Eur. J. Entomol. 105 (4): 707-712, 2008 | DOI: 10.14411/eje.2008.096

Morphology and ultrastructure of the male reproductive system of the woolly beech aphid Phyllaphis fagi (Hemiptera: Aphididae: Phyllaphidinae)

1 Department of Zoology,
2 Department of Histology and Embryology of Animals, Faculty of Biology and Environmental Protection, University of Silesia, Bankowa 9, 40-007 Katowice, Poland; e-mails:;

In the present paper we describe for the first time the ultrastructure of the male reproductive system of aphids using Phyllaphis fagi as a representative. Paired testes of this species consist of three follicles each, arranged in a rosette, the walls of the proximal part of vasa deferentia cling together, accessory glands are club-shaped and elongated whereas the ejaculatory duct is reduced. Our study also shows that vasa deferentia, accessory glands and ejaculatory duct are histologically very simple. All of them are composed of cubical epithelium of secretory type. The epithelial cells are rich in rough endoplasmic reticulum, Golgi complexes and produce small heterogeneous vacuoles. The apical membrane of these cells forms microvilli. The reproductive system epithelia stand on thin basal lamina. Outside the basal lamina thin muscle fibres are observed. Histochemical staining shows that secretion filling the lumen of vasa deferentia and accessory glands contains proteins and polysaccharides.

Keywords: Aphididae, Phyllaphidinae, Phyllaphis fagi, male reproductive system, ultrastructure

Received: November 28, 2007; Accepted: January 25, 2008; Revised: January 25, 2008; Published: October 24, 2008Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
WIECZOREK, K., & SWIĄTEK, P. (2008). Morphology and ultrastructure of the male reproductive system of the woolly beech aphid Phyllaphis fagi (Hemiptera: Aphididae: Phyllaphidinae). Eur. J. Entomol.105(4), 707-712. doi: 10.14411/eje.2008.096.
Download citation


  1. BLACKMAN R.L. 1987: Reproduction cytogenetics and development. In Minks A.K. & Harrewijn P. (eds): Aphids, their Biology, Natural Enemies and Control. Elsevier, Amsterdam, pp. 163-197
  2. BLACKMAN R.L. & EASTOP V.F. 1994: Aphids on the World's Trees. An Identification and Information Guide. Cambridge University Press, Cambridge, 986 pp
  3. BORNER C. 1952: Europae Centralis Aphides die Blattlaeuse Mitteleuropas Namen, Synonyme, Wirtspflanzen, Generationszyklen. Mitt Thuering Bot. Ges. 3. Vols 1, 2. Weimar, 488 pp
  4. CHAPMANN R.F. 2004: The Insects. Structure and Function. Cambridge University Press, Cambridge, 770 pp
  5. EASTOP V.F. 1977: Worldwide of aphids as virus vector. In Harris K.F. & Maramorosch K. (eds): Aphids as Virus Vectors. Academic Press, New York, San Francisco, London, pp. 1-63 Go to original source...
  6. GILLOT C. 1988: Arthropoda - Insecta. In Adiyodi K.G.& Adiyodi R.G. (eds): Reproductive Biology of Invertebrates. III. Accessory Sex Glands. Wiley & Sons, Chichester, pp. 319-471
  7. GLOWACKA E., KLIMASZEWSKI S.M., SZELEGIEWICZ H. & WOJCIECHOWSKI W. 1974: Uber den Bau des mannlichen Fortpflanzungssystems der Aphiden (Homoptera, Aphidoidea). Ann. Univ. Mariae Curie-Skaodowska (Lublin) (C) 29: 133-138
  8. HAPP G.M. 1984: Structure and development of male accessory glands. In King R.C. & Akai H. (eds): Insect Ultrastructure. Vol. 2. Plenum Press, New York, pp. 365-396
  9. HEIE O.E. 1982: The Aphidoidea (Hemiptera) of Fennoscandia and Denmark. II. The Family Drepanosiphidae. Fauna Entomologica Scandinavica. Vol. 11. Sciandinavian Science Press, Klampenborg, 175 pp
  10. IVERSEN T. & HARDING S. 2007a: Life table parameters affecting the population development of the woolly beech aphid, Phyllaphis fagi L. Entomol. Exp. Appl. 123: 109-117 Go to original source...
  11. IVERSEN T. & HARDING S. 2007b: Biological and other alternative control methods against the woolly beech aphid Phyllaphis fagi L. on beech Fagus sylvatica seedlings in forest nurseries. J. Pest Sci. 80: 159-166 Go to original source...
  12. KLIMASZEWSKI S.M., SZELEGIEWICZ H. & WOJCIECHOWSKI W. 1973: Uber den Bau des mannlichen Fortpflanzungssystems von Drepanosiphum platanoidis (Schr.) (Homoptera, Aphidoidea). Bull. Acad. Pol. Sci. 10: 671-674
  13. NIETO NAFRIA J.M. & MIER DURANTE M.P. 1998: Fauna Iberica 11. Hemiptera: Aphididae I. Museo Nacional, Madrid, 425 pp
  14. PRABUCKI J. 1972: Z badan nad biologia spadziodajnej mszycy Phyllaphis fagi L. (Homoptera). [Studies on honeydew aphid Phyllaphis fagi L. (Homoptera).] Pol. Pis. Entomol. 42: 258-269 [in Polish]
  15. QUEDNAU F.W. & REMAUDIERE G. 1994: Le genre sud-americain Neuquenaphis E.E. Blanchard, description de deux nouvelles especes et definition de nouvelles sous-familles d'Aphididae (Homoptera). Bull. Soc. Entomol. Fr. 99: 365-384
  16. REMAUDIERE G. & REMAUDIERE M. 1997: Catalogue of the World's Aphididae (Homoptera, Aphidoidea). INRA Editions, Versailles, 473 pp
  17. STROYAN H.L.G. 1977: Homoptera: Aphidoidea: Chaitophoridae and Callaphididae. Handbooks for the Identification of British Insects. Vol II. Part 4(a). Royal Entomological Society of London, London, 130 pp
  18. WIECZOREK K. 2006: Anatomical investigations of the male reproductive system of five species of Calaphidinae (Hemiptera, Aphidoidea). Insect System. Evol. 37: 457-465 Go to original source...
  19. WIECZOREK K. 2008: Structure of the male reproductive system of Anoecia (Anoecia) corni Fabricius, 1775 (Hemiptera, Aphidoidea), a representative of the family Anoeciidae. Acta Zool. 89: 163-167 Go to original source...
  20. WIECZOREK K. & SWIATEK P. 2007: Classification of the drepanosiphine aphids (Hemiptera, Aphidoidea: Phyllaphidinae, Calaphidinae, Drepanosiphinae) in the light of the anatomy research. Int. J. Invert. Taxonomy - Genus (Suppl.) 14: 63-65
  21. WIECZOREK K. & WOJCIECHOWSKI W. 2004: The systematic position of Chaitophorinae (Hemiptera, Aphidoidea) in the light of anatomy research. Insect System. Evol. 35: 317-327 Go to original source...
  22. WOJCIECHOWSKI W. 1977: Procesy oligomeryzacji w budowie meskiego ukladu rozrodczego miodownic (Homoptera, Lachnidae). [Oligomerisation processes in the structure of male genitalia in Lachnidae (Homoptera)]. Prace Naukowe USl. (Katowice) 3: 140-164 [in Polish]

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY), which permits use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.