Eur. J. Entomol. 107 (4): 509-520, 2010 | DOI: 10.14411/eje.2010.058

Analysis of the lipids mobilized by adipokinetic hormones in the firebug Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae)

Iva BÁRTŮ1,2, Aleš TOMČALA1,3, Radomír SOCHA1, Petr ŠIMEK1, Dalibor KODRÍK*,1,2
1 Institute of Entomology, Biology Centre, Academy of Sciences, Branišovská 31, 370 05 České Budějovice, Czech Republic; e-mail: kodrik@entu.cas.cz
2 Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic
3 Institute of Organic Chemistry and Biochemistry, Academy of Sciences, Flemingovo náměstí 2, 166 10 Praha 6, Czech Republic

Effects of two adipokinetic hormones (Pyrap-AKH and Peram-CAH-II) on the presence of diacylglycerol (DG) molecular species and their fatty acid (FA) constituents in the haemolymph of the firebug Pyrrhocoris apterus were investigated using liquid chromatography (HPLC) and electrospray ionization mass spectrometry (ESI-MS). The results show that DGs with characteristic FAs are preferentially mobilized from the fat body (FB) by the action of both the AKHs produced by P. apterus. Both the macropterous and brachypterous morphs have similar DG and FA profiles. A difference in the action of the Pyrap-AKH and the Peram-CAH-II, however, results in distinct differences in the distribution of FAs in the macropterous morph. It seems that C16 to a slight extent and unsaturated C18 FAs mainly play a dominant role in the AKH based action, in particular linoleic acid (18:2), which represents 50-60% of the total DG mobilized. The metabolically active C16 and C18 FAs are preferentially absorbed from the linden seeds and accumulated in the FB. The relationships between AKH action and FA distribution in DGs in P. apterus, compared to other insect species are summarized and discussed in detail.

Keywords: Heteroptera, Pyrrhocoris apterus, AKH, lipid, triacylglycerol, diacylglycerol, fatty acid, haemolymph, fat body, linden seed, HPLC, ESI-MS, mass spectrometry

Received: March 24, 2010; Accepted: May 12, 2010; Published: October 20, 2010

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