Eur. J. Entomol. 107 (3): 317-323, 2010 | 10.14411/eje.2010.040

Kinetic activity of the sex chromosomes of Mormidea paupercula (Heteroptera: Pentatomidae)

Pablo Javier REBAGLIATI1, Liliana María MOLA1,2
1 Laboratorio de Citogenética y Evolución, Departamento de Ecología Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes y Costanera Norte, 1428 Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, Argentina; e-mails: gchu@ege.fcen.uba.ar, limola@ege.fcen.uba.ar
2 Investigador del Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET)

In Mormidea paupercula (n = 6 + XY in males), the presence of a CMA3-bright band in the telomeric regions on both sex chromosomes allowed the analysis of the kinetic activity of the sex univalents and XY pseudobivalent at the first and second meiotic divisions, respectively. The separation of the sister chromatids of the sex chromosomes occurs from a pair of telomeric regions (with or without a band), with opposite telomeric regions remaining associated with each other at meiosis I; the behaviour of both sex chromosomes differs, on the X chromosome both telomeric regions are similarly active, while on the Y chromosome the telomeric region without a band is more frequently active. At the second division, the most frequent associations in the pseudobivalent occur between the telomeric regions of both sex chromosomes with bands or without bands. Therefore, in both meiotic divisions, the same telomeric region on the sex chromosomes could lead the migration, in contrast to that observed usually in autosomal bivalents. These results provide evidence that the sex chromosomes of Heteroptera show more than one pattern of attachment to the spindle.

Keywords: Heteroptera, Pentatomidae, sex chromosomes, kinetic activity, holokinetic chromosomes, meiosis, Mormidea

Received: October 30, 2009; Accepted: March 26, 2010; Published: July 20, 2010

Download citation

References

  1. BATTAGLIA E. & BOYES J.W. 1955: Post-reductional meiosis: its mechanism and causes. Caryologia 8: 86-134 Go to original source...
  2. BUCK R.C. 1967: Mitosis and meiosis in Rhodnius prolixus. The fine structure of the spindle and diffuse kinetochore. J. Ultrastruct. Res. 18: 489-501 Go to original source...
  3. CAMACHO J.P.M., BELDA J. & CABRERO J. 1985: Meiotic behaviour of the holocentric chromosomes of Nezara viridula (Insecta, Heteroptera) analysed by C-banding and silver impregnation. Can. J. Genet. Cytol . 27: 490-497 Go to original source...
  4. CATTANI M.V., GREIZERSTEIN E.J. & PAPESCHI A.G. 2004: Male meiotic behavior and nucleolus organizer regions in Camptischium clavipes (Fabr.) (Coreidae, Heteroptera) analyzed by fluorescent banding and in situ hybridization. Caryologia 57: 267-273 Go to original source...
  5. COMINGS D.E. & OKADA T.A. 1972: Holocentric chromosomes in Oncopeltus: kinetochore plates are present in mitosis but absent in meiosis. Chromosoma 37: 177-192 Go to original source...
  6. CRINITI A., SIMONAZZI G., CASSANELLI S., FERRARI M., BIZZARO D. & MANICARDI G.C. 2009: Distribution of heterochromatin and rDNA on the holocentric chromosomes of the aphids Dysaphis plantaginea and Melanaphis pyraria (Hemiptera: Aphididae). Eur. J. Entomol. 106: 153-157 Go to original source...
  7. GONZALEZ-GARCIA J.M., BENAVENTE R. & RUFAS J.S. 1996a: Cytochemical and immunochemical characterization kinetochores in the holocentric chromosomes of Graphosoma italicum. Eur. J. Cell Biol . 70: 352-360
  8. GONZALEZ-GARCIA J.M., ANTONIO C., SUJA J.A. & RUFAS J.S. 1996b: Meiosis in holocentric chromosomes: kinetic activity is randomly restricted to the chromatid ends of sex univalents in Graphosoma italicum (Heteroptera). Chromosome Res. 4: 124-132 Go to original source...
  9. HEIZER P. 1950: The chromosome cytology of two species of the Pacific genus Oechalia (Pentatomidae, Hemiptera - Heteroptera) Oechalia patruelis Stal and Oechalia pacifica Stal. J. Morphol. 87: 179-226 Go to original source...
  10. ITUARTE S. & PAPESCHI A.G. 2004: Achiasmatic male meiosis in Tenagobia (Fuscagobia) fuscata (Stal) (Heteroptera, Corixoidea, Micronectidae). Genetica 122: 199-206 Go to original source...
  11. MANDRIOLI M., BIZZARO D., GIUSI M., MANICARDI G.C. & BIANCHI U. 1999: The role of rDNA genes in X chromosome association in the aphid Acyrthosiphon pisum. Genome 42: 381-386 Go to original source...
  12. MOLA L.M. & PAPESCHI A.G. 2006: Holokinetic chromosomes at a glance. J. Basic Appl. Genet. 17: 17-33.
  13. NOKKALA S. 1985: Restriction of kinetic activity of holokinetic chromosomes in meiotic cells and its structural basis. Hereditas 102: 85-88 Go to original source...
  14. NOKKALA S. & GROZEVA S.M. 2000: Achiasmatic male meiosis in Myrmnedonia coleoptrata (Fn.) (Heteroptera: Microphysidae). Caryologia 53: 5-8 Go to original source...
  15. NOKKALA S. & NOKKALA C. 1984: Achiasmatic male meiosis in the Heteropteran genus Nabis (Nabidae, Hemiptera). Hereditas 101: 31-35 Go to original source...
  16. PAGE J., DE LA FUENTE R., GOMEZ R., CALVENTE A., VIERA A., PARRA M.T., SANTOS J.L., BERRIOS S., FERNANDEZ-DONOSO R., SUJA J.A. & RUFAS J. 2006: Sex chromosomes, synapsis, and cohesins: a complex affair. Chromosoma 115: 250-259 Go to original source...
  17. PEREZ R., PANZERA F., PAGE J., SUJA J.A. & RUFAS J.S. 1997: Meiotic behaviour of holocentric chromosomes: orientation and segregation of autosomes in Triatoma infestans (Heteroptera). Chromosome Res. 5: 47-56 Go to original source...
  18. PEREZ R., RUFAS J.S., SUJA J.A., PAGE J. & PANZERA F. 2000: Meiosis in holocentric chromosomes: orientation and segregation of an autosome and sex chromosomes in Triatoma infestans (Heteroptera). Chromosome Res. 8: 17-25 Go to original source...
  19. REBAGLIATI P.J., PAPESCHI A.G. & MOLA L.M. 2003: Meiosis and fluorescent banding in Edessa meditabunda and Edessa rufomarginata (Heteroptera: Pentatomidae: Edessinae). Eur. J. Entomol. 100: 11-18 Go to original source...
  20. ROY V., MONTI-DEDIEU L., CHAMINADE N., SILJAK-YAKOVLEV S., AULARD S., LEMEUNIER F. & MONTCHAMP-MOREAU C. 2005: Evolution of the chromosomal location of rDNA genes in two Drosophila species subgroups: ananassae and melanogaster. Heredity 94: 388-395 Go to original source...
  21. SCHRADER F. 1940: The formation of tetrads and meiotic mitoses in the male of Rhytidolomia senilis Say (Hemiptera, Heteroptera). J. Morphol. 67: 123-141 Go to original source...
  22. SCHRADER F. 1945a: Regular occurrence of heteroploidy in a group of Pentatomidae (Hemiptera). Biol. Bull. 88: 63-70 Go to original source...
  23. SCHRADER F. 1945b: The cytology of regular heteroploidy in the genus Loxa (Pentatomidae - Hemiptera). J. Morphol. 76: 157-177 Go to original source...
  24. SCHRADER F. & HUGHES-SCHRADER S. 1956: Polyploidy and fragmentation in the chromosomal evolution of various species of Thyanta (Hemiptera). Chromosoma 7: 469-497 Go to original source...
  25. SCHRADER F. & HUGHES-SCHRADER S. 1958: Chromatid autonomy in Banasa (Hemiptera: Pentatomidae). Chromosoma 9: 193-215 Go to original source...
  26. SRIVASTAVA M.D.L. 1957: Compound sex chromosome mechanism and regular occurring meiotic aberrations in the spermatogenesis of Macropygium reticulare (Pentatomidae - Heteroptera). Cellule 58: 252-274
  27. UESHIMA N. 1979: Hemiptera II: Heteroptera. In John B. (ed.): Animal Cytogenetics. Vol. 3, Insecta 6. Gebrueder Borntraeger Berlin, pp. 1-113
  28. VIERA A., PAGE J. & RUFAS J.S. 2009a: Inverted meiosis: The true bugs as a model to study. Genome Dyn. 5: 137-156 Go to original source...
  29. VIERA A., SANTOS J.L., PARRA M.T., CALVENTE A., GOMEZ R., DE LA FUENTE R., SUJA J.A., PAGE J. & RUFAS J.S. 2009b: Cohesin axis maturation and presence of RAD51 during first meiotic prophase in a true bug. Chromosoma 118: 1-15 Go to original source...
  30. WILSON E. 1906: Studies on chromosomes III. The sexual differences of the chromosome-groups in Hemiptera, with some considerations on the determination and inheritance of sex. J. Exp. Zool. 3: 1-40 Go to original source...
  31. WILSON E. 1909: Studies on chromosomes IV. The accessory chromosome in Syromastes and Pyrrhocoris, with comparative review of the types of sexual difference of the chromosome groups. J. Exp. Zool. 6: 69-99 Go to original source...
  32. WILSON E. 1911: Studies on chromosomes VII. A review of the chromosomes of Nezara with some more general considerations. J. Morphol. 22: 71-110 Go to original source...
  33. WOLFE S.L. & JOHN B. 1965: The organization and ultrastructure of male meiotic chromosomes in Oncopeltus fasciatus. Chromosoma 17: 85-103 Go to original source...