Eur. J. Entomol. 106 (2): 185-191, 2009 | 10.14411/eje.2009.022

Purification and characterization of a novel thermoacidophilic and thermostable α-mannosidase from the digestive fluid of oil palm weevil Rhynchophorus palmarum (Coleoptera: Curculionidae) larvae

Micaël BÉDIKOU1, Pascal AHI2, Martial KONÉ3, Betty FAULET2, Jean GONNETY2, Patrice KOUAMÉ2, Sébastien NIAMKÉ*,1,2
1 Laboratoire de Biotechnologies, Filière Biochimie-Microbiologie de l'Unité de Formation et de Recherche en Biosciences de l'Université de Cocody-Abidjan, 22 BP 582 Abidjan 22, Côte d'Ivoire
2 Laboratoire de Biochimie et Technologie des Aliments de l'Unité de Formation et de Recherche en Sciences et Technologie des Aliments de l'Université d'Abobo-Adjamé, 02 BP 801 Abidjan 02, Côte d'Ivoire
3 Unité de Biotechnologie, Biocatalyse et Biorégulation, CNRS-UMR 6204, Laboratoire de Biochimie, Faculté des Sciences et Techniques, 2 Rue de la Houssinière, BP 92208, F-44322 Nantes Cedex 3, France

An extracellular α-mannosidase with unusual properties was purified from the digestive fluid of oil palm weevil (Rhynchophorus palmarum Linnaeus) larvae using ammonium sulphate saturation, size exclusion and anion-exchange chromatography. The enzyme named RpltM is thermoacidophilic, thermostable and behaves like lysosomal α-mannosidase (EC 3.2.1.24). The molecular weight, Km value, optimum reaction temperature and pH are 108-112 kDa, 0.36 mM, 65°C and 4.5, respectively. Zn2+ enhanced whereas Cu2+, Sodium dodecyl sulphate, swainsonine and 1,4-dideoxy-1,4-iminomannitol strongly inhibited its hydrolytic activity. The enzyme was stable for 25 min at 65°C and retained 70% of its initial activity after 60 min. At 70°C, around 60% of this activity was conserved after 25 min. RpltM retained more than 90% of its activity over a pH range of 4.2 to 5.0 and remained fully active in the presence of detergents such as nonidet P-40, triton X-100, polyoxyethylen-10-oleyl ether (up to 1%, w/v), dithiothreitol and β-mercaptoethanol. The stability under these conditions is also better than that reported for other insect α-mannosidases. Thus, RpltM could be used as an important bioindustrial tool for removing mannose residues from oligosaccharides.

Keywords: a-mannosidase, thermoacidophilic, thermostable, oil palm weevil, Rhynchophorus palmarum, Curculionidae

Received: October 17, 2008; Accepted: January 5, 2009; Published: May 20, 2009

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