Eur. J. Entomol. 106 (4): 663-665, 2009 | 10.14411/eje.2009.082

Isolation and characterization of polymorphic microsatellite loci in the bamboo locust Rammeacris kiangsu (Orthoptera: Acrididae)

Wen-Juan XUAN, Yan-Yan ZHANG, Na-Na LIU, Jin-Liang ZHAO, Guo-Fang JIANG*
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University,

Twelve polymorphic microsatellite loci were developed and characterized from the bamboo locust, Rammeacris kiangsu, based on enriched genomic libraries. Analysis of 30 individuals showed that the number of alleles ranged from seven to 25 with the observed heterozygosity ranging from 0.333 to 0.767 and expected heterozygosity from 0.784 to 0.963. Test of cross-species amplification showed that some of these microsatellite markers could be used for studying other species such as Ceracris nigricornis, C. fasciata, and Chorthippus brunneus.

Keywords: Orthoptera, Acrididae, Rammeacris kiangsu, microsatellite loci

Received: June 26, 2009; Accepted: August 15, 2009; Published: November 20, 2009

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