Eur. J. Entomol. 106 (1): 29-35, 2009 | DOI: 10.14411/eje.2009.004

Proteomic analysis of pre-diapause, diapause and post-diapause larvae of the wheat blossom midge, Sitodiplosis mosellana (Diptera: Cecidomyiidae)

Wei-Ning CHENG1, Xiu-Lian LI1, Feng YU2, Yi-Ping LI1, Jian-Jun LI1, Jun-Xiang WU*,1
1 The College of Plant Protection, Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Northwest A & F University, Yangling, 712100 Shaannxi, China; e-mail: junxw@yahoo.com.cn
2 The College of Animal Science and Technology, Northwest A & F University, Yangling, 712100 Shaannxi, China

To determine the relationship between protein expression and insect diapause, a proteomic approach was used to investigate the proteins extracted from larvae of the wheat blossom midge Sitodiplosis mosellana Gehin at different developmental stages, including pre-diapause, over-summering diapause, over-wintering diapause and post-diapause. Using 2-DE gels stained with coomassie brilliant blue, about 300 protein spots were detected in the extracts of pre-diapause larvae and 275 for those in each of the other stages. There were 91, 92 and 95 protein spots that showed more than a 2-fold change in abundance in the over-summering diapause, over-wintering diapause and post-diapause stages compared with pre-diapause. Eight protein spots, which showed the greatest difference in the larvae at different stages of diapause, were analyzed using Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Seven of them were successfully identified from their peptide mass fingerprints using the NCBInr database. They were proopiomelanocortin, NADH dehydrogenase subunit 1 and F10F2.5, which were up-regulated or unique to pre-diapause larvae, IKK interacting protein isoform 2 up-regulated in diapause and post-diapause larvae, GA10647-PA unique in over-wintering diapause larvae, purple CG16784-PB isoform B and B0228.6 up-regulated in over-summering and over-wintering diapause larvae. The potential functions of these proteins during wheat blossom midge diapause are discussed.

Keywords: Sitodiplosis mosellana, diapause, proteome, 2-dimensional electrophoresis, mass spectrometry

Received: August 19, 2008; Accepted: November 13, 2008; Published: March 10, 2009

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