Eur. J. Entomol. 106 (3): 451-454, 2009 | DOI: 10.14411/eje.2009.056

Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)

Danijela KOJIC1, Ivan SPASOJEVIC2, Milo MOJOVIC3, Duko BLAGOJEVIC4, M. Roger WORLAND5, Gordana GRUBOR-LAJSIC*,1, Mihajlo B. SPASIC4
1 Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 2, 21000 Novi Sad, Serbia; e-mail: gordanagl@ib.ns.ac.yu
2 Center for Multidisciplinary Studies, University of Belgrade, Serbia
3 Faculty of Physical Chemistry, University of Belgrade, Serbia
4 Department of Physiology, Institute for Biological Research "Sinisa Stankovic", Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
5 British Antarctic Survey, Natural Environment Research Council, High Cross, Madingley Road, Cambridge CB3 0ET, UK

The aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5C, -3C and -16C for two weeks. The amount of hydrogen peroxide (H2O2), activity of antioxidant enzymes, copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutases (MnSOD) and catalase (CAT) in whole body homogenates, as well as the electron paramagnetic resonance (EPR) spectroscopy of this insect's whole body were analysed. A higher level of melanin radical and lower CuZnSOD and CAT activities were found in larvae kept at -3C than at 5C and -16C. At the same temperature (-3C) an elevated H2O2 concentration was recorded. A possible regulatory role of H2O2 at -3C, which is the temperature that triggers freezing tolerance, is suggested.

Keywords: Ostrinia nubilalis, antioxidant enzymes, melanin, hydrogen peroxide, cold-hardiness

Received: February 5, 2009; Accepted: April 14, 2009; Published: July 24, 2009

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