Eur. J. Entomol. 107 (2): 137-146, 2010 | DOI: 10.14411/eje.2010.019

Genetic diversity, population structure and taxonomy of Calopteryx splendens (Odonata: Calopterygidae): An AFLP analysis

Saber SADEGHI1,2, Tina KYNDT3, Henri J. DUMONT*,1
1 Department of Biology, Gent University, Ledeganckstraat 35, B-9000 Gent, Belgium
2 Department of Biology, Shiraz University, Shiraz, Iran; e-mail: sabersadeghi@yahoo.com
3 Department of Molecular Biotechnology, Gent University, Coupure links 653, B-9000 Gent, Belgium; e-mail: tina.kyndt@ugent.be

Calopteryx splendens is a widely distributed palaearctic damselfly with a remarkably uniform morphology. Variation in the size and shape of the pigmented spot on the wing is the main diagnostic character used to discriminate subspecies across its huge geographic range. Here, AFLP analysis was used to assess the genetic structure and diversity of nine populations representing 3 putative subspecies and evaluate the pigment spot as a taxonomic marker. Genetic diversity was high, with the number of polymorphic loci per population ranging from 141 to 280 out of a total of 333 variable sites (42.3-84.1%) and Nei's gene diversity from 0.160 to 0.283 (overall 0.299). Overall population genetic differentiation (FST = 0.2766) suggests limited gene flow and adaptation to local environments. Restricted gene flow and genetic differentiation among populations are supported by significant FST estimates. High levels of gene flow (Nm >1) were only recorded among three Asian populations (Russia - Kazakhstan - Turkey). The patterns of genotypic diversity suggest that a given wing spot size and shape may arise from the hybridization of a limited number, possibly not more than four, ancestral gene pools in different ways and at different times. Clearly, the sample analyzed was not sufficient to capture all of the complex history of C. splendens, but sufficient to indicate the taxa ancilla, waterstoni, and orientalis possibly represent three of the four ancestral gene pools, and originated in western Asia. The origin of the fourth, xanthostoma, is the western Mediterranean.

Keywords: Odonata, Calopteryx, AFLP, genetic structure, diversity, gene flow

Received: September 7, 2009; Accepted: December 21, 2009; Published: April 15, 2010

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References

  1. ALBERT V., JONSSON B. & BERNATCHEZ L. 2006: Natural hybrids in Atlantic eels (Anguilla anguilla, A. rostrata): evidence for successful reproduction and fluctuating abundance in space and time. Mol. Ecol. 15: 1903-1191 Go to original source...
  2. ALLENDORF F.W. & LUIKART G. 2007: Conservation and the Genetics of Populations. Blackwell Scientific, Malden, MA, 664 pp
  3. ASKEW R.R. 2004: The Dragonflies of Europe. Midas Printing, China, 308 pp
  4. BENSCH S. & AKESSON M. 2005: Ten years of AFLP in ecology and evolution: why so few animals? Mol. Ecol. 14: 2899-2914 Go to original source...
  5. BODENHEIMER F.S. 1935: Animal Life in Palestine. Mayer, Jerusalem, 507 pp
  6. BRAKEFIEL P.M. & REITSMA N. 1991: Phenotypic plasticity, seasonal climate and the population biology of Bycyclus butterflies (Satyridae) in Malawi. Ecol. Entomol. 16: 291-303 Go to original source...
  7. CHAPUT-BARDY A., LEMAIRE C., PICARD D. & SECONDI J. 2008: In-stream and overland dispersal across a river network influences gene flow in a freshwater insect, Calopteryx splendens. Mol. Ecol. 17: 3496-3505 Go to original source...
  8. CLARK A.G. & LANIGAN C.M. 1993: Prospects for estimating nucleotide divergence with RAPDs. Mol. Biol. Evol. 10: 1096-1111
  9. CORBET P.S. 2004: Dragonflies, Behaviour and Ecology of Odonata. Rev. ed. Harley Books, Essex, 829 pp
  10. CORDOBA-AGUILAR A. 2002: Wing pigmentation in territorial male damselflies, Calopteryx haemorrhoidalis; a possible relation to sexual selection. Anim. Behav. 63: 759-766 Go to original source...
  11. DUMONT H.J. 1972: The taxonomic status of Calopteryx xanthostoma (Charpentier, 1825) (Zygoptera: Calopterigidae). Odonatologica 1: 21-29
  12. DUMONT H.J. 1975a: Endemic dragonflies of late Pleistocene age of the Hula lake area (North Israel), with notes on the Calopterygidae of the rivers Jordan (Israel, Jordan) and Litani (the Lebanon), and description of Urothemis edwardsi hulae subspec. nov. (Libellulidae). Odontologica 4: 1-9
  13. DUMONT H.J. 1975b: A note on some dragonflies from Afghanistan. Odonatologica 4: 243-248
  14. DUMONT H.J., DEMIRSOY A. & VERSCHUREN D. 1987: Breaking the Calopteryx-bottleneck: taxonomy and range of Calopteryx splendens waterstoni Schneider, 1984 and C. splendens tschaldirica Bartenef, 1909 (Zygoptera: Calopterygidae). Odonatologica 16: 239-247
  15. DUMONT H.J., HARITONOV A.Y. & BORISOV S.N. 1992: Larval morphology and range of three West Asiatic species of the genus Onychogomphus Selys, 1854 (Insecta: Odonata). Hydrobiologia 245: 169-177 Go to original source...
  16. DUMONT H.J., HEIDARI H. & ATAMURADOV K.I. 1997: Hybridisation in Calopteryx orientalis (Selys) East of the shores of the south Caspian lake (Zygoptera: Calopterygidae). Odonatologica 26: 205-213
  17. DUMONT H.J., VANFLETEREN J.R., DE JONCKHEERE J.F. & WEEKERS P.H.H. 2005: Phylogenetic relationships, divergence time estimation, and global biogeographic patterns of calopterygoid damselflies (Odonata, Zygoptera) inferred from ribosomal DNA sequences. Syst. Biol. 54: 347-362 Go to original source...
  18. EBER S. & BRANDL R. 1997: Genetic differentioation of the tephritid fly Urophora cardui in Europe as evidence for its biogeographical history. Mol. Ecol. 6: 651-660 Go to original source...
  19. EVANNO G., REGNAUT S. & GOUDET J. 2005: Detecting the number of clusters of individuals using the software STRUCTURE : a simulation study. Mol. Ecol. 14: 2611-2620 Go to original source...
  20. EXCOFFIER L., LAVAL G. & SCHNEIDER S. 2005: Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evol. Bioinform. 1: 47-50
  21. FALUSH D., STEPHENS M. & PRITCHARD J.K. 2003: Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics 164: 1567-1587
  22. FALUSH D., STEPHENS M. & PRITCHARD J.K. 2007: Inference of population structure using multilocus genotype data: dominant markers and null alleles. Mol. Ecol. Notes 7: 574-578 Go to original source...
  23. GRETHER G.F. 1996: Sexual selection and survival selection on wing coloration and body size in the rubyspot damselfly Hetaerina americana. Evolution 50: 1939-1948 Go to original source...
  24. GUPPY C.S. 1986: The adaptive significance of alpine melanism in the butterfly Parnassius phoebus F. (Lepidoptera: Papilionidae). Oecologia 70: 205-213 Go to original source...
  25. HEWITT G.M. 2001: Speciation, hybrid zones and phylogeography - or seeing genes in space and time. Mol. Ecol. 10: 537-549 Go to original source...
  26. HOOPER R.E., TSUBAKI Y. & SIVA-JOTHY M.T. 1999: Expression of a costly secondary sexual trait is correlated with age and condition in a damselfly with two male morphs. Physiol. Entomol. 24: 364-369 Go to original source...
  27. HOPEMAN M. & ABRAMSON Z.R. 2005: Sexual dimorphism in the dark-winged damselfly Calopteryx Maculata. Online: http://www.umich.edu/~umforum/Issue2/Issue2-p28.pdf
  28. HUGHES J.M., MATHER P.B., HILLYER M.J., CLEARY C. & PECKARSKY B. 2003: Genetic structure in a montane mayfly Baetis bicaudatus (Ephemeroptera: Baetidae), from the Rocky Mountains, Colorado. Freshwat. Biol. 48: 2149-2162 Go to original source...
  29. INNAN H., TERAUCHI R., KAHL G. & TAJIMA F. 1999: A method for estimating nucleotide diversity from AFLP data. Genetics 151: 1157-1164
  30. JACCARD P. 1908: Nouvelles recherches sur la distribution florale. Bull. Soc. Vaud. Sci. Nat. 44: 223-270
  31. JORDE L. 1980: The genetic structure of human populations: A review. In Mielke J. & Crawford M. (eds): Current Developments in Anthropological Genetics. Plenum, New York, pp. 135-208
  32. KELLY L.C., BILTON D.T. & RUNDLE S.D. 2002: Genetic population structure and dispersal in Atlantic Island caddisflies. Freshwat. Biol. 47: 1642-1650 Go to original source...
  33. KOLYER J.M. 1966: The effect of certain environmental factors and chemicals on the markings of Pieris rapae (Pieridae). J. Lepidopt. Soc. 20: 13-27
  34. KOSAREV A.N. & YABLONSKAYA E.A. 1994: The Caspian Sea. SPB Academic Publishing, The Hague, 260 pp
  35. KOSKIMAKI J., MARKUS K.I., RANTALA J., TASKINEN J., TYNKKYNEN K. & SUHONEN K. 2004: Immunocompetence and resource holding potential in the damselfly, Calopteryx virgo L. Behav. Ecol. 15: 169-173 Go to original source...
  36. LINDEBOOM M. 1998: Post-copulatory behaviour in Calopteryx females (Insecta, Odonata, Calopterygidae). Int. J. Odonatol. 1: 175-184 Go to original source...
  37. LOWE A.J., JOURDE B., BREYNE P., COLPAERT N., NAVARRO C., WILSON J. & CAVERS S. 2003: Fine-scale genetic structure and gene flow within Costa Rican populations of mahogany (Swietenia macrophylla). Heredity 90: 268-275 Go to original source...
  38. LYNCH M. & MILLIGAN B.G. 1994: Analysis of population genetic structure with RAPD markers. Mol. Ecol. 3: 91-99 Go to original source...
  39. MANTEL N. 1967: The detection of disease clustering and a generalized regression approach. Cancer Res. 27: 209-220
  40. MUELLER U.G. & WOLFENBARGER L.L. 1999: AFLP genotyping and fingerprinting. Trends Ecol. Evol. 14: 389-394 Go to original source...
  41. NEI M. 1973: Analysis of gene diversity in subdivided populations. Proc. Nat. Acad. Sci. USA 70: 3321-3323 Go to original source...
  42. PAPAZIAN M. 1995: Etude systematique et biogeographique de Calopteryx splendens (Harris, 1782) en Provence (Odonata, Zygoptera). Bull. Soc. Entomol. Fr. 100: 361-376
  43. PARKES K.A., AMOS W., MOORE N.W., HOFFMAN J.I. & MOORE J. 2009: Population stracture and speciation in the dragonfly Sympetrum strialatum/nigrescens (Odonata: Libellulidae): An analysis using AFLP markers. Eur. J. Entomol. 106: 179-184 Go to original source...
  44. PRITCHARD J.K. & ROSENBERG N.A. 1999: Use of unlinked genetic markers to detect population stratification in association studies. Am. J. Hum. Genet. 65: 220-228 Go to original source...
  45. PRITCHARD J., STEPHENS M. & DONNELLY P. 2000: Inference of population structure using multilocus genotype data. Genetics 155: 945-959
  46. ROSENBERG N.A. 2004: DISTRUCT, a program for the graphical display of population structure. Mol. Ecol. Notes 4: 137-138 Go to original source...
  47. ROSENBERG N.A., PRITCHARD J.K., WEBER J.L., CANN H.M., KIDD K.K., ZHIVOTOVSKY L.A. & FELDMAN M.W. 2002: Genetic structure of human populations. Science 298: 2381-2385 Go to original source...
  48. ROHLF F.J. 2004: NTSYSpc Numerical Taxonomy and Multivariate Analysis System. Version 2.11 User Guide. Exeter Software, Setauket, NewYork
  49. SALVATO A., BATTISTI A., CONCATO S., MASUITTI L., PATARNELLO T. & ZANE L. 2002: Genetic differentiation in the winter pine processionary moth (Thaumetopoea pityocampa-wilkinsoni complex), inferred by AFLP and mitochondrial DNA markers. Mol. Ecol. 11: 2435-2444 Go to original source...
  50. SCHNEIDER S., KUFFER J.M., ROESSLI D. & EXOFFIER L. 1996: Arlequin: A Software Package for Population Genetics. Version 3.1.1. Genetics, Biometry Lab, Department of Anthropology, University of Geneva
  51. SCHNEIDER S., ROESSLI D. & EXOFFIER L. 2000: Arlequin: A Software Package for Population Genetics. Biometry Lab, Department of Anthropology, University of Geneva
  52. SIVA-JOTTHY M. 2000: A mechanistic link between parasite resistance and expression of sexually selected trait in a damselfly. Proc. R. Soc. Lond. 267: 2523-2527 Go to original source...
  53. SLATKIN M. 1987: Gene flow and geographic structure of natural populations. Science 236: 787- 292 Go to original source...
  54. SLATKIN M. 1989: Detecting small amounts of gene flow from phylogenies of alleles. Genetics 121: 609-612
  55. SLATKIN M. 1991: Inbreeding coefficients and coalescence times. Genet. Res. 58: 167-175 Go to original source...
  56. SOKAL R.R. & MICHENER C.D. 1958: A statistical method for evaluating systematic relationships. Univ. Kansas Sci. Bull. 38: 1409-38
  57. SOKAL R.R. & ROHLF F.J. 1995: Biometry: The Principles and Practice of Statistics in Biological Research. 3rd. ed. W.H. Freeman, San Francisco
  58. SOKAL R.R. & WARTENBERG D.E. 1983: A test of spatial autocorrelation analysis using an isolation-by-distance model. Genetics: 105, 219-237
  59. STETTMER C. 1996: Colonisation and dispersal patterns of banded (Calopteryx splendens) and beautiful demoiselles (C. virgo) (Odonata: Calopterygidae) in south-east German streams. Eur. J. Entomol. 93: 579-593
  60. SVENSSON E.I., KRISTOFFERSEN L., OSKARSSON K. & BENSCH S. 2004: Molecular population divergence and sexual selection on morphology in the banded demoiselle (Calopteryx splendens). Heredity 93: 423-433 Go to original source...
  61. TORRES C., RIBAS G., XAMENA N., CREUS A. & MARCOS R. 1992: Genotoxicity of four herbicides in the Drosophila wing spot. Mutat. Res. 280: 291-295 Go to original source...
  62. TYNKKYNEN K., RANTALA M.J. & SUHONEN J. 2004: Interspecific aggression and character displacement in the damselfly Calopteryx splendens. J. Evol. Biol. 17: 259-267 Go to original source...
  63. URBANEK M., GOLDMAN D. & LONG J.C. 1996: The apportionment of dinucleotide repeat diversity in Native Americans and Europeans: A new approach to measuring gene identity reveals asymmetric patterns of divergence. Mol. Biol. Evol. 13: 943-953 Go to original source...
  64. VEKEMANS X. 2002: AFLP-SURV version 1.0. Distributed by the author. Laboratoire de Genetique et Ecologie Vegetale, Universite Libre de Bruxelles, Belgium, (http://www.ulb.ac. be/sciences/lagev/aflp-surv.html)
  65. VOS P., HOGERS R., BLEEKER M., REIJANS M., VAN DE LEE T., HORNES M., FRIFITERS A., POT J., PELEMAN J., KUIPER M. & XABEAU M. 1995: AFLP: A new technique for DNA fingerprinting. Nucl. Acids Res. 23: 4407-4414 Go to original source...
  66. WAAGE J.K. 1975: Reproductive isolation and the potential for character displacement in the damselflies, Calopteryx maculate and C. aequabilis (Odonata: Calopterygidae). Syst. Zool. 24: 24-36 Go to original source...
  67. WAAGE J.K. 1979: Reproductive character displacement in Calopteryx (Odonata: Calopterygidae). Evolution 33: 104-116 Go to original source...
  68. WARD L. & MILL P.J. 2005: Habitat factors influencing the presence of adult Calopteryx splendens (Odonata: Zygoptera). Eur. J. Entomol. 102: 47-51 Go to original source...
  69. WEEKERS P.H.H., DE JONCKHEERE F.J. & DUMONT H.J. 2001: Phylogenetic relationships inferred from ribosomal ITS sequences and biogeographic patterns in representative of the genus Calopteryx (Insecta: Odonata) of the West Mediterranean and adjacent west European zone. Mol. Phylogen. Evol. 20: 89-99 Go to original source...
  70. WILCOCK H.R., NICHOLS R.A. & HILDREW A.G. 2003: Genetic population structure and neighborhood population size estimates of the caddisfly Plectrocnemia conspersa. Freshwat. Biol. 48: 1813-1824 Go to original source...
  71. WRIGHT S. 1951: The genetic structure of populations. Ann. Eugen. 15: 323-354 Go to original source...
  72. ZHIVOTOVSKY L.A. 1999: Estimating population structure in diploids with multilocus dominant DNA markers. Mol. Ecol. 8: 907-913 Go to original source...