Eur. J. Entomol. 107 (2): 147-156, 2010 | DOI: 10.14411/eje.2010.020

"DNA barcoding" is of limited value for identifying adelgids (Hemiptera: Adelgidae) but supports traditional morphological taxonomy

Martina ®UROVCOVÁ1,2, Jan HAVELKA1, Petr STARÝ1, Pavlína VĚCHTOVÁ2, Daniela CHUNDELOVÁ2, Andrea JARO©OVÁ2, Lucie KUČEROVÁ1,2
1 Biology Centre ASCR, Institute of Entomology, Braniąovská 31, CZ-37005 České Budějovice, Czech Republic; e-mail: martina@entu.cas.cz
2 Faculty of Science, University of South Bohemia, Braniąovská 31, CZ-370 05 České Budějovice, Czech Republic

The sequence diversity in the mitochondrial cytochrome-c oxidase I (COI) gene was evaluated as a tool for resolving differences among species of European adelgids collected from several localities across the Czech Republic. Members of 7 genera and 16 species were examined, and as outgroups, two species of Phylloxeridae were used. Sequence divergences within species were on average less than 0.15%, whereas divergences between species ranged from 0.0 to 4.12% for congeneric and to 13.24% for intergeneric comparisons. It is concluded that DNA barcoding of Adelgidae is a powerful tool for identifying genera, but at the species level it works only in those cases where there are no species complexes. Nevertheless, it can be used as a complement to traditional, morphological taxonomy.

Keywords: Adelgidae, aphids, species identification, systematics, DNA barcoding

Received: October 1, 2009; Accepted: January 4, 2010; Published: April 15, 2010

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