Eur. J. Entomol. 105 (5): 945-948, 2008 | 10.14411/eje.2008.121

Microsatellites for the highly invasive Cameraria ohridella (Lepidoptera: Gracillariidae) and their amplification in related species

Neus MARI MENA, Romain VALADE, Emmanuelle MAGNOUX, Sylvie AUGUSTIN, Carlos LOPEZ-VAAMONDE*
INRA, URZF 633, Centre d'Orléans, Unité de Zoologie Forestière, 2163 Avenue de la Pomme de Pin; CS 40001 Ardon, 45075

A set of six polymorphic microsatellite markers was developed for the highly invasive horse chestnut leaf-mining moth, Cameraria ohridella. For 96 positive clones, six polymorphic microsatellites with conserved flanking regions were identified. The average number of alleles per locus was eight (range from three to 12). Two of these loci showed significant heterozygosity deficits due to null alleles. The remaining four loci did not depart from Hardy-Weinberg equilibrium. In addition, all six loci were amplified for 20 other gracillariid species belonging to eight different genera, including another invasive species Phyllonorycter platani. These are the first microsatellite markers described for a species within the lepidopteran family Gracillariidae.

Keywords: Gracillariidae, Aesculus hippocastanum, Cameraria ohridella, horse chestnut leafminer, invasion, microsatellites, Phyllonorycter platani

Received: August 13, 2008; Accepted: September 5, 2008; Published: December 30, 2008

Download citation

References

  1. ANDERSON S.J., DAWSON D.A. & FREELAND J.R. 2006: Isolation and characterization of highly polymorphic microsatellite loci for the garden tiger moth Arctia caja (Lepidoptera: Arctiidae). Mol. Ecol. Notes 6: 104-106 Go to original source...
  2. ARMOUR J.A., NEUMANN R., GOBERT S. & JEFFREYS A.J. 1994: Isolation of human simple repeat loci by hybridization selection. Hum. Mol. Genet. 3: 599-565 Go to original source...
  3. CASSEL-LUNDHAGEN A. 2002: Characterization of microsatellite loci in Coenonympha hero (Lepidoptera: Nymphalidae). Mol. Ecol. Notes 2: 566-568 Go to original source...
  4. GAUTSCHI B., TENZER I., MULLER J.P. & SCHMID B. 2000a: Isolation and characterization of microsatellite loci in the bearded vulture (Gypaetus barbatus) and crossamplification in three Old World vulture species. Mol. Ecol. 9: 2193-2195 Go to original source...
  5. GAUTSCHI B., WIDMER A. & KOELLA J. 2000b: Isolation and characterization of microsatellite loci in the Dice Snake (Natrix tessellata). Mol. Ecol. 9: 2191-2193 Go to original source...
  6. HABEL J.C., FINGER A., MEYER M., SCHMITT T. & ASSMANN T. 2008: Polymorphic microsatellite loci in the endangered butterfly Lycaena helle (Lepidoptera: Lycaenidae). Eur. J. Entomol. 105: 361-362 Go to original source...
  7. KAWAKITA A. & KATO M. 2006: Assessment of the diversity and species specificity of the mutualistic association between Epicephala moths and Glochidion trees. Mol. Ecol. 15: 3567-3581 Go to original source...
  8. LOPEZ-VAAMONDE C., GODFRAY H.C.J. & COOK J.M. 2003: Evolutionary dynamics of host-plant use in a genus of leaf-mining moths. Evolution 57: 1804-1821 Go to original source...
  9. MARSHALL T.C., SLATE J., KRUUK L. & PEMBERTON J.M. 1998: Statistical confidence for likelihood-based paternity inference in natural populations. Mol. Ecol. 7: 639-655 Go to original source...
  10. MEGLECZ E., PETENIAN F., DANCHIN E., COEUR D'ACIER A., RASPLUS J.-Y. & FAURE E. 2004: High similarity between flanking regions of different microsatellites detected within each of two species of Lepidoptera: Parnassius apollo and Euphydryas aurinia. Mol. Ecol. 13: 1693-1700 Go to original source...
  11. MEGLECZ E., ANDERSON A., BOURGET D., BUTCHER R., CALDAS A., CASSEL-LUNDHAGEN A., COEUR D'ACIER A., DAWSON A.D., FAURE N., FAUVELOT C., FRANCK P., HARPER G., KEYGHOBADI N., KLUETSCH C., MUTHULAKSHMI M., NAGARAJU J., PATT A., PETENIAN F., SALIVAIN J.F. & WILCOCK H. 2007: Microsatellite flanking region similarities among different loci within insect species. Insect Mol. Biol. 16: 175-185 Go to original source...
  12. RAYMOND M. & ROUSSET F. 1995: Genepop (version 1.2): Population genetics software for exact tests and ecumenicism. J. Hered. 86: 248-249. Go to original source...
  13. SEFROVA H. 2001: Phyllonorycter platani (Staudinger) - a review of its dispersal history in Europe (Lepidoptera, Gracillariidae). Acta Univ. Agric. Silvic. Mendel. Brun. 49: 71-75
  14. SELKOE K.A. & TOONEN R.J. 2006: Microsatellites for ecologists: a practical guide to using and evaluating microsatellite markers. Ecol. Lett. 9: 615-629 Go to original source...
  15. VAN OOSTERHOUT C., HUTCHINSON W.F., WILLS D.P.M. & SHIPLEY P. 2004: Micro-checker: Software for identifying and correcting genotyping errors in microsatellite data. Mol. Ecol. Notes 4: 535-538 Go to original source...