde, F-31062 Toulouse, France

Five polymorphic microsatellite loci of the arboreal ant Allomerus decemarticulatus (Myrmicinae) were isolated and characterized. The amplification and polymorphism of seven additional microsatellite loci, previously developed for the ant species A. octoarticulatus and Wasmannia auropunctata, were also tested and the amplification conditions necessary for genotyping the complete set of 12 multiplexed markers in A. decemarticulatus determined. The number of alleles per locus ranged from three to 15 and observed heterozygosity varied from 0.09 to 0.95. Cross-species amplification of these loci was also successfully achieved in additional species of the same ant subfamily, Myrmicinae. This set of microsatellite markers will be used in studies on the mating system and population genetic structure of Myrmicinae in general and A. decemarticulatus in particular.

Keywords: Hymenoptera, Formicidae, Myrmicinae, Allomerus, ant-plant mutualism, microsatellites, plant-ant

Received: March 30, 2010; Accepted: May 10, 2010; Published: October 20, 2010

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