Eur. J. Entomol. 107 (2): 267-269, 2010 | 10.14411/eje.2010.034

Isolation and characterization of six microsatellite loci in the larch budmoth Zeiraphera diniana (Lepidoptera: Tortricidae)

Sophie DELAMAIRE1, G. Danny ESSELINK2, Leila SAMIEI2,3, Claudine COURTIN1, Emmanuelle MAGNOUX1, Jérôme ROUSSELET1, M.J.M. SMULDERS2
1 INRA UR0633 Zoologie Forestière Orléans, 2163 Avenue de la Pomme de Pin, 45075 Orléans, France; e-mail: sophiedelamaire@yahoo.fr
2 Plant Research International, Wageningen UR Plant Breeding, P.O. Box 16, NL-6700 AA Wageningen
3 Dept. of Horticulture, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

Six microsatellite markers were developed for the larch budmoth Zeiraphera diniana Guénée 1845, using two enrichment protocols. The number of alleles ranged from 3 to 15 per locus and observed heterozygosities ranged from 0.09 to 0.98 for the 69 individuals genotyped. Using these markers significant genetic differentiation between one population from Poland and samples from Alpine populations in France and Switzerland (overall FST = 0.0298) was detected. However, the two Alpine samples did not differ significantly. These microsatellite markers are valuable tools for studying the population genetics of Zeiraphera diniana.

Keywords: Lepidoptera, Tortricidae, Zeiraphera diniana, larch budmoth, microsatellite markers, outbreak

Received: December 2, 2009; Accepted: February 1, 2010; Published: April 15, 2010

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