Eur. J. Entomol. 106 (3): 325-334, 2009 | 10.14411/eje.2009.040

Genetic diversity of the xerothermic weevils Polydrusus inustus and Centricnemus leucogrammus (Coleoptera: Curculionidae) in central Europe

Lukasz KAJTOCH1, Dorota LACHOWSKA-CIERLIK2, Mieczyslaw MAZUR3
1 Institute of Systematics and Evolution of Animals, Polish Academy of Science, Sławkowska 17 St., Krakow, Poland, e-mail: kajtoch@isez.pan.krakow.pl
2 Institute of Zoology, Department of Entomology, Jagiellonian University, Ingardena 6 St., Krakow, Poland
3 Institute of Biology, Pedagogical Academy, Podbrzezie 3, Krakow, Poland

Phylogeography, genetic diversity, and demography of central European populations of two flightless xerothermic weevils, Polydrusus inustus and Centricnemus leucogrammus, were studied based on the polymorphism of three mtDNA genes (COII, CytB, and ND1). Results indicate that these xerothermic beetles may have different origins. P. inustus is a recent migrant as the parthenogenetic form has a low level of genetic diversity and lacks a geographic population structure. This is probably a result of a recent (before the end of last glaciation) expansion and/or present dispersal mediated by humans. On the other hand, C. leucogrammus appears to be a relic species as the populations of this species are much more genetically diverse (six distinct clades) and some of the populations are allopatric and others sympatric. They probably diverged and expanded during the last few glaciations. Genetic discontinuities were detected among localities that are now separated by gaps in the distribution. Boundaries (mountains or farmland) separate the populations into three groups: (1) Moravia and Slovakia, (2) the lower Vistula Valley in northern Poland and (3) south-eastern Poland together with western Ukraine. Evidence for recent gene flow was found only among populations from south-eastern Poland and western Ukraine, and between these two groups. One population from northern Poland was surprisingly related to populations in southern Poland, which may be due to extinction of intermediate populations.

Keywords: Curculionidae, weevils, mtDNA, phylogeography, xerothermic habitats

Received: December 8, 2008; Accepted: April 21, 2009; Published: July 24, 2009

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References

  1. AVISE J.C. 2000: Phylogeography: The History and Formation of Species. Harvard University Press, Cambridge, Massachusetts, viii + 447 pp
  2. BARRACLOUGH T.G. & VOGLER A.P. 2002: Recent diversification rates in North American tiger beetles estimated from a dated mtDNA phylogenetic tree. Mol. Biol. Evol. 19: 1706-1716 Go to original source...
  3. BEAUMONT M.A. & PANCHAL M. 2008: On the validity of nested clade phylogeography analysis. Mol. Ecol. 17: 2563-2565 Go to original source...
  4. BERECZKI J., PECSENYE K., PEREGOVIS L. & VARGA Z. 2005: Pattern of genetic differentiation in the Maculinea alcon species group (Lepidoptera, Lycaenidae) in Central Europe. Syst. Zool. Evol. Res. 43: 157-165 Go to original source...
  5. BHAGWAT S.A. & WILLIS K.J. 2008: Species persistence in northerly glacial refugia of Europe: a matter of chance or biogeographical traits? J. Biogeogr. 35: 464-482 Go to original source...
  6. CACCONE A. & SBORDONI V. 2001: Molecular biogeography, evolutionary rates, and morphological adaptation to cave life: a case study using Bathysciinae beetles and sequence data from the mitochondrial COI gene. Evolution 55: 122-130 Go to original source...
  7. CLEMENT M., POSADA D. & CRANDALL K.A. 2000: TCS: a computer program to estimate gene genealogies. Mol. Ecol. 9: 1657-1659 Go to original source...
  8. COCKERHAM C.C. & WEIR B.S. 1993: Estimation of gene flow from F-statistics. Evolution 47: 855-863 Go to original source...
  9. CONORD C., LEMPERIERE G., TABERLET P. & DESPRES L. 2006: Genetic structure of the forest pest Hylobius abietis on conifer plantations at different spatial scales in Europe. Heredity 97: 46-55 Go to original source...
  10. COOPE G.R. 1994: The response of insect faunas to glacialinterglacial climatic fluctuations. Philosophical Trans. R. Soc. Lond. (B) 344: 19-26 Go to original source...
  11. DUPANLOUP I., SCHNEIDER S. & EXCOFFIER L. 2002: A simulated annealing approach to define the genetic structure of populations. Mol. Ecol. 11: 2571-2581 Go to original source...
  12. EMERSON B.C., PARADIS E. & THEBAUD C. 2001: Revealing the demographic histories of species using DNA sequences. Trends Ecol. Evol. 16: 707-716 Go to original source...
  13. FACCOLI M., BLAzENEC M. & SCHLYTER F. 2005: Feeding response to host and non-host compounds by males and females of the spruce bark beetle Ips typographus in a tunnelling microassay. J. Chem. Ecol. 31: 745-759 Go to original source...
  14. FARRELL B.D. 2001: Evolutionary assembly of the milkweed fauna: cytochrome oxidase I and the age of tetraopes beetles. Mol. Phylogen. Evol . 18: 467-478 Go to original source...
  15. FARRIS J.S., KALLERSJO M., KLUGE A.G. & BULT C. 1995: Constructing a significance test for incongruence. Syst. Biol. 44: 570-572 Go to original source...
  16. FU X.-Y. 1997: Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics 147: 915-925
  17. GARNIER S., ALIBERT P., AUDIOT P., PRIEUR B. & RASPLUS J.-Y. 2004: Isolation by distance and sharp discontinuities in gene frequencies: implications for the phylogeography of an alpine insect species, Carabus solieri. Mol. Ecol. 13: 1883-1897 Go to original source...
  18. GARRICK R.C., DYER R.J., BEHEREGARAY L.B. & SUNNUCKS P. 2008: Babies and bathwater: a comment on the premature obituary for nested clade phylogeographic analysis. Mol. Ecol. 17: 1401-1403 Go to original source...
  19. GRAPPUTO A., BOMAN S., LINDSTROM L., LYYTINEN A. & MAPPES J. 2005: The voyage of an invasive species across continents: genetic diversity of North American and European Colorado potato beetle populations. Mol. Ecol. 14: 4207-4219 Go to original source...
  20. GRATTON P., KONOPINSKI M.K. & SBORDONI S.V. 2008: Pleistocene evolutionary history of the clouded Apollo (Parnassius mnemosyne): genetic signatures of climate cycles and a "time-dependent" mitochondrial substitution rate. Mol. Ecol. 17: 4248-4262 Go to original source...
  21. HALL T.A. 1999: BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp. Ser. (Lond.) 41: 95-98
  22. HASEGAWA M., KISHINO H. & YANO T. 1985: Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. J. Mol. Evol. 22: 160-174 Go to original source...
  23. HEWITT G.M. 1999: Post-glacial re-colonization of European biota. Biol. J. Linn. Soc. 68: 87-112 Go to original source...
  24. HUELSENBECK J.P. & RONQUIST F. 2001: MRBAYES: Bayesian inference of phylogeny. Bioinformatics 17: 754-755 Go to original source...
  25. JUAN C., OROMI P. & HEWITT G.M. 1996: Phylogeny of the genus Hegeter (Tenebrionidae: Coleoptera) and its colonization of the Canary Islands deduced from cytochrome oxidase I mitochondrial DNA sequences. Heredity 77: 392-403 Go to original source...
  26. KAJTOCH L. & LACHOWSKA-CIERLIK D. (in press). Genetic constitution of parthenogenetic form of Polydrusus inustus (Coleoptera: Curculionidae) - hints of hybrid origin and recombinations. Folia Biol. (Krakow)
  27. KOROTYAEV B.A. 1996: Use of data on distribution of the bisexual and parthenogenetic forms of weevils for faunogenetic reconstructions (Coleoptera, Curculionidae). In: Verhandlungen des 14. Internationalen Symposiums fur Entomofaunistik in Mitteleuropa, SIEEC, in Munchen (04.-09.09.1994). Munchen, pp. 264-271
  28. KOWALSKI K. (ed.) 1989: Historia i Ewolucja Ladowej Fauny Polski. [History and evolution of the terrestrial fauna of Poland.] PAN, Ossolineum, Krakow, 278 pp. [in Polish, English abstr.]
  29. KNOWLES L.L. & MADDISON W.P. 2002: Statistical phylogeography. Mol. Ecol. 11: 2623-635 Go to original source...
  30. LACHOWSKA D., ROzEK M., KAJTOCH L. & HOLECOVA M. 2006: Cytogenetic differences between Peritelus familiaris and Centricnemus leucogrammus (Coleoptera: Curculionidae: Entiminae: Peritelini). Eur. J. Entomol. 103: 687-690. Go to original source...
  31. LACHOWSKA D., ROzEK M. & HOLECOVA M. 2008: New data on the cytology of parthenogenetic weevils (Coleoptera, Curculionidae). Genetica 134: 235-242 Go to original source...
  32. LAFFIN R.D., DOSDALL L.M. & SPERLING F.A.H. 2005: Population structure of the cabbage seedpod weevil, Ceutorhynchus obstrictus (Marsham) (Coleoptera: Curculionidae): Origins of North American introductions. Environ. Entomol. 34: 504-510 Go to original source...
  33. LAKATOS F., GRODZKI W., ZHANG Q.-H. & STAUFFE C. 2007: Genetic comparison of Ips duplicatus (Sahlberg, 1836) (Coleoptera: Curculionidae, Scolytinae) populations from Europe and Asia. J. Forest Res. 12: 345-349 Go to original source...
  34. LIANA A. 1987: Orthoptera of xerothermic habitats in Poland and their origin. In Baccetti B. (ed.): Evolutionary Biology of Orthopteroid Insects. Ellis Horwood, Chichester, pp. 342-346
  35. MANTEL N. 1967: The detection of disease clustering and a generalized regression approach. Cancer Res. 27: 209-220
  36. MARDULYN P. 2001: Phylogeography of the Vosges mountains populations of Gonioctena pallida (Coleoptera: Chrysomelidae): a nested clade analysis of mitochondrial DNA haplotypes. Mol. Ecol. 10: 1751-1763 Go to original source...
  37. MAROJA L.S., BOGDANOWICZ S.M., WALLIN K.F., RAFFA K.F. & HARRISON R.G. 2007: Phylogeography of spruce beetles (Dendroctonus rufipennis Kirby) (Curculionidae: Scolytinae) in North America. Mol. Ecol. 16: 2560-2573 Go to original source...
  38. MAZUR M. 1994: Rozmieszczenie i migracje Polydrusus inustus Germar, 1824 (Coleoptera: Curculionidae). Pol. Pis. Entomol. 63: 113-132
  39. MAZUR M. 2001: Ryjkowce kserotermiczne Polski (Curculionoidea: Nemonychidae, Attelabidae, Apionidae, Curculionidae). Studium zoogeograficzne. Monografie Fauny Polski 22: 1-378
  40. NEHRING A. 1890: Ueber Tundren und Steppen der Jetzt -und Vorzeit. Duemmler, Berlin, viii + 257 pp
  41. NEI M. 1987: Molecular Evolutionary Genetics. Columbia University Press, New York, NY, x + 512 pp
  42. PANCHAL M. 2007: The automation of nested clade phylogeographic analysis. Bioinformatics 23: 509-510 Go to original source...
  43. PANCHAL M. & BEAUMONT M.A. 2007: The automation and evaluation of nested clade phylogeographic analysis. Evolution 61: 1466-1480 Go to original source...
  44. PETRYSZAK B. 1977: A karyological study of five bisexual species of weevils (Curculionidae, Coleoptera). Zool. Polon. 26: 475-482
  45. PETIT R.J. & GRIVET D. 2002: Optimal randomization strategies when testing the existence of a phylogeographic structure. Genetics 161: 469-471
  46. PFENNINGER M. & POSADA D. 2002: Phylogeographic history of the land snail Candidula unifasciata (Poiret, 1801) (Helicellinae, Stylommatophora): fragmentation, corridor migration and secondary contact. Evolution 56: 1776-1788 Go to original source...
  47. PFENNINGER M., POSADA D. & FREDERIC M. 2003: Evidence for survival of Pleistocene climatic changes in Northern refugia by the land snail Trochoidea geyeri (Soos, 1926) (Helicellinae, Stylommatophora). BMC Evol. Biol. 3: 8 Go to original source...
  48. POSADA D. & CRANDALL K.A. 1998: Modeltest: testing the model of DNA substitution. Bioinformatics 14: 817-818 Go to original source...
  49. POSADA D., CRANDALL K.A. & TEMPLETON A.R. 2000: Geodis: a program for the cladistic nested analysis of the geographical distribution of genetic haplotypes. Mol. Ecol. 9: 487-488 Go to original source...
  50. RIBERA I., HERNANDO C. & AQUILERA P. 2001: Agapus alexandae sp.n. from Marocco, with a molecular phylogeny of the Western Mediterranean species of the A. gattutus group (Coleoptera: Dytiscidae). Insect Syst. Evol. 32: 253-262 Go to original source...
  51. RITZEROW S., KONRAD H. & STAUFFER C. 2004: Phylogeography of the Eurasian pine shoot beetle Tomicus piniperda L. (Coleoptera, Scolytidae). Eur. J. Entomol. 101: 13-19 Go to original source...
  52. ROGERS A.R. & HARPENDING H. 1992: Population growth makes waves in the distribution of pairwise genetic differences. Mol. Biol. Evol. 9: 552-569
  53. RONQUIST F. & HUELSENBECK J.P. 2003: MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572-1574 Go to original source...
  54. ROZAS J., SANCHEZ-DELBARRIO J.C., MESSEGUER X. & ROZAS R. 2003: DNASP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics 19: 2496-2497 Go to original source...
  55. SCHNEIDER S., ROESSLI D. & EXCOFFIER L. 2000: ARLEQUIN: A Software for Population Genetics Data Analysis. University of Geneva, Geneva, Switzerland
  56. SIMON C., FRATI F., BECHENBACH A., CRESPI B., LIU H. & FLOCK P. 1994: Evolution, weighting, and phylogenetic utility of mitochondrial gene sequence and compilation of conserved polymerase chain reaction primers. Ann. Entomol. Soc. Am. 87: 651-701 Go to original source...
  57. SLATKIN M. 1985: Rare alleles as indicators of gene flow. Evolution 39: 53-65 Go to original source...
  58. SLATKIN M. 1993: Isolation by distance in equilibrium and nonequilibrium populations. Evolution 47: 264-279 Go to original source...
  59. STAUFFER C., LAKATOS F. & HEWITT G.M. 1999: Phylogeography and postglacial colonization routes of Ips typographus L. (Coleoptera, Scolytidae). Mol. Ecol. 8: 763-773 Go to original source...
  60. SWOFFORD D.L. 2002: PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Sinauer Associates, Sunderland, MA
  61. TABERLET P., FUMAGALLI L., WUST-SAUCY A.G. & COSSON J.F. 1998: Comparative phylogeography and postglacial colonisation routes in Europe. Mol. Ecol. 7: 453-464 Go to original source...
  62. TARASOV P.E., VOLKOVA V.S., WEBB III T., GUIOT J., ANDREEV A.A., BEZUSKO L.G., BEZUSKO T.V., BYKOVA G.V., DOROFEYUK N.I., KVAVADZE E.V., OSIPOVA I.M., PANOVA N.K. & SEVASTYANOV D.V. 2000: Last glacial maximum biomes reconstructed from pollen and plant macrofossil data from northern Eurasia. J. Biogeogr. 27: 609-620 Go to original source...
  63. TEMPLETON A.R. 1998: Nested clade analysis of phylogeographic data: Testing hypotheses about gene flow and population history. Mol. Ecol. 7: 381-397 Go to original source...
  64. TEMPLETON A.R. 2001: Using phylogeographic analyses of genetrees to test species status and processes. Mol. Ecol. 10: 779-791 Go to original source...
  65. TEMPLETON A.R. 2004: Statistical phylogeography: methods of evaluating and minimizing inference errors. Mol. Ecol. 13: 789-809 Go to original source...
  66. TEMPLETON A.R., CRANDALL K.A. & SING C.F. 1992: A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data. III. Cladogram estimation. Genetics 132: 619-633
  67. THOMPSON J.D., GIBSON T.J., PLEWNIAK F., JEANMOUGIN F. & HIGGINS D.G. 1997: The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl. Acids Res. 24: 4876-4882 Go to original source...
  68. VARGA Z. 2003: Biodiversity and Phylogeography. Acta Biol Debr. 24: 5-38
  69. WILLIS K.J. & VAN ANDEL T.A. 2004: Trees or no trees? The environments of central and eastern Europe during the Last Glaciation. Quat. Sci. Rev. 23: 2369-2387 Go to original source...
  70. WRIGHT S. 1951: The genetic structure of populations. Ann. Eugen. 15: 323-354 Go to original source...